Affinity membrane was used for the endotoxin adsorption. The matrix macropore membrane was prepared from filterpaper by chemically crosslinking, and polymyxin B was immobilized as affinity ligand. The prepared membran...Affinity membrane was used for the endotoxin adsorption. The matrix macropore membrane was prepared from filterpaper by chemically crosslinking, and polymyxin B was immobilized as affinity ligand. The prepared membrane cartridge can be used for the removal of endotoxin from aqueous solutions. With a cartridge of 40 sheets affinity membrane containing, the endotoxin level of the processed solution can be reduced to a minimum of 4 pg/mL.展开更多
A convenient derivatization method of amino acids with 1-fluoro-2,4-dinitrobezene as reaction reagent and a separation system were described. The derivative amino acids were separated on a specific chemically bonded p...A convenient derivatization method of amino acids with 1-fluoro-2,4-dinitrobezene as reaction reagent and a separation system were described. The derivative amino acids were separated on a specific chemically bonded phase column with a simple linear gradient elution consisting of aqueous buffer and methanol. The eluate was detected by common ultraviolet absorption detector at 360 nm. The detection limits of amino acids were as low as 10 picomole. This method has been successfully applied to assay amino acid injection liquor used in hospital. It has good reproducibility and precision. The procedures avoid the requirements of particular derivative equipment and analyzer employed in conventional amino acid analysis.展开更多
文摘Affinity membrane was used for the endotoxin adsorption. The matrix macropore membrane was prepared from filterpaper by chemically crosslinking, and polymyxin B was immobilized as affinity ligand. The prepared membrane cartridge can be used for the removal of endotoxin from aqueous solutions. With a cartridge of 40 sheets affinity membrane containing, the endotoxin level of the processed solution can be reduced to a minimum of 4 pg/mL.
基金supported by the National Natural Science Foundation of China.
文摘A convenient derivatization method of amino acids with 1-fluoro-2,4-dinitrobezene as reaction reagent and a separation system were described. The derivative amino acids were separated on a specific chemically bonded phase column with a simple linear gradient elution consisting of aqueous buffer and methanol. The eluate was detected by common ultraviolet absorption detector at 360 nm. The detection limits of amino acids were as low as 10 picomole. This method has been successfully applied to assay amino acid injection liquor used in hospital. It has good reproducibility and precision. The procedures avoid the requirements of particular derivative equipment and analyzer employed in conventional amino acid analysis.
