OBJECTIVE: To investigate the protective effect of L-arginine (L-arg) against liver ischemic-reperfusion (I/R) injury in rat model and its possible mechanism. METHODS: Male Sprague-Dawley rats were randomized into sha...OBJECTIVE: To investigate the protective effect of L-arginine (L-arg) against liver ischemic-reperfusion (I/R) injury in rat model and its possible mechanism. METHODS: Male Sprague-Dawley rats were randomized into sham group: hepatoduodenal ligament mobilized, but not clamped; control group: hepatic ischemia only; Arg group: 5 minutes before hepatic ischemia, L-arg (200 mg/kg) injected via the dorsal penis vein; Arg+L group: 10 and 5 minutes before hepatic ischemia, L-NAME (30 mg/kg) and L-arg (200 mg/kg) injected via the dorsal penis vein, respectively; and Fink group: 5 minutes before, hepatic ischemia, ZVAD-fmk (15 mg/kg) injected via the dorsal penis vein. The liver was subjected to ischemia for 40 minutes by Pringle's maneuver, and reperfusion was initiated hy removing clamp. The 7-day survival rate, alanine transaminase (ALT) level, caspase-3 activity and apoptotic hepatocyte count were compared among these groups. RESULTS: After 40 minutes of ischemia and 6 hours of reperfusion, the 7-day survival rate of the Arg group was significantly higher than that of the control and Arg+L groups (P<0.05). The ALT Level, caspase-3 activity and apoptotic hepatocyte count in the Arg group were decreased significantly than those in the control and Arg+L groups (P<0. 01). The caspase-3 activity and apoptotic hepatocyte count in the Arg group were slightly higher than those in the sham and Fmk groups, but there was no statistical significance. CONCLUSIONS: L-arg could ameliorate liver I/R injury and the possible protective mechanism is inhibition of hepatocyte apoptosis via inhibition of caspase-3 activity by nitric oxide synthesis.展开更多
BACKGROUND: Hilar cholangiocarcinoma (HCCA) is a devastating malignancy arising from the bifurcation of the hepatic duct, whether combined vascular resection benefits HCCA patients is controversial. This study was ...BACKGROUND: Hilar cholangiocarcinoma (HCCA) is a devastating malignancy arising from the bifurcation of the hepatic duct, whether combined vascular resection benefits HCCA patients is controversial. This study was undertaken to assess the effect of combined vascular resection in HCCA patients and to analyze the prognostic factors.展开更多
OBJECTIVE: To investigate whether hepatocyte apoptosis in hepatic ischemia-reperfusion (I/R) injury is mediated by Fas pathway. METHODS: Fas-mRNA expression detected by in situ hybridization assay, caspase-3 activity ...OBJECTIVE: To investigate whether hepatocyte apoptosis in hepatic ischemia-reperfusion (I/R) injury is mediated by Fas pathway. METHODS: Fas-mRNA expression detected by in situ hybridization assay, caspase-3 activity measured by fluorescence spectrophotometer, and hepatocyte apoptosis detected by TUNEL assay were compared in different L/R conditions between cirrhotic and normal rats. The relationship was analyzed between hepatocyte apoptosis, Fas-mRNA expression, and caspase-3 activity. RESULTS: In cirrhotic rats, Fas-mRNA expression and caspase-3 activity were significantly increased when the ischemic time prolonged, and subsequently, hepatocyte apoptosis was increased (P<0.O1). Under the same I/R condition, the Fas-mRNA expression, caspase-3 activity and hepatocyte apoptosis in cirrhotic liver were significantly higher than those in normal liver (P<0.O1). CONCLUSIONS: Hepatocyte apoptosis in hepatic I/R injury might be mediated by Fas pathway. The possible underlying mechanism that cirrhotic liver is more sensitive to ischemic injury than normal liver is alteration of Fas expression level.展开更多
The phase transition of titanium alloys is sensitive to the heat-treatment procedure,accompanied with the latent heat induced by phase transition.However,the latent heat during phase transition of titanium alloy has n...The phase transition of titanium alloys is sensitive to the heat-treatment procedure,accompanied with the latent heat induced by phase transition.However,the latent heat during phase transition of titanium alloy has not been systematically studied,which may result in the gap between designed and actual temperature of the sample and affect the final mechanical properties.In this work,DSC(differential scanning calorimetry)and first-principles simulate methods were used to study theβ→αphase transition process of TB18(Ti–Al-Mo-V-Cr-Nb-Fe system)metastableβtitanium alloy,especially to reveal the influence of the heating rate on latent heat.The ratio of latent heat to endothermic heat of the sample during temperature rising was introduced to interpret the effect of latent heat to actual temperature.The ratio of latent heat to endothermic heat at 1℃/min is about 15 to 20 times higher than that at 10℃/min.The higher ratio indicates that the latent heat of phase transition has a more significant effect on the temperature,which is related to the temperature range of phase transition and theαvolume fraction.Compared with the heating rate of 1℃/min,theβ→αphase transition takes place at higher temperature and the volume fraction ofαis smaller at 10℃/min.Meanwhile,there is a precipitation free zone between grain boundaryαand intragranularαand the distribution ofαlamellae is heterogeneous when the heating rate is 10℃/min.Both of the experimental and theoretical results suggest that the latent heat of phase transition is the main cause of the temperature fluctuation during heat-treatment process.This work has guiding significance for microstructure optimization affected by temperature,to achieve the desired mechanical properties.展开更多
文摘OBJECTIVE: To investigate the protective effect of L-arginine (L-arg) against liver ischemic-reperfusion (I/R) injury in rat model and its possible mechanism. METHODS: Male Sprague-Dawley rats were randomized into sham group: hepatoduodenal ligament mobilized, but not clamped; control group: hepatic ischemia only; Arg group: 5 minutes before hepatic ischemia, L-arg (200 mg/kg) injected via the dorsal penis vein; Arg+L group: 10 and 5 minutes before hepatic ischemia, L-NAME (30 mg/kg) and L-arg (200 mg/kg) injected via the dorsal penis vein, respectively; and Fink group: 5 minutes before, hepatic ischemia, ZVAD-fmk (15 mg/kg) injected via the dorsal penis vein. The liver was subjected to ischemia for 40 minutes by Pringle's maneuver, and reperfusion was initiated hy removing clamp. The 7-day survival rate, alanine transaminase (ALT) level, caspase-3 activity and apoptotic hepatocyte count were compared among these groups. RESULTS: After 40 minutes of ischemia and 6 hours of reperfusion, the 7-day survival rate of the Arg group was significantly higher than that of the control and Arg+L groups (P<0.05). The ALT Level, caspase-3 activity and apoptotic hepatocyte count in the Arg group were decreased significantly than those in the control and Arg+L groups (P<0. 01). The caspase-3 activity and apoptotic hepatocyte count in the Arg group were slightly higher than those in the sham and Fmk groups, but there was no statistical significance. CONCLUSIONS: L-arg could ameliorate liver I/R injury and the possible protective mechanism is inhibition of hepatocyte apoptosis via inhibition of caspase-3 activity by nitric oxide synthesis.
基金financial support from Pre-research Project of Equipment Development Department of China(No.41422010505)the Technology Innovation Leading Program of Shanxi Province,China(No.2019CGHJ-21)。
基金supported by a grant from the National Natural Science Foundation of China(81172039)
文摘BACKGROUND: Hilar cholangiocarcinoma (HCCA) is a devastating malignancy arising from the bifurcation of the hepatic duct, whether combined vascular resection benefits HCCA patients is controversial. This study was undertaken to assess the effect of combined vascular resection in HCCA patients and to analyze the prognostic factors.
文摘OBJECTIVE: To investigate whether hepatocyte apoptosis in hepatic ischemia-reperfusion (I/R) injury is mediated by Fas pathway. METHODS: Fas-mRNA expression detected by in situ hybridization assay, caspase-3 activity measured by fluorescence spectrophotometer, and hepatocyte apoptosis detected by TUNEL assay were compared in different L/R conditions between cirrhotic and normal rats. The relationship was analyzed between hepatocyte apoptosis, Fas-mRNA expression, and caspase-3 activity. RESULTS: In cirrhotic rats, Fas-mRNA expression and caspase-3 activity were significantly increased when the ischemic time prolonged, and subsequently, hepatocyte apoptosis was increased (P<0.O1). Under the same I/R condition, the Fas-mRNA expression, caspase-3 activity and hepatocyte apoptosis in cirrhotic liver were significantly higher than those in normal liver (P<0.O1). CONCLUSIONS: Hepatocyte apoptosis in hepatic I/R injury might be mediated by Fas pathway. The possible underlying mechanism that cirrhotic liver is more sensitive to ischemic injury than normal liver is alteration of Fas expression level.
基金the Strategic Priority Research Program of the Chinese Academy of Sciences(XDA22010101)the National Key Research and Development Program of China(2021YFC2801801)+1 种基金the CAS Project for Young Scientists in Basic Research(YSBR-025),the Natural Science Foundation of China(51871225)the Youth Innovation Promotion Association CAS and Liaoning Revitalization Talents Program(XLYC1907005).
文摘The phase transition of titanium alloys is sensitive to the heat-treatment procedure,accompanied with the latent heat induced by phase transition.However,the latent heat during phase transition of titanium alloy has not been systematically studied,which may result in the gap between designed and actual temperature of the sample and affect the final mechanical properties.In this work,DSC(differential scanning calorimetry)and first-principles simulate methods were used to study theβ→αphase transition process of TB18(Ti–Al-Mo-V-Cr-Nb-Fe system)metastableβtitanium alloy,especially to reveal the influence of the heating rate on latent heat.The ratio of latent heat to endothermic heat of the sample during temperature rising was introduced to interpret the effect of latent heat to actual temperature.The ratio of latent heat to endothermic heat at 1℃/min is about 15 to 20 times higher than that at 10℃/min.The higher ratio indicates that the latent heat of phase transition has a more significant effect on the temperature,which is related to the temperature range of phase transition and theαvolume fraction.Compared with the heating rate of 1℃/min,theβ→αphase transition takes place at higher temperature and the volume fraction ofαis smaller at 10℃/min.Meanwhile,there is a precipitation free zone between grain boundaryαand intragranularαand the distribution ofαlamellae is heterogeneous when the heating rate is 10℃/min.Both of the experimental and theoretical results suggest that the latent heat of phase transition is the main cause of the temperature fluctuation during heat-treatment process.This work has guiding significance for microstructure optimization affected by temperature,to achieve the desired mechanical properties.