Objective To review published literatures comparing the safety and effectiveness of retroperitoneal laparoscopic partial nephrectomy(RLPN) with transperitoneal laparoscopic partial nephrectomy(TLPN) and provide refere...Objective To review published literatures comparing the safety and effectiveness of retroperitoneal laparoscopic partial nephrectomy(RLPN) with transperitoneal laparoscopic partial nephrectomy(TLPN) and provide reference for clinical work. Methods The search strategy was performed to identify relevant papers from the Cochrane Central Register of Controlled Trials, EMBASE, MEDLINE, Google Scholar, China Hospital Knowledge Database, Wangfang Chinese Periodical Database, and VIP Chinese Periodical Database. All papers comparing RLPN with TLPN were included from 2000 to 2015. Two to three reviewers independently screened, evaluated, and extracted the included papers. A Meta-analysis was executed by using Review Manager 5.3 software. The interesting outcomes were tumor size, operating time, estimated blood loss, warm ischaemia time, length of hospital stay, positive margin rate, open conversion rate, overall complication rate, and recurrence rate. Results The literature search obtained 378 papers, then 10 of them were ultimately met the inclusion criteria and included in the systematic review. Finally, 6 of the 10 papers were included in the Meta-analysis. RLPN had significantly less operating time [P = 0.01, mean difference(MD)=-33.68, 95% confidence interval(CI) within(-60.35,-7.01)] and shorter length of hospital stay [P < 0.0001, MD=-1.47, 95% CI within(-2.18,-0.76)] than TLPN. Significant differences were not found between RLPN and TLPN in other outcomes. Conclusions RLPN may be equally safe and be faster than TLPN. Each center can choose a modality according to your own operating habits and experience.展开更多
Geological reports are a significant accomplishment for geologists involved in geological investigations and scientific research as they contain rich data and textual information.With the rapid development of science ...Geological reports are a significant accomplishment for geologists involved in geological investigations and scientific research as they contain rich data and textual information.With the rapid development of science and technology,a large number of textual reports have accumulated in the field of geology.However,many non-hot topics and non-English speaking regions are neglected in mainstream geoscience databases for geological information mining,making it more challenging for some researchers to extract necessary information from these texts.Natural Language Processing(NLP)has obvious advantages in processing large amounts of textual data.The objective of this paper is to identify geological named entities from Chinese geological texts using NLP techniques.We propose the RoBERTa-Prompt-Tuning-NER method,which leverages the concept of Prompt Learning and requires only a small amount of annotated data to train superior models for recognizing geological named entities in low-resource dataset configurations.The RoBERTa layer captures context-based information and longer-distance dependencies through dynamic word vectors.Finally,we conducted experiments on the constructed Geological Named Entity Recognition(GNER)dataset.Our experimental results show that the proposed model achieves the highest F1 score of 80.64%among the four baseline algorithms,demonstrating the reliability and robustness of using the model for Named Entity Recognition of geological texts.展开更多
To evaluate the role of nitric oxide in the biological effects of vascular endothelial growth factor(VEGF)and the possible mechanism of VEGF,the in vitro cultured vascular endothelial cells of rabbit aorta were divide...To evaluate the role of nitric oxide in the biological effects of vascular endothelial growth factor(VEGF)and the possible mechanism of VEGF,the in vitro cultured vascular endothelial cells of rabbit aorta were divided into control group,VEGF-treated group and VEGF+N-nitro-L-arginine methyl ester(L-NAME)-treated group.The absorbance(A)value of vascular endothelial cells and the levels of prostaglandin(PGI2),endothelin-1(ET-1)and von Willebrand factor(vWF)in the supernatant were observed by water-soluble tetrazo-lium salt assay,radioimmunoassay and enzyme-linked immunosorbent assay.The A values and PGI2 in VEGF-treated group and VEGF+L-NAME-treated group were higher than those in control group(P<0.05 and P<0.01).The ET-1 and vWF were significantly decreased in VEGF-treated group and VEGF+L-NAME-treated group com-pared with the control(P<0.05 and P<0.01).These results indicate that VEGF could promote the proliferation and secretion of PGI2 and inhibit the secretion of ET-1 and vWF in vascular endothelial cells and that L-NAME could inhibit the effect of VEGF partially.Nitric oxide is an important mediator in the process of stimulating prolifera-tion and regulating secretion of vascular endothelial cells by VEGF.展开更多
To evaluate the mechanism of vascular endothelial growth factor(VEGF)on the prevention of restenosis after angioplasty,the recombinant adenovirus vector containing hVEGF165 cDNA was constructed and transfected into va...To evaluate the mechanism of vascular endothelial growth factor(VEGF)on the prevention of restenosis after angioplasty,the recombinant adenovirus vector containing hVEGF165 cDNA was constructed and transfected into vascular smooth muscle cells(VSMC)in vitro.The conditioned medium containing VEGF was collected 72 h after the infection.Then,the VSMC and human umbilical vein endothelial cells(HUVEC)were divided into control group,H2O2-treated group and H2O2+VEGF-treated group to observe the proliferation and apoptosis by water soluble tetrazolium(WST-1)method,in situ nick end labeling(TUNEL)andflow cytometry(FCM).Compared with the control and H2O2+VEGF-treated groups,the absorbance(A)value of HUVEC was decreased,and apoptosis of HUVEC was significantly increased in H2O2-treated group.The changes of A value and apoptosis of VSMC were contrary to those of HUVEC.H2O2 could stimulate the proliferation of VSMC and induce the apoptosis of HUVEC,inhibit the proliferation of HUVEC and the apoptosis of VSMC and induce restenosis.VEGF could inhibit the effect of H2O2 on HUVEC and VSMC and prevent restenosis.These results offered further theoretical evidence for VEGF on the prevention of restenosis after angioplasty.展开更多
基金Supported by the Science and Technology Plans of Shaoxing Science and Technology Bureau,China(2010D10014)
文摘Objective To review published literatures comparing the safety and effectiveness of retroperitoneal laparoscopic partial nephrectomy(RLPN) with transperitoneal laparoscopic partial nephrectomy(TLPN) and provide reference for clinical work. Methods The search strategy was performed to identify relevant papers from the Cochrane Central Register of Controlled Trials, EMBASE, MEDLINE, Google Scholar, China Hospital Knowledge Database, Wangfang Chinese Periodical Database, and VIP Chinese Periodical Database. All papers comparing RLPN with TLPN were included from 2000 to 2015. Two to three reviewers independently screened, evaluated, and extracted the included papers. A Meta-analysis was executed by using Review Manager 5.3 software. The interesting outcomes were tumor size, operating time, estimated blood loss, warm ischaemia time, length of hospital stay, positive margin rate, open conversion rate, overall complication rate, and recurrence rate. Results The literature search obtained 378 papers, then 10 of them were ultimately met the inclusion criteria and included in the systematic review. Finally, 6 of the 10 papers were included in the Meta-analysis. RLPN had significantly less operating time [P = 0.01, mean difference(MD)=-33.68, 95% confidence interval(CI) within(-60.35,-7.01)] and shorter length of hospital stay [P < 0.0001, MD=-1.47, 95% CI within(-2.18,-0.76)] than TLPN. Significant differences were not found between RLPN and TLPN in other outcomes. Conclusions RLPN may be equally safe and be faster than TLPN. Each center can choose a modality according to your own operating habits and experience.
基金supported by the National Natural Science Foundation of China(Nos.42488201,42172137,42050104,and 42050102)the National Key R&D Program of China(No.2023YFF0804000)+1 种基金Sichuan Provincial Youth Science&Technology Innovative Research Group Fund(No.2022JDTD0004)This study is a contribution to the Deep-time Digital Earth(DDE)Big Science Program and IGCP 739.
文摘Geological reports are a significant accomplishment for geologists involved in geological investigations and scientific research as they contain rich data and textual information.With the rapid development of science and technology,a large number of textual reports have accumulated in the field of geology.However,many non-hot topics and non-English speaking regions are neglected in mainstream geoscience databases for geological information mining,making it more challenging for some researchers to extract necessary information from these texts.Natural Language Processing(NLP)has obvious advantages in processing large amounts of textual data.The objective of this paper is to identify geological named entities from Chinese geological texts using NLP techniques.We propose the RoBERTa-Prompt-Tuning-NER method,which leverages the concept of Prompt Learning and requires only a small amount of annotated data to train superior models for recognizing geological named entities in low-resource dataset configurations.The RoBERTa layer captures context-based information and longer-distance dependencies through dynamic word vectors.Finally,we conducted experiments on the constructed Geological Named Entity Recognition(GNER)dataset.Our experimental results show that the proposed model achieves the highest F1 score of 80.64%among the four baseline algorithms,demonstrating the reliability and robustness of using the model for Named Entity Recognition of geological texts.
基金supported by a grant from the Natural Science Foundation of Hubei Province(No.2003ABA135).
文摘To evaluate the role of nitric oxide in the biological effects of vascular endothelial growth factor(VEGF)and the possible mechanism of VEGF,the in vitro cultured vascular endothelial cells of rabbit aorta were divided into control group,VEGF-treated group and VEGF+N-nitro-L-arginine methyl ester(L-NAME)-treated group.The absorbance(A)value of vascular endothelial cells and the levels of prostaglandin(PGI2),endothelin-1(ET-1)and von Willebrand factor(vWF)in the supernatant were observed by water-soluble tetrazo-lium salt assay,radioimmunoassay and enzyme-linked immunosorbent assay.The A values and PGI2 in VEGF-treated group and VEGF+L-NAME-treated group were higher than those in control group(P<0.05 and P<0.01).The ET-1 and vWF were significantly decreased in VEGF-treated group and VEGF+L-NAME-treated group com-pared with the control(P<0.05 and P<0.01).These results indicate that VEGF could promote the proliferation and secretion of PGI2 and inhibit the secretion of ET-1 and vWF in vascular endothelial cells and that L-NAME could inhibit the effect of VEGF partially.Nitric oxide is an important mediator in the process of stimulating prolifera-tion and regulating secretion of vascular endothelial cells by VEGF.
基金supported by the Natural Science Foundation of Hubei Province of China(No.2003ABA135).
文摘To evaluate the mechanism of vascular endothelial growth factor(VEGF)on the prevention of restenosis after angioplasty,the recombinant adenovirus vector containing hVEGF165 cDNA was constructed and transfected into vascular smooth muscle cells(VSMC)in vitro.The conditioned medium containing VEGF was collected 72 h after the infection.Then,the VSMC and human umbilical vein endothelial cells(HUVEC)were divided into control group,H2O2-treated group and H2O2+VEGF-treated group to observe the proliferation and apoptosis by water soluble tetrazolium(WST-1)method,in situ nick end labeling(TUNEL)andflow cytometry(FCM).Compared with the control and H2O2+VEGF-treated groups,the absorbance(A)value of HUVEC was decreased,and apoptosis of HUVEC was significantly increased in H2O2-treated group.The changes of A value and apoptosis of VSMC were contrary to those of HUVEC.H2O2 could stimulate the proliferation of VSMC and induce the apoptosis of HUVEC,inhibit the proliferation of HUVEC and the apoptosis of VSMC and induce restenosis.VEGF could inhibit the effect of H2O2 on HUVEC and VSMC and prevent restenosis.These results offered further theoretical evidence for VEGF on the prevention of restenosis after angioplasty.
