BACKGROUND Knee joint pain and stiffness are the two main symptoms of knee osteoarthritis(OA)and thus restrict a patient’s activities,such as walking and walking up and downstairs.The lower body positive pressure(LBP...BACKGROUND Knee joint pain and stiffness are the two main symptoms of knee osteoarthritis(OA)and thus restrict a patient’s activities,such as walking and walking up and downstairs.The lower body positive pressure(LBPP)treadmill as one of the emerging body weight support system devices brings new hope for exerciserelated rehabilitation for knee OA patients.AIM To investigate the biomechanical effects and the subjective clinical assessment of LBPP treadmill walking exercise when compared with conventional therapy in mild to moderate knee OA patients.METHODS Eighteen patients with mild-to-moderate knee OA were recruited in this randomized controlled trial(RCT)study.The eligible knee OA patients were randomly assigned to two groups:LBPP and control groups.The patients in the LBPP group performed an LBPP walking training program for 30 min/session per day,6 d per week for 2 wk whereas the patients in the control group performed walking on the ground for the same amount.All patients underwent clinical assessments and three-dimensional gait analysis at pre-and 2-wk post-treatment.RESULTS The Western Ontario and McMaster Universities Arthritis Index and visual analog scale scores in both the LBPP group and control group were found to decrease significantly at the post-treatment point than the pre-treatment point(LBPP:70.25±13.93 vs 40.50±11.86;3.88±0.99 vs 1.63±0.52;control:69.20±8.88 vs 48.10±8.67;3.80±0.79 vs 2.60±0.70,P<0.001).Moreover,compared with the control group,the LBPP group showed more improvements in walking speed(P=0.007),stride length(P=0.037),and knee range of motion(P=0.048)during walking,which represented more improvement in walking ability.CONCLUSION The results of our RCT study showed that the LBPP group has a greater effect on improving gait parameters than the conventional group,although there was no significant advantage in clinical assessment.This finding indicates that LBPP treadmill walking training might be an effective approach for alleviating pain symptoms and improving lower extremity locomotion in mild to moderate knee OA patients.展开更多
Objective:This study aimed to observe the effects of acupuncture on iron metabolism in the hippocampus of rats with cerebral ischemia-reperfusion injury(CIRI)and to explore the mechanism of acupuncture on neural repai...Objective:This study aimed to observe the effects of acupuncture on iron metabolism in the hippocampus of rats with cerebral ischemia-reperfusion injury(CIRI)and to explore the mechanism of acupuncture on neural repairinCIRIrats.Methods:After general feeding for 3 days,16 of 48 healthy Sprague-Dawley rats were selected according to a random number table to form a sham-occlusion group,and the models were prepared in the remaining rats.After successful modeling,the rats were randomized into model and acupuncture groups,with 16 rats in each group.The CIRI models were prepared using the modified Zea Longa suture-occlusion method.In the acupuncture group,the rats were fixed and acupuncture was delivered at“Shuigou"(GV26),“Dazhui"(GV14),and“Baihui"(GV20),and the needles were manipulated once every 15 min.In the sham-occlusion and model groups,rats were fixed without acupuncture.The intervention was performed for 30 min in each group,once every 12 h,seven times.The degree of neurological impairment was assessed by using the modified Garcia score.Cerebral blood flow(CBF)was monitored using a laser speckle blood flow imaging system.The cerebral infarction area ratio was measured using triphenyltetrazolium chloride staining.Ferric ion aggregation in brain tissue was noted using Prussian blue staining.The ferric ion and glutathione(GSH)contents of the hippocampal tissue on the ischemic side were detected using ferric ion and GSH kits.The protein expression of transferrin receptor 1(TFR1),iron regulatory protein 2(IRP2),and ferroportin(FPN)was detected via western blotting.Results:(1)Before intervention,compared with the sham-occlusion group,the Garcia neurological score was significantly reduced in the model and acupuncture groups(both P<0.01).After the intervention,compared with the sham-occlusion group,the Garcia neurological score in the model group decreased significantly(P<0.01),and the score in the acupuncture group was elevated compared with the model group(P<0.o5).Compared to before the intervention,the Garcia neurological score increased after the intervention in the acupuncture group(P<0.05).(2)Compared with the sham-occlusion group,in the model group,CBF was reduced,the infarct area was significantly enlarged,ferric ion aggregation increased in the hippocampus,the content of ferric ion increased,and that of GSH decreased significantly(both P<0.01),while the protein expression of TFR1 and IRP2 was upregulated and that of FPN was downregulated(both P<0.05).When compared with the model group,in the acupuncture group,CBF was increased,the infarct area was reduced(P<0.01),the ferric ion aggregation was reduced in the hippocampus,the content of ferric ion decreased,and that of GSH increased significantly(P<0.05,P<0.01);the protein expression of TFR1 and IRP2 was downregulated and that of FPN was upregulated(both P<0.05).Conclusion:Acupuncture can alleviate CiRI,which may be related to the regulation of iron homeostasisassociated transferrin in the hippocampus,attenuation of ferric ion aggregation in hippocampal tissue,reduction of lipid peroxidation,and inhibition of ferroptosis.展开更多
Background: In our previous study, we identified a candidate tumor suppressor gene, testin LIM domain protein (TES), in primary gastric cancer (GC). TES contains three LIM domains, which are specific interacting regio...Background: In our previous study, we identified a candidate tumor suppressor gene, testin LIM domain protein (TES), in primary gastric cancer (GC). TES contains three LIM domains, which are specific interacting regions for the cell adhesion and cytoskeleton regulatory proteins. Mena is a known cytoskeleton regulator that regulates the assembly of actin filaments and modulates cell adhesion and motility by interacting with Lamellipodin (Lpd). Therefore, we hypothesized that TES plays a role as tumor suppressor in GC through interacting with Mena. This study aimed to investigate the tumor suppressive functions of TES in GC. Methods: We explored the tumor suppressive effect of TES in GC by in vitro cell proliferation assay, colony formation assay, cell cycle analysis, Transwell assays, and in vivo tumorigenicity and metastasis assays. The interaction of TES and Mena was investigated through immunoprecipitation-based mass spectrometry. We also analyzed the expression of TES and Mena in 172 GC specimens using immunohistochemistry and investigated the clinicopathological and prog-nostic significance of TES and Mena in GC. Results: TES suppressed GC cell proliferation and colony formation, induced cell cycle arrest, and inhibited tumorigenicity in vitro. Additionally, it inhibited GC cell migration and invasion in vitro and suppressed metastasis in vivo. TES interacted with Mena, and inhibited the interaction of Mena with Lpd. Transwell assays suggested that TES suppressed migration and invasion of GC cells in a Mena-dependent fashion. In GC patients with high Mena expression, the expression of TES was associated with tumor infiltration (P = 0.005), lymph node metastasis (P = 0.003), TNM stage (P = 0.003), and prognosis (P = 0.010). However, no significant association was observed in GC patients with low Mena expression. Conclusions: We believe that TES functions as a Mena-dependent tumor suppressor. TES represents a valuable prog-nostic marker and potential target for GC treatment.展开更多
基金the Medical Ethics Association of the Fifth Affiliated Hospital of Guangzhou Medical University(No.KY01-2018-10-18).
文摘BACKGROUND Knee joint pain and stiffness are the two main symptoms of knee osteoarthritis(OA)and thus restrict a patient’s activities,such as walking and walking up and downstairs.The lower body positive pressure(LBPP)treadmill as one of the emerging body weight support system devices brings new hope for exerciserelated rehabilitation for knee OA patients.AIM To investigate the biomechanical effects and the subjective clinical assessment of LBPP treadmill walking exercise when compared with conventional therapy in mild to moderate knee OA patients.METHODS Eighteen patients with mild-to-moderate knee OA were recruited in this randomized controlled trial(RCT)study.The eligible knee OA patients were randomly assigned to two groups:LBPP and control groups.The patients in the LBPP group performed an LBPP walking training program for 30 min/session per day,6 d per week for 2 wk whereas the patients in the control group performed walking on the ground for the same amount.All patients underwent clinical assessments and three-dimensional gait analysis at pre-and 2-wk post-treatment.RESULTS The Western Ontario and McMaster Universities Arthritis Index and visual analog scale scores in both the LBPP group and control group were found to decrease significantly at the post-treatment point than the pre-treatment point(LBPP:70.25±13.93 vs 40.50±11.86;3.88±0.99 vs 1.63±0.52;control:69.20±8.88 vs 48.10±8.67;3.80±0.79 vs 2.60±0.70,P<0.001).Moreover,compared with the control group,the LBPP group showed more improvements in walking speed(P=0.007),stride length(P=0.037),and knee range of motion(P=0.048)during walking,which represented more improvement in walking ability.CONCLUSION The results of our RCT study showed that the LBPP group has a greater effect on improving gait parameters than the conventional group,although there was no significant advantage in clinical assessment.This finding indicates that LBPP treadmill walking training might be an effective approach for alleviating pain symptoms and improving lower extremity locomotion in mild to moderate knee OA patients.
基金Supported by the Project of National Natural Science Fund of China:81874508,82274662the Project of Natural Science Fund of Hunan Province:2021J304905the Project of Natural Science Fund of Changsha Science and Technology Ministry of Hunan Province:kq2014094.
文摘Objective:This study aimed to observe the effects of acupuncture on iron metabolism in the hippocampus of rats with cerebral ischemia-reperfusion injury(CIRI)and to explore the mechanism of acupuncture on neural repairinCIRIrats.Methods:After general feeding for 3 days,16 of 48 healthy Sprague-Dawley rats were selected according to a random number table to form a sham-occlusion group,and the models were prepared in the remaining rats.After successful modeling,the rats were randomized into model and acupuncture groups,with 16 rats in each group.The CIRI models were prepared using the modified Zea Longa suture-occlusion method.In the acupuncture group,the rats were fixed and acupuncture was delivered at“Shuigou"(GV26),“Dazhui"(GV14),and“Baihui"(GV20),and the needles were manipulated once every 15 min.In the sham-occlusion and model groups,rats were fixed without acupuncture.The intervention was performed for 30 min in each group,once every 12 h,seven times.The degree of neurological impairment was assessed by using the modified Garcia score.Cerebral blood flow(CBF)was monitored using a laser speckle blood flow imaging system.The cerebral infarction area ratio was measured using triphenyltetrazolium chloride staining.Ferric ion aggregation in brain tissue was noted using Prussian blue staining.The ferric ion and glutathione(GSH)contents of the hippocampal tissue on the ischemic side were detected using ferric ion and GSH kits.The protein expression of transferrin receptor 1(TFR1),iron regulatory protein 2(IRP2),and ferroportin(FPN)was detected via western blotting.Results:(1)Before intervention,compared with the sham-occlusion group,the Garcia neurological score was significantly reduced in the model and acupuncture groups(both P<0.01).After the intervention,compared with the sham-occlusion group,the Garcia neurological score in the model group decreased significantly(P<0.01),and the score in the acupuncture group was elevated compared with the model group(P<0.o5).Compared to before the intervention,the Garcia neurological score increased after the intervention in the acupuncture group(P<0.05).(2)Compared with the sham-occlusion group,in the model group,CBF was reduced,the infarct area was significantly enlarged,ferric ion aggregation increased in the hippocampus,the content of ferric ion increased,and that of GSH decreased significantly(both P<0.01),while the protein expression of TFR1 and IRP2 was upregulated and that of FPN was downregulated(both P<0.05).When compared with the model group,in the acupuncture group,CBF was increased,the infarct area was reduced(P<0.01),the ferric ion aggregation was reduced in the hippocampus,the content of ferric ion decreased,and that of GSH increased significantly(P<0.05,P<0.01);the protein expression of TFR1 and IRP2 was downregulated and that of FPN was upregulated(both P<0.05).Conclusion:Acupuncture can alleviate CiRI,which may be related to the regulation of iron homeostasisassociated transferrin in the hippocampus,attenuation of ferric ion aggregation in hippocampal tissue,reduction of lipid peroxidation,and inhibition of ferroptosis.
基金This work was supported by the National Natural Science Foundation of China[Grant Numbers 81572865,31501132,81773110,81402281 and 81402560]Guangdong Province Science and Technology Plan Project[Grant Number 2012A030400059]+1 种基金The Innovation Project of Shandong Academy of Medical Sciences,the Shandong Key Research and Development Plan[Grant Number 2016GSF202042]The Distinguished Experts of Taishan Scholar Project[Grant Number ts201511074].
文摘Background: In our previous study, we identified a candidate tumor suppressor gene, testin LIM domain protein (TES), in primary gastric cancer (GC). TES contains three LIM domains, which are specific interacting regions for the cell adhesion and cytoskeleton regulatory proteins. Mena is a known cytoskeleton regulator that regulates the assembly of actin filaments and modulates cell adhesion and motility by interacting with Lamellipodin (Lpd). Therefore, we hypothesized that TES plays a role as tumor suppressor in GC through interacting with Mena. This study aimed to investigate the tumor suppressive functions of TES in GC. Methods: We explored the tumor suppressive effect of TES in GC by in vitro cell proliferation assay, colony formation assay, cell cycle analysis, Transwell assays, and in vivo tumorigenicity and metastasis assays. The interaction of TES and Mena was investigated through immunoprecipitation-based mass spectrometry. We also analyzed the expression of TES and Mena in 172 GC specimens using immunohistochemistry and investigated the clinicopathological and prog-nostic significance of TES and Mena in GC. Results: TES suppressed GC cell proliferation and colony formation, induced cell cycle arrest, and inhibited tumorigenicity in vitro. Additionally, it inhibited GC cell migration and invasion in vitro and suppressed metastasis in vivo. TES interacted with Mena, and inhibited the interaction of Mena with Lpd. Transwell assays suggested that TES suppressed migration and invasion of GC cells in a Mena-dependent fashion. In GC patients with high Mena expression, the expression of TES was associated with tumor infiltration (P = 0.005), lymph node metastasis (P = 0.003), TNM stage (P = 0.003), and prognosis (P = 0.010). However, no significant association was observed in GC patients with low Mena expression. Conclusions: We believe that TES functions as a Mena-dependent tumor suppressor. TES represents a valuable prog-nostic marker and potential target for GC treatment.
