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沉默ADAM17对HT29人结肠癌细胞体外侵袭能力的影响及机制探究 被引量:2
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作者 张琪 杨光华 +5 位作者 黄志强 葛志鹏 刘少鹏 田炜 张国志 王长友 《中国现代医学杂志》 CAS 2019年第7期24-29,共6页
目的探究沉默解聚素-金属蛋白酶17(ADAM17)对HT29人结肠癌细胞体外侵袭能力的影响及机制。方法 HT29人结肠癌细胞经慢病毒转染处理后沉默ADAM17蛋白的表达,实时聚合酶链反应(real-time PCR)检测ADAM17 mRNA的表达,单层细胞划痕损伤修复... 目的探究沉默解聚素-金属蛋白酶17(ADAM17)对HT29人结肠癌细胞体外侵袭能力的影响及机制。方法 HT29人结肠癌细胞经慢病毒转染处理后沉默ADAM17蛋白的表达,实时聚合酶链反应(real-time PCR)检测ADAM17 mRNA的表达,单层细胞划痕损伤修复实验检测细胞迁移能力的变化,Transwell体外侵袭实验检测细胞侵袭能力的改变,Western blotting检测转染后MMP-9蛋白及ERK表达情况。结果经慢病毒转染处理后HT29细胞的ADAM17表达被抑制(P <0.05)。与空白对照组比较,沉默ADAM17的表达后,转染组细胞的迁移和侵袭能力被抑制(P <0.05);沉默ADAM17后,细胞基质金属蛋白酶9(MMP-9)表达水平下调、磷酸化细胞外调节蛋白激酶(p-ERK1/2)表达水平下降(P <0.05),但细胞外调节蛋白激酶(ERK)表达水平差异无统计学意义(P>0.05)。结论 ADAM17可能通过抑制ERK通路激活下调MMP-9的表达,从而影响细胞迁移、侵袭能力。 展开更多
关键词 结肠肿瘤 解聚素-金属蛋白酶17 转染 小干扰RNA 侵袭 细胞外调节蛋白激酶
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Professor Wei-bin GAO's experience in treatment of voiding dysfunction with electroacupuncture
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作者 shao-peng liu Ming-yuan HAN +5 位作者 Xin-yuan CAO Ying-ying ZHU Jian-tao YIN Zhong-ren SUN Wei-bin GAO Hong-na YIN 《World Journal of Acupuncture-Moxibustion》 CAS 2024年第4期325-331,共7页
As a common clinical syndrome,voiding dysfunction is complicated in etiology,involved in a variety of diseases and associated with multi-disciplines of medicine.Either medication or surgery has not obtained the favora... As a common clinical syndrome,voiding dysfunction is complicated in etiology,involved in a variety of diseases and associated with multi-disciplines of medicine.Either medication or surgery has not obtained the favorable effect on it.Integrated the theories of traditional Chinese medicine and Western medicine and based on the pathogenesis of the disease,the acupoint specificity and neuromodulatory effects,Professor Wei-bin GAO suggested"selecting the acupoints along the affected areas"The acupoints located near to the lumbar,sacral and abdominal regions are dominated and stimulated with electroacupuncture at different electric waves.In treatment,electric stimulation with disperse and dense waves was adopted.The bone conduction theory of dense-wave electric field was proposed.The same neuromodulation is presented in different diseases such as neurogenic bladder,pediatric enuresis,senile nocturia,benign prostatic hyerplasia,and postpartum of postoperative urination disorders.Hence,the same therapeutic method is adoptable to different diseases with the basic acupoint composition modified. 展开更多
关键词 Electroacupuncture Voiding dysfunction Wei-bin GAO Experience
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A NEW SMOOTHING APPROXIMATION METHOD FOR SOLVING BOX CONSTRAINED VARIATIONAL INEQUALITIES
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作者 Chang-feng Ma Guo-ping Liang shao-peng liu 《Journal of Computational Mathematics》 SCIE CSCD 2002年第5期533-542,共10页
In this paper, we first give a smoothing approximation function of nonsmooth system based on box constrained variational inequalities and then present a new smoothing approximation algorithm. Under suitable conditions... In this paper, we first give a smoothing approximation function of nonsmooth system based on box constrained variational inequalities and then present a new smoothing approximation algorithm. Under suitable conditions,we show that the method is globally and superlinearly convergent. A few numerical results are also reported in the paper. 展开更多
关键词 box constrained variational inequalities smoothing approximation global convergence superlinear convergence
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