Objective:Most of the studies on the herb Chuanxiong Rhizoma(CR)have focused on the L-arginine-nitric oxide(NO)pathway,but the nitrate-nitrite-NO(NO_(3)^(-)–NO_(2)^(-)–NO)pathway was rarely investigated.Therefore,th...Objective:Most of the studies on the herb Chuanxiong Rhizoma(CR)have focused on the L-arginine-nitric oxide(NO)pathway,but the nitrate-nitrite-NO(NO_(3)^(-)–NO_(2)^(-)–NO)pathway was rarely investigated.Therefore,the aim of this study was to evaluate the effects and mechanisms of action of CR in coronary artery disease(CAD).Methods:The NO_(3)^(-),NO_(2)^(-)and NO levels were examined in the NO_(3)^(-)–NO_(2)^(-)–NO pathway.High-performance ion chromatography was used to quantify NO_(3)^(-)and NO_(2)^(-)levels.Then,NO was quantified using a multifunctional enzyme marker with a fluorescent probe.The tension of aortic rings was measured using a multi myograph system.Results:High content of NO_(3)^(-)and low content of NO_(2)^(-)was found in CR,and which could potently convert NO_(3)^(-)to NO_(2)^(-)in the presence of endogenous reductase enzyme.Incubating human coronary artery endothelial cells(HCAECs)with CR-containing serum showed that CR significantly decreased the NO_(3)^(-)content and increased the levels of NO_(2)^(-)and NO in the cells under hypoxic conditions.In addition,CR significantly relaxed isolated aortic rings when the L-arginine–NO pathway was blocked.The optimal concentration of CR for relaxation was 200 mg/m L.Conclusion:CR supplements large amounts of NO in cells and vessels to achieve relaxation via the NO_(3)^(-)–NO_(2)^(-)–NO pathway,thereby making up for the deficiency caused by the lack of NO after the L-arginine-NO pathway is suppressed.This study also supports the potential use of a traditional Chinese herb for future drug development.展开更多
基金supported by the National Natural Science Foundation of China[grant No.81774115,2018]National Natural Science Foundation of China[grant No.81460721,2015]And Guangxi University of Traditional Chinese Medicine Key Master’s Research and Innovation Project of China[grant No.XYJ20080,2020]。
文摘Objective:Most of the studies on the herb Chuanxiong Rhizoma(CR)have focused on the L-arginine-nitric oxide(NO)pathway,but the nitrate-nitrite-NO(NO_(3)^(-)–NO_(2)^(-)–NO)pathway was rarely investigated.Therefore,the aim of this study was to evaluate the effects and mechanisms of action of CR in coronary artery disease(CAD).Methods:The NO_(3)^(-),NO_(2)^(-)and NO levels were examined in the NO_(3)^(-)–NO_(2)^(-)–NO pathway.High-performance ion chromatography was used to quantify NO_(3)^(-)and NO_(2)^(-)levels.Then,NO was quantified using a multifunctional enzyme marker with a fluorescent probe.The tension of aortic rings was measured using a multi myograph system.Results:High content of NO_(3)^(-)and low content of NO_(2)^(-)was found in CR,and which could potently convert NO_(3)^(-)to NO_(2)^(-)in the presence of endogenous reductase enzyme.Incubating human coronary artery endothelial cells(HCAECs)with CR-containing serum showed that CR significantly decreased the NO_(3)^(-)content and increased the levels of NO_(2)^(-)and NO in the cells under hypoxic conditions.In addition,CR significantly relaxed isolated aortic rings when the L-arginine–NO pathway was blocked.The optimal concentration of CR for relaxation was 200 mg/m L.Conclusion:CR supplements large amounts of NO in cells and vessels to achieve relaxation via the NO_(3)^(-)–NO_(2)^(-)–NO pathway,thereby making up for the deficiency caused by the lack of NO after the L-arginine-NO pathway is suppressed.This study also supports the potential use of a traditional Chinese herb for future drug development.
