Inhibitory effect of parvovirus H-1 on the formation of colonies of human hepatoma cell line in vitro and its tumors in nude mice

The inhibitory effect of parvovirus H-1 on the colonyforming ability in vitro of QGY-7703, a cultured human hepatoma cell line, and on the formation and growth of its tumors in nude mice was studied. With higher multiplicity of infection (MOI) of H-1 given, survival of the QGY-7703 cells was found to be decreased. H-1 DNA amplification level at 30 h postinfection(p.i.) was detected to be 7.4 times higher than that at 2 h by dispersed cells assay, while the cells were delayed to enter into S phase.Plaques were formed in the indicator cells (new-born human kidney cell line, NBK) by progeny H-1 virus particles released from the infected QGY-7703 cells by infectious cell center assay. The formation of tumors in nude mice by QGY-7703 cells which were injected s c at 2 h postinfection was observed to be prevented in 2 groups with given MOI 25 and 50. The tumor growth of MOI 10 group occurred at a lower exponential rate than that of control,after a 20 d latent period. It was evident that parvovirus H-1 exhibited a direct inhibitory effect on the formation and growth of human hepatoma cells in vivo as well as in vitro.

Temperature-mediated tribological characteristics of 40CrNiMoA steel and Inconel 718 alloy during sliding against Si_(3)N_(4) counterparts

A comparative evaluation of the friction and wear behaviors of 40CrNiMoA steel and Inconel 718 alloy sliding against Si_(3)N_(4) counterparts was conducted over a large temperature range from room temperature(RT)to 800℃.The temperature‐dependent tribological properties associated with the resulting chemical mitigation and structural adaptation of the solid sliding surface were clarified by surface/interface characterizations.The results revealed desirable performance in reducing friction and wear at elevated temperatures,which was associated with the resulting oxide composite filmʹs adaptive lubricating capability,whereas severe abrasive wear occurred at room/ambient temperatures.The oxidative‐abrasive differentials for the two alloys were further discussed by considering the combined effect of temperature and stressed‐shearing conditions.

Water-in-Water Emulsions,Ultralow Interfacial Tension,and Biolubrication

A water-in-water(W/W)emulsion consists of droplets formed by the spontaneous liquid-liquid separation of two immiscible aqueous phases.The inherent properties of the W/W interfaces,low or ultralowinterfacial tension(γ_(W/W)=1-1000μN/m)and a large thickness of several nanometers,beget the poor inherent stability of emulsions.Herein,we report a nanofibril emulsifier having Schiff base reactivity to generate a W/W emulsion.The W/W emulsion has superior stability(stable>6 months)because collagen nanofibrils,acting as a stabilizer of W/W emulsions,can simultaneously satisfy the requirements of sizeandoverall coverage ratio of the phase interfaces.W/Wemulsions having γ_(W/W)∼10μN/m were used as synthetic synovial fluids,showing superior lubrication performance with a coefficient of friction in the range of 0.003-0.011,which has been demonstrated to be suitable for joint lubrication.An intraarticular injection assessment further confirmed this protective effect on articular cartilage in vivo.Our study reveals the mechanism of emulsion stabilization and opens up the possibility of osteoarthritis(OA)treatment using the biolubrication effects of W/W emulsions for lubricated artificial implant surfaces.