As an emerging technology for pest monitoring, sound detection technology has the unique advantages in the field of plant quarantine. Experts at home and abroad have done lots of researches on sound detection technolo...As an emerging technology for pest monitoring, sound detection technology has the unique advantages in the field of plant quarantine. Experts at home and abroad have done lots of researches on sound detection technology for trunk borers, and achieved good results. The audible signals of adult Gnathotrichus mate- riarius and G. sulcatus were detected using sound detection method. It was concluded that the feeding and crawling acoustic signals of G. materiarius and G. sulca- tun were significantly different, and the acoustic signals were greatly different even in the same species. In addition, the signal pulse duration and dominant frequen- cy were significantly different. Therefore, it is feasible to monitor trunk borers using sound detection technology.展开更多
The paper aimed to establish a duplex PCR method for simultaneous detection of Pseudomonas savastanoi pv. Phaseolicola （Psp） and Curtobaeterium /accumfadens pv. Flaccumfaciens （Cff）. Based on the argK gene of Psp ...The paper aimed to establish a duplex PCR method for simultaneous detection of Pseudomonas savastanoi pv. Phaseolicola （Psp） and Curtobaeterium /accumfadens pv. Flaccumfaciens （Cff）. Based on the argK gene of Psp in GenBank, the primers PSPF1/PSPR2 were designed. The duplex PCR assay was dereloped using the combined primers PSPF1/PSPR2 and CflF1/CffR2, which were specific primers for Cff. The reaction conditions were optimized and specificity md sensitivity of the duplex PCR were tested. The expected DNA fragment was specifically amplified from the genomic DNA of Psp and Cff. Specificity was conirmed in the artificially inoculated soybean samples imparted. Thus, the duplex PCR developed in this study could be used for the simultaneous detection of Psp md Cff from imported soybean.展开更多
基金Supported by Research Project of Jiangsu Entry-Exit Inspection and Quarantine Bureau(2013KJ52)
文摘As an emerging technology for pest monitoring, sound detection technology has the unique advantages in the field of plant quarantine. Experts at home and abroad have done lots of researches on sound detection technology for trunk borers, and achieved good results. The audible signals of adult Gnathotrichus mate- riarius and G. sulcatus were detected using sound detection method. It was concluded that the feeding and crawling acoustic signals of G. materiarius and G. sulca- tun were significantly different, and the acoustic signals were greatly different even in the same species. In addition, the signal pulse duration and dominant frequen- cy were significantly different. Therefore, it is feasible to monitor trunk borers using sound detection technology.
基金Supported by Science and Technology Project of AQSIQ(2013IK277)National Rice Industry System Development Program
文摘The paper aimed to establish a duplex PCR method for simultaneous detection of Pseudomonas savastanoi pv. Phaseolicola （Psp） and Curtobaeterium /accumfadens pv. Flaccumfaciens （Cff）. Based on the argK gene of Psp in GenBank, the primers PSPF1/PSPR2 were designed. The duplex PCR assay was dereloped using the combined primers PSPF1/PSPR2 and CflF1/CffR2, which were specific primers for Cff. The reaction conditions were optimized and specificity md sensitivity of the duplex PCR were tested. The expected DNA fragment was specifically amplified from the genomic DNA of Psp and Cff. Specificity was conirmed in the artificially inoculated soybean samples imparted. Thus, the duplex PCR developed in this study could be used for the simultaneous detection of Psp md Cff from imported soybean.
