Background： The worsening of semen quality, due to the application of Wi-Fi, can be ameliorated by Vitamin E. This study aimed to demonstrate whether a moderate dose of trolox,a new Vitamin E,inhibits oxidative damag...Background： The worsening of semen quality, due to the application of Wi-Fi, can be ameliorated by Vitamin E. This study aimed to demonstrate whether a moderate dose of trolox,a new Vitamin E,inhibits oxidative damage on sperms in vitro after exposure to Wi-Fi radiation. Methods： Each of the twenty qualified semen, gathered from June to October 2014 in eugenics clinic, was separated into four aliquots, including sham, Wi-Fi-exposed, Wi-Fi plus 5 mmol/L trolox, and Wi-Fi plus 10 mmol/L trolox groups. At 0 min, all baseline parameters of the 20 samples were measured in sequence. Reactive oxygen species, glutathione, and superoxide dismutase were evaluated in the four aliquots at 45 and 90 min, as were sperm DNA fragments, sperm mitochondrial potential, relative amplification of sperm mitochondrial DNA, sperm vitality, and progressive and immotility sperm. The parameters were analyzed by one-way analysis of variance and Tukey＇s posttest. Results： Among Wi-Fi plus 5 mmol/L trolox, Wi-Fi-exposed and Wi-Fi plus 10 mmol/L trolox groups, reactive oxygen species levels （45 min： 3.80 ± 0.41 RLU·10^-6·ml^-1. 7.50 ± 0.35 RLU·10^-6·ml^-1vs. 6.70 ± 0.47 RLU·10^-6·ml^-1P 〈 0.001; 90 min：5.40 ± 0.21 RLU·10^-6·ml^-1vs. 10.10 ± 0.31 RLU·10^-6·ml^-1. 7.00 ± 0.42 RLU·10^-6·ml^-1P 〈 0.001, respectively）, percentages of tail DNA （45 min： 16.8 ± 2.0% vs. 31.9 ± 2.5% vs. 61.3 ± 1.6%, P 〈 0.001; 90 min： 19.7 ± 1.5% vs. 73.7 ± 1.3% vs. 73.1 ± 1.1%, P 〈 0.001, respectively）, 8-hydroxy-2＇-deoxyguanosine （45 min： 51.89 ± 1.46 pg/ml vs. 104.89 ± 2.19 pg/ml vs. 106.11 ± 1.81 pg/ml , P = 0.012;90 min： 79.96 ± 1.73 pg/ml vs. 141.73 ± 2.90 pg/ml vs. 139.06 ± 2.79 pg/ml; P 〈 0.001）, and percentages of immotility sperm （45 min：27.7 ± 2.7% vs. 41.7 ± 2.2% vs. 41.7 ± 2.5%; 90 min： 29.9 ± 3.3% vs. 58.9 ± 4.0% vs. 63.1 ± 4.0%; all P 〈 0.001） were lowest, and glutathione peroxidase （45 min： 60.50 ± 1.54 U/ml vs. 37.09 ± 1.77 U/ml vs. 28.18 ± 1.06 U/ml; 90 min： 44.61 ± 1.23 U/ml vs. 16.86 ± 0.93 U/ml vs. 29.94 ± 1.56 U/ml; all P 〈 0.001）, percentages of head DNA （45 min： 83.2 ± 2.0% vs. 68.2 ± 2.5% vs. 38.8 ± 1.6%; 90 min： 80.3 ± 1.5% vs. 26.3 ± 1.3% vs. 26.9 ± 1.1%; all P 〈 0.001）, percentages of sperm vitality （45 min： 89.5 ± 1.6% vs. 70.7 ± 3.1% vs. 57.7 ± 2.4%;90 min： 80.8 ± 2.2% vs. 40.4 ± 4.0% vs. 34.7 ± 3.9%; all P 〈 0.001）, and progressive sperm （45 min： 69.3 ± 2.7% vs. 55.8 ± 2.2% vs. 55.4 ± 2.5%; 90 min： 67.2 ± 3.3% vs. 38.2 ± 4.0% vs. 33.9 ± 4.0%; all P 〈 0.001） were highest in Wi-Fi plus 5 mmol/L trolox group at 45 and 90 min, respectively. Other parameters were not affected, while the sham group maintained the baseline. Conclusion：This study found that 5 mmol/L trolox protected the Wi-Fi-exposed semen in vitro from the damage of electromagnetic radiation-induced oxidative stress.展开更多
To the Editor:Premature ejaculation(PE)is one of the commonplace male sexual dysfunctions affecting about 30%of men worldwide.PE can either be lifelong or acquired.Genetic polymorphisms situated on the SLC6A4 of human...To the Editor:Premature ejaculation(PE)is one of the commonplace male sexual dysfunctions affecting about 30%of men worldwide.PE can either be lifelong or acquired.Genetic polymorphisms situated on the SLC6A4 of humans encoding the 5-hydroxytryptamine transporter(serotonin transporter)(5-HTT)also called the serotonin transporter(SERT),a significant controller of serotonergic neurotransmission,were related to the pathogenesis of PE.[1,2]Polymorphisms in SLC6A4 are associated with the incidence of lifelong premature ejaculation(LPR).The effects of 5-hydroxytryptamine(serotonin)transporter gene-linked polymorphic region(5-HTTLPR)and serotonin transporter gene intron 2(STin2)polymorphism on lifelong PE(LPE)are controversial.We aimed to determine possible relationships between 5-HTTLPR and STin2 polymorphisms in the SERT gene and clinical response of a selective serotonin reuptake inhibitor(dapoxetine)in LPE.展开更多
基金supported by the National Natural Science Foundation of China(No.81300541)the Technology Project of Guizhou Province(No.QKHJZ[2013]2051)the Doctoral Fund of the Affiliated Hospital of Guiyang Medical College(No.C-2012-6),China
文摘Background： The worsening of semen quality, due to the application of Wi-Fi, can be ameliorated by Vitamin E. This study aimed to demonstrate whether a moderate dose of trolox,a new Vitamin E,inhibits oxidative damage on sperms in vitro after exposure to Wi-Fi radiation. Methods： Each of the twenty qualified semen, gathered from June to October 2014 in eugenics clinic, was separated into four aliquots, including sham, Wi-Fi-exposed, Wi-Fi plus 5 mmol/L trolox, and Wi-Fi plus 10 mmol/L trolox groups. At 0 min, all baseline parameters of the 20 samples were measured in sequence. Reactive oxygen species, glutathione, and superoxide dismutase were evaluated in the four aliquots at 45 and 90 min, as were sperm DNA fragments, sperm mitochondrial potential, relative amplification of sperm mitochondrial DNA, sperm vitality, and progressive and immotility sperm. The parameters were analyzed by one-way analysis of variance and Tukey＇s posttest. Results： Among Wi-Fi plus 5 mmol/L trolox, Wi-Fi-exposed and Wi-Fi plus 10 mmol/L trolox groups, reactive oxygen species levels （45 min： 3.80 ± 0.41 RLU·10^-6·ml^-1. 7.50 ± 0.35 RLU·10^-6·ml^-1vs. 6.70 ± 0.47 RLU·10^-6·ml^-1P 〈 0.001; 90 min：5.40 ± 0.21 RLU·10^-6·ml^-1vs. 10.10 ± 0.31 RLU·10^-6·ml^-1. 7.00 ± 0.42 RLU·10^-6·ml^-1P 〈 0.001, respectively）, percentages of tail DNA （45 min： 16.8 ± 2.0% vs. 31.9 ± 2.5% vs. 61.3 ± 1.6%, P 〈 0.001; 90 min： 19.7 ± 1.5% vs. 73.7 ± 1.3% vs. 73.1 ± 1.1%, P 〈 0.001, respectively）, 8-hydroxy-2＇-deoxyguanosine （45 min： 51.89 ± 1.46 pg/ml vs. 104.89 ± 2.19 pg/ml vs. 106.11 ± 1.81 pg/ml , P = 0.012;90 min： 79.96 ± 1.73 pg/ml vs. 141.73 ± 2.90 pg/ml vs. 139.06 ± 2.79 pg/ml; P 〈 0.001）, and percentages of immotility sperm （45 min：27.7 ± 2.7% vs. 41.7 ± 2.2% vs. 41.7 ± 2.5%; 90 min： 29.9 ± 3.3% vs. 58.9 ± 4.0% vs. 63.1 ± 4.0%; all P 〈 0.001） were lowest, and glutathione peroxidase （45 min： 60.50 ± 1.54 U/ml vs. 37.09 ± 1.77 U/ml vs. 28.18 ± 1.06 U/ml; 90 min： 44.61 ± 1.23 U/ml vs. 16.86 ± 0.93 U/ml vs. 29.94 ± 1.56 U/ml; all P 〈 0.001）, percentages of head DNA （45 min： 83.2 ± 2.0% vs. 68.2 ± 2.5% vs. 38.8 ± 1.6%; 90 min： 80.3 ± 1.5% vs. 26.3 ± 1.3% vs. 26.9 ± 1.1%; all P 〈 0.001）, percentages of sperm vitality （45 min： 89.5 ± 1.6% vs. 70.7 ± 3.1% vs. 57.7 ± 2.4%;90 min： 80.8 ± 2.2% vs. 40.4 ± 4.0% vs. 34.7 ± 3.9%; all P 〈 0.001）, and progressive sperm （45 min： 69.3 ± 2.7% vs. 55.8 ± 2.2% vs. 55.4 ± 2.5%; 90 min： 67.2 ± 3.3% vs. 38.2 ± 4.0% vs. 33.9 ± 4.0%; all P 〈 0.001） were highest in Wi-Fi plus 5 mmol/L trolox group at 45 and 90 min, respectively. Other parameters were not affected, while the sham group maintained the baseline. Conclusion：This study found that 5 mmol/L trolox protected the Wi-Fi-exposed semen in vitro from the damage of electromagnetic radiation-induced oxidative stress.
文摘To the Editor:Premature ejaculation(PE)is one of the commonplace male sexual dysfunctions affecting about 30%of men worldwide.PE can either be lifelong or acquired.Genetic polymorphisms situated on the SLC6A4 of humans encoding the 5-hydroxytryptamine transporter(serotonin transporter)(5-HTT)also called the serotonin transporter(SERT),a significant controller of serotonergic neurotransmission,were related to the pathogenesis of PE.[1,2]Polymorphisms in SLC6A4 are associated with the incidence of lifelong premature ejaculation(LPR).The effects of 5-hydroxytryptamine(serotonin)transporter gene-linked polymorphic region(5-HTTLPR)and serotonin transporter gene intron 2(STin2)polymorphism on lifelong PE(LPE)are controversial.We aimed to determine possible relationships between 5-HTTLPR and STin2 polymorphisms in the SERT gene and clinical response of a selective serotonin reuptake inhibitor(dapoxetine)in LPE.
