Objective:Follistatin(FST)inhibits the action of activin by interfering with the binding of activin to its receptor.Although the prognostic value of FST in various cancers has been investigated previously,studies rare...Objective:Follistatin(FST)inhibits the action of activin by interfering with the binding of activin to its receptor.Although the prognostic value of FST in various cancers has been investigated previously,studies rarely focused on hypopharyngeal carcinoma(HPC).In our study,the effect of FST expression on HPC tissues and cell lines was investigated.Methods:A total of 60 patients with HPC were recruited for this study.Levels of FST mRNA and protein were measured by quantitative polymerase chain reaction(PCR)and immunohistochemistry in HPC tissue samples and by qPCR in the HPC FaDu cells,as well as immortal nasopharyngeal epithelial cell line NP-69 cells.After silencing the FST expression in FaDu cells using lentivirus-mediated siRNA that was specific for FST mRNA,cell proliferation was determined by a Celigo assay.Tumor growth was monitored in nude mice and viability was determined by a methylthiazoletetrazolium assay.The ratio of cell cycle arrest and apoptosis was evaluated by flow cytometry.The colony formation ability was performed using Giemsa staining.In addition,wound healing and Transwell migration and invasion assays were performed for the analysis of cell motility.Results:FST expression was significantly higher in human HPC tissue and FaDu cells than in normal tissue and NP-69 cells.A higher expression of FST in HPC samples was positively correlated with advanced tumors.Moreover,FST knockdown by shRNA significantly decreased cell growth,colony formation,migration and invasion.Furthermore,FST silencing increased the cell apoptosis percentage,and arrested cell cycle in the S phase in FaDu cells.In addition,FST silencing suppressed tumor growth in vivo.Conclusions:Our results indicated that the FST gene was associated with HPC progression and may serve as a potential therapeutic target for the treatment of HPC.展开更多
Sleep-disordered breathing （SDB） is the most common sleep- related disorder, and it can seriously affect sleep conditions, growth, and neurocognitive function. Recent research even demonstrates that hypertensive pat...Sleep-disordered breathing （SDB） is the most common sleep- related disorder, and it can seriously affect sleep conditions, growth, and neurocognitive function. Recent research even demonstrates that hypertensive patients with SDB are at an increased risk of developing resistant hypertension.展开更多
Two types of bifunctional bis（salicylaldimine） ligands（syn-L and anti-L） were designed and synthesized to support bimetallic aluminum complexes. Owing to the rigid anthracene skeleton, syn-L and anti-L successfull...Two types of bifunctional bis（salicylaldimine） ligands（syn-L and anti-L） were designed and synthesized to support bimetallic aluminum complexes. Owing to the rigid anthracene skeleton, syn-L and anti-L successfully locked two Al centers in close proximity（syn-Al2） and far apart（anti-Al2）, respectively. The distance between two Al centers in syn-Al2 was defined by X-ray diffraction as 6.665 ？, which is far shorter than that in anti-Al2. In the presence of stoichiometrical Bn OH, syn-Al2 and anti-Al2 were both efficient for ring-opening polymerization（ROP） of rac-LA with the former being more active. In the presence of excess Bn OH, syn-Al2 showed an efficient and immortal feature, consistent with high conversions, matched Mns, narrow molecular weight distributions and end group fidelity, while anti-Al2 had a much lower activity or even became entirely inactive due to rapid decomposition, indicated by in situ ～1H-NMR experiments of Al complexes with Bn OH.展开更多
基金supported by the Project of Young and Middle-aged Scientific Research Fund of Wannan Medical College(No.WK2019F11).
文摘Objective:Follistatin(FST)inhibits the action of activin by interfering with the binding of activin to its receptor.Although the prognostic value of FST in various cancers has been investigated previously,studies rarely focused on hypopharyngeal carcinoma(HPC).In our study,the effect of FST expression on HPC tissues and cell lines was investigated.Methods:A total of 60 patients with HPC were recruited for this study.Levels of FST mRNA and protein were measured by quantitative polymerase chain reaction(PCR)and immunohistochemistry in HPC tissue samples and by qPCR in the HPC FaDu cells,as well as immortal nasopharyngeal epithelial cell line NP-69 cells.After silencing the FST expression in FaDu cells using lentivirus-mediated siRNA that was specific for FST mRNA,cell proliferation was determined by a Celigo assay.Tumor growth was monitored in nude mice and viability was determined by a methylthiazoletetrazolium assay.The ratio of cell cycle arrest and apoptosis was evaluated by flow cytometry.The colony formation ability was performed using Giemsa staining.In addition,wound healing and Transwell migration and invasion assays were performed for the analysis of cell motility.Results:FST expression was significantly higher in human HPC tissue and FaDu cells than in normal tissue and NP-69 cells.A higher expression of FST in HPC samples was positively correlated with advanced tumors.Moreover,FST knockdown by shRNA significantly decreased cell growth,colony formation,migration and invasion.Furthermore,FST silencing increased the cell apoptosis percentage,and arrested cell cycle in the S phase in FaDu cells.In addition,FST silencing suppressed tumor growth in vivo.Conclusions:Our results indicated that the FST gene was associated with HPC progression and may serve as a potential therapeutic target for the treatment of HPC.
文摘Sleep-disordered breathing （SDB） is the most common sleep- related disorder, and it can seriously affect sleep conditions, growth, and neurocognitive function. Recent research even demonstrates that hypertensive patients with SDB are at an increased risk of developing resistant hypertension.
基金financially supported by the National Natural Science Foundation of China (No. B040102)State Key Laboratory for Modification of Chemical Fibers and Polymer Materials (Donghua University) (No. LK1501)Department of Science and Technology of Qingdao and Shandong Province (Nos. 159181jch and 2015GGX107015)
文摘Two types of bifunctional bis（salicylaldimine） ligands（syn-L and anti-L） were designed and synthesized to support bimetallic aluminum complexes. Owing to the rigid anthracene skeleton, syn-L and anti-L successfully locked two Al centers in close proximity（syn-Al2） and far apart（anti-Al2）, respectively. The distance between two Al centers in syn-Al2 was defined by X-ray diffraction as 6.665 ？, which is far shorter than that in anti-Al2. In the presence of stoichiometrical Bn OH, syn-Al2 and anti-Al2 were both efficient for ring-opening polymerization（ROP） of rac-LA with the former being more active. In the presence of excess Bn OH, syn-Al2 showed an efficient and immortal feature, consistent with high conversions, matched Mns, narrow molecular weight distributions and end group fidelity, while anti-Al2 had a much lower activity or even became entirely inactive due to rapid decomposition, indicated by in situ ～1H-NMR experiments of Al complexes with Bn OH.
