As human stem cells with the special pluripotency play important roles in the innovative drug discovery and regenerative medicine,development of extracellular matrix(ECM)mimetics or functional materials that can suppo...As human stem cells with the special pluripotency play important roles in the innovative drug discovery and regenerative medicine,development of extracellular matrix(ECM)mimetics or functional materials that can support stem cell growth and propagation is of high significance.Despite numerous efforts spent,one major limitation restricting the wide applications of stem cells to the clinical translation is the lack of efficient strategies for low cost and large-scale stem cell production under xeno-free culture conditions.Herein,we reported a new strategy with peptides-modified polystyrene-based polymers coated onto the surface of coverslips for the growth and reproduction of human embryonic stem cells(h ESCs).The modified peptides are the active parts of proteins which has been shown to contribute to the pluripotent stem cell attachment or proliferation.The peptides were linked to the glass coverslips coated by the polymer materials via chemical crosslinking,and the composite substrates successfully maintain the longterm growth of HUES-7,H7 and DF699.Our study shows that the coating of polystyrene-derived polymer modified by our developed peptides is a good matrix for long-term growth and reproduction of stem cells.This polystyrene-derived polymer substrate can be produced in large scale and stored for a long time.The most important thing is that it can support the growth of undifferentiated human pluripotent stem cells(h PSCs)for more than ten passages,which could provide a new and relatively easy way to amplify h ESCs in vitro.展开更多
基金the National Natural Science Foundation of China(Nos.21877130 and U1801681)The Key Field Research and Development Program of Guangdong Province(No.2019B020235001)+2 种基金Basic and Applied Basic Research Foundation of Guangdong Province(No.2019A1515110451)Science and Technology Program of Guangzhou(No.201803010009)Natural Science Foundation of Guangdong Province(Nos.2018A030313600,2021A1515010357)。
文摘As human stem cells with the special pluripotency play important roles in the innovative drug discovery and regenerative medicine,development of extracellular matrix(ECM)mimetics or functional materials that can support stem cell growth and propagation is of high significance.Despite numerous efforts spent,one major limitation restricting the wide applications of stem cells to the clinical translation is the lack of efficient strategies for low cost and large-scale stem cell production under xeno-free culture conditions.Herein,we reported a new strategy with peptides-modified polystyrene-based polymers coated onto the surface of coverslips for the growth and reproduction of human embryonic stem cells(h ESCs).The modified peptides are the active parts of proteins which has been shown to contribute to the pluripotent stem cell attachment or proliferation.The peptides were linked to the glass coverslips coated by the polymer materials via chemical crosslinking,and the composite substrates successfully maintain the longterm growth of HUES-7,H7 and DF699.Our study shows that the coating of polystyrene-derived polymer modified by our developed peptides is a good matrix for long-term growth and reproduction of stem cells.This polystyrene-derived polymer substrate can be produced in large scale and stored for a long time.The most important thing is that it can support the growth of undifferentiated human pluripotent stem cells(h PSCs)for more than ten passages,which could provide a new and relatively easy way to amplify h ESCs in vitro.
