以新疆小白杏为试材,采用3 g/100 mL CaCl_(2)或蒸馏水(CK组)雾化熏蒸处理,测定小白杏在(10±1.0)℃、相对湿度(80±5)%条件下果皮、果肉中叶绿素、类胡萝卜素、腺苷三磷酸(adenosine triphosphate,ATP)、二磷酸腺苷(adenosine ...以新疆小白杏为试材,采用3 g/100 mL CaCl_(2)或蒸馏水(CK组)雾化熏蒸处理,测定小白杏在(10±1.0)℃、相对湿度(80±5)%条件下果皮、果肉中叶绿素、类胡萝卜素、腺苷三磷酸(adenosine triphosphate,ATP)、二磷酸腺苷(adenosine diphosphate,ADP)、单磷酸腺苷(adenosine monophosphate,AMP)含量和能荷的变化,分析能量代谢相关酶活力及相关基因的表达量变化,探究外源CaCl_(2)处理对小白杏采后能量代谢的调控机制,明确能量代谢水平与转色之间的联系。结果表明,与CK组相比,CaCl_(2)处理小白杏可延缓果皮、果肉中叶绿素降解,抑制类胡萝卜的合成,维持果皮、果肉中ATP、ADP含量和能荷,提高了H^(+)-ATPase、Ca^(2+)-ATPase、琥珀酸脱氢酶(succinate dehydrogenase,SDH)和细胞色素c氧化酶(cytochrome c oxidase,CCO)的活力,并诱导PaH^(+)-ATPase1、PaCa^(2+)-ATPase10、PaSDH和PaCCO6基因表达。果皮和果肉中叶绿素含量与ATP含量、ADP含量、能荷水平均呈正相关,与类胡萝卜素含量均呈负相关;其中,叶绿素含量与ADP含量在果皮、果肉中均呈极显著正相关(P<0.01)。说明外源CaCl_(2)可通过调节能量代谢延缓小白杏转色,保持较高的能荷,从而延长小白杏采后货架期。展开更多
Propylene glycol-based MWCNT(multi-walled carbon nanotubes)nanofluids were prepared in the framework of a two-step method and by using a suitable PVP(polyvinyl pyrrolidone)dispersant.The BBD(Box-Behnken design)model wa...Propylene glycol-based MWCNT(multi-walled carbon nanotubes)nanofluids were prepared in the framework of a two-step method and by using a suitable PVP(polyvinyl pyrrolidone)dispersant.The BBD(Box-Behnken design)model was exploited to analyze 17 sets of experiments and examine the sensitivity of the absorbance to three parameters,namely the concentration of MWCNT,the SN ratio(mass ratio of carbon nanotubes to sur-factants)and the sonication time.The results have revealed that,while the SN ratio and concentration of MWCNT have a strong effect on the absorbance,the influence of the sonication time is less important.The sta-tistical method of analysis of variance(ANOVA)was further used to determine the F-and p-values of the model.Five experiments were run to validate this approach.Since sample 2 was found to display the greatest absorbance,it was selected for stability monitoring as well as thermal conductivity and viscosity measurements.This sample has been found to be stable;the viscosity decreased with increasing temperature;the addition of MWCNT nano-particles was more effective in improving the thermal conductivity of propylene glycol than other methods in the literature.Moreover,the MWCNT nanofluid based on propylene glycol exhibited higher thermal conductivity at low temperatures.展开更多
In this study,comparing multiple models of machine learning,a multiple linear regression(MLP),multilayer feed-forward artificial neural network(BP)model,and a radial-basis feed-forward artificial neural network(RBF-BP...In this study,comparing multiple models of machine learning,a multiple linear regression(MLP),multilayer feed-forward artificial neural network(BP)model,and a radial-basis feed-forward artificial neural network(RBF-BP)model are selected for the optimization of the thermal properties of TiO_(2)/water nanofluids.In particular,the least squares support vector machine(LS-SVM)method and radial basis support vector machine(RB-SVM)method are implemented.First,curve fitting is performed by means of multiple linear regression in order to obtain bivariate correlation functions for thermal conductivity and viscosity of the nanofluid.Then the aforementioned models are used for a predictive analysis of the dependence of its thermal conductivity and viscosity on temperature and volume fraction.The results show that the least squares support vector machine(LS-SVM)has a prediction accuracy higher than the other models.The model predicts the thermal conductivity of TiO_(2)/water MSE=1.0853×10^(-6),R2=0.99864,MAE=0.00092,RMSE=0.00104,and the viscosity of TiO_(2)/water MSE=8.1397×10^(-6),R2=0.99995,MAE=0.00074,RMSE=0.0009.展开更多
目的探讨碘乙酸钠(monosodium iodoacetate,MIA)大鼠骨关节炎(osteoarthritis,OA)模型在不同时段的病理及软骨下骨微结构的变化特点。方法选取SPF级8周龄雄性Wistar大鼠36只,通过随机数字法分成空白对照组(Sham组)、OA造模2周组(OA-2W组...目的探讨碘乙酸钠(monosodium iodoacetate,MIA)大鼠骨关节炎(osteoarthritis,OA)模型在不同时段的病理及软骨下骨微结构的变化特点。方法选取SPF级8周龄雄性Wistar大鼠36只,通过随机数字法分成空白对照组(Sham组)、OA造模2周组(OA-2W组)、OA造模4周组(OA-4W组),分别进行MIA造模2周和4周后,获取膝关节胫骨标本并行组织切片染色及Micro-CT扫描,计算软骨组织OOCHAS评分及软骨下骨骨微结构参数。结果MIA造模后可见软骨表面缺损,关节周缘骨赘增生,软骨下骨密度(bone mineral density,BMD)减低,骨小梁变薄稀疏,组织切片见软骨结构形态紊乱,局部簇集现象,染色变浅甚至消失。Sham组软骨下BMD平均为(1367.97±37.43)mg/cc,OA-2W组BMD下降至(1330.66±38.99)mg/cc,OA-4W组BMD下降至(1285.5±47.08)mg/cc。软骨组织切片OOCHAS评分,Sham组平均为0(0,1)分,OA-2W组平均为12(9.75,15)分,OA-4W组平均为24(20,24)分。结论MIA造模2周时达到OA中期阶段,造模4周时为OA晚期阶段,OA会造成软骨下骨密度减低、骨小梁稀疏等骨质疏松表现,并且随着病情进展会逐渐加重。展开更多
文摘以新疆小白杏为试材,采用3 g/100 mL CaCl_(2)或蒸馏水(CK组)雾化熏蒸处理,测定小白杏在(10±1.0)℃、相对湿度(80±5)%条件下果皮、果肉中叶绿素、类胡萝卜素、腺苷三磷酸(adenosine triphosphate,ATP)、二磷酸腺苷(adenosine diphosphate,ADP)、单磷酸腺苷(adenosine monophosphate,AMP)含量和能荷的变化,分析能量代谢相关酶活力及相关基因的表达量变化,探究外源CaCl_(2)处理对小白杏采后能量代谢的调控机制,明确能量代谢水平与转色之间的联系。结果表明,与CK组相比,CaCl_(2)处理小白杏可延缓果皮、果肉中叶绿素降解,抑制类胡萝卜的合成,维持果皮、果肉中ATP、ADP含量和能荷,提高了H^(+)-ATPase、Ca^(2+)-ATPase、琥珀酸脱氢酶(succinate dehydrogenase,SDH)和细胞色素c氧化酶(cytochrome c oxidase,CCO)的活力,并诱导PaH^(+)-ATPase1、PaCa^(2+)-ATPase10、PaSDH和PaCCO6基因表达。果皮和果肉中叶绿素含量与ATP含量、ADP含量、能荷水平均呈正相关,与类胡萝卜素含量均呈负相关;其中,叶绿素含量与ADP含量在果皮、果肉中均呈极显著正相关(P<0.01)。说明外源CaCl_(2)可通过调节能量代谢延缓小白杏转色,保持较高的能荷,从而延长小白杏采后货架期。
基金This research is financially supported by the National Natural Science Foundation of China under Contract(No.51966005).
文摘Propylene glycol-based MWCNT(multi-walled carbon nanotubes)nanofluids were prepared in the framework of a two-step method and by using a suitable PVP(polyvinyl pyrrolidone)dispersant.The BBD(Box-Behnken design)model was exploited to analyze 17 sets of experiments and examine the sensitivity of the absorbance to three parameters,namely the concentration of MWCNT,the SN ratio(mass ratio of carbon nanotubes to sur-factants)and the sonication time.The results have revealed that,while the SN ratio and concentration of MWCNT have a strong effect on the absorbance,the influence of the sonication time is less important.The sta-tistical method of analysis of variance(ANOVA)was further used to determine the F-and p-values of the model.Five experiments were run to validate this approach.Since sample 2 was found to display the greatest absorbance,it was selected for stability monitoring as well as thermal conductivity and viscosity measurements.This sample has been found to be stable;the viscosity decreased with increasing temperature;the addition of MWCNT nano-particles was more effective in improving the thermal conductivity of propylene glycol than other methods in the literature.Moreover,the MWCNT nanofluid based on propylene glycol exhibited higher thermal conductivity at low temperatures.
基金supported by the National Natural Science Foundation of China(Nos.51966005,51866003)Yunnan Basic Research Program Project(2019FB071).
文摘In this study,comparing multiple models of machine learning,a multiple linear regression(MLP),multilayer feed-forward artificial neural network(BP)model,and a radial-basis feed-forward artificial neural network(RBF-BP)model are selected for the optimization of the thermal properties of TiO_(2)/water nanofluids.In particular,the least squares support vector machine(LS-SVM)method and radial basis support vector machine(RB-SVM)method are implemented.First,curve fitting is performed by means of multiple linear regression in order to obtain bivariate correlation functions for thermal conductivity and viscosity of the nanofluid.Then the aforementioned models are used for a predictive analysis of the dependence of its thermal conductivity and viscosity on temperature and volume fraction.The results show that the least squares support vector machine(LS-SVM)has a prediction accuracy higher than the other models.The model predicts the thermal conductivity of TiO_(2)/water MSE=1.0853×10^(-6),R2=0.99864,MAE=0.00092,RMSE=0.00104,and the viscosity of TiO_(2)/water MSE=8.1397×10^(-6),R2=0.99995,MAE=0.00074,RMSE=0.0009.
文摘目的探讨碘乙酸钠(monosodium iodoacetate,MIA)大鼠骨关节炎(osteoarthritis,OA)模型在不同时段的病理及软骨下骨微结构的变化特点。方法选取SPF级8周龄雄性Wistar大鼠36只,通过随机数字法分成空白对照组(Sham组)、OA造模2周组(OA-2W组)、OA造模4周组(OA-4W组),分别进行MIA造模2周和4周后,获取膝关节胫骨标本并行组织切片染色及Micro-CT扫描,计算软骨组织OOCHAS评分及软骨下骨骨微结构参数。结果MIA造模后可见软骨表面缺损,关节周缘骨赘增生,软骨下骨密度(bone mineral density,BMD)减低,骨小梁变薄稀疏,组织切片见软骨结构形态紊乱,局部簇集现象,染色变浅甚至消失。Sham组软骨下BMD平均为(1367.97±37.43)mg/cc,OA-2W组BMD下降至(1330.66±38.99)mg/cc,OA-4W组BMD下降至(1285.5±47.08)mg/cc。软骨组织切片OOCHAS评分,Sham组平均为0(0,1)分,OA-2W组平均为12(9.75,15)分,OA-4W组平均为24(20,24)分。结论MIA造模2周时达到OA中期阶段,造模4周时为OA晚期阶段,OA会造成软骨下骨密度减低、骨小梁稀疏等骨质疏松表现,并且随着病情进展会逐渐加重。
