Cancer is a pathogenic condition due to abnormal growth of cells,which is one of the leading reasons for the death of majority of the population throughout the world.Pharmacotherapy associated with the majority of the...Cancer is a pathogenic condition due to abnormal growth of cells,which is one of the leading reasons for the death of majority of the population throughout the world.Pharmacotherapy associated with the majority of the adverse effects such as cardiotoxicity,hepatotoxicity,nephrotoxicity that suggests identifying new drug which would be safer than well-established chemotherapy.There is a need to get a promising line for research over various diseases including cancer.Gloriosa superba Linn has a long history of use in folk medicine including wounds,hemorrhoids,kidney problems,and tumors.The present study explores the ethanolic extract of Gloriosa superba Linn as the anti-cancer agent using in vitro MTT assay and in vivo Ehrlich’s ascites carcinoma(EAC)bearing Balb/c mice models.Further,the study reports the IC50 value of the DPPH was 1.21μg/mL,and the IC50 of MTT assay against Hela and MCF7 was found to be 148.50μg/mL and 147.23μg/mL respectively.For,in vivo study,animals were divided into five groups(n=6)experimented for 14 days.The tumor was induced by intraperitoneal transplantation of Ehrlich’s ascites carcinoma cells in Balb/c mice.The normal and control group received normal saline.After 24 hours of Ehrlich’s Ascites Carcinoma transplantation,treatment was started by injecting Cyclophosphamide(50 mg/kg)intraperitoneal whereas Gloriosa superba Linn extract(5 mg/kg&10 mg/kg)was administrated orally.On the 15th day,mice were sacrificed to estimate hematology,biochemical estimation,and histopathology.The extract showed a significant decrease(P<0.001)in body weight in the treatment group compare to the control group further there was amelioration in hematology,biochemical estimation,and histopathology.From the result,it was concluded that the extract has a potent antitumor activity.展开更多
文摘Cancer is a pathogenic condition due to abnormal growth of cells,which is one of the leading reasons for the death of majority of the population throughout the world.Pharmacotherapy associated with the majority of the adverse effects such as cardiotoxicity,hepatotoxicity,nephrotoxicity that suggests identifying new drug which would be safer than well-established chemotherapy.There is a need to get a promising line for research over various diseases including cancer.Gloriosa superba Linn has a long history of use in folk medicine including wounds,hemorrhoids,kidney problems,and tumors.The present study explores the ethanolic extract of Gloriosa superba Linn as the anti-cancer agent using in vitro MTT assay and in vivo Ehrlich’s ascites carcinoma(EAC)bearing Balb/c mice models.Further,the study reports the IC50 value of the DPPH was 1.21μg/mL,and the IC50 of MTT assay against Hela and MCF7 was found to be 148.50μg/mL and 147.23μg/mL respectively.For,in vivo study,animals were divided into five groups(n=6)experimented for 14 days.The tumor was induced by intraperitoneal transplantation of Ehrlich’s ascites carcinoma cells in Balb/c mice.The normal and control group received normal saline.After 24 hours of Ehrlich’s Ascites Carcinoma transplantation,treatment was started by injecting Cyclophosphamide(50 mg/kg)intraperitoneal whereas Gloriosa superba Linn extract(5 mg/kg&10 mg/kg)was administrated orally.On the 15th day,mice were sacrificed to estimate hematology,biochemical estimation,and histopathology.The extract showed a significant decrease(P<0.001)in body weight in the treatment group compare to the control group further there was amelioration in hematology,biochemical estimation,and histopathology.From the result,it was concluded that the extract has a potent antitumor activity.
