滚环扩增技术鉴定念珠菌、曲霉和赛多孢子菌

由于机会感染真菌对抗真菌药物的敏感性存在很大的差异，因此，及时鉴定机会感染真菌到种的水平对于后续临床治疗具有重要意义。为了克服传统培养鉴定方法耗时和敏感性差的缺陷，针对真菌核糖体DNA基因复合体ITS区，在PCR技术基础上建立了许多分子生物学鉴定技术，如多重和（或）实时PCR、DNA序列分析和探针相关技术。滚环扩增技术能检测到数个碱基甚至单个碱基的差异，显示出很好的敏感性和特异性。我们采用滚环扩增技术，鉴定机会感染真菌包括除白念珠菌和烟曲霉以外的念珠菌和曲霉，以及赛多孢子菌。

Proposed nomenclature for Pseudallescheria,Scedosporium and related genera

As a result of fundamental changes in the International Code of Nomenclature on the use of separate names for sexual and asexual stages of fungi,generic names of many groups should be reconsidered.Members of the ECMM/ISHAM working group on Pseudallescheria/Scedosporium infections herein advocate a novel nomenclature for genera and species in Pseudallescheria,Scedosporium and allied taxa.The generic names Parascedosporium,Lomentospora,Petriella,Petriellopsis,and Scedosporium are proposed for a lineage within Microascaceae with mostly Scedosporium anamorphs producing slimy,annellidic conidia.Considering that Scedosporium has priority over Pseudallescheria and that Scedosporium prolificans is phylogenetically distinct from the other Scedosporium species,some name changes are proposed.Pseudallescheria minutispora and Petriellidium desertorum are renamed as Scedosporium minutisporum and S.desertorum,respectively.Scedosporium prolificans is renamed as Lomentospora prolificans.

解封后的武汉生活已不同以往

在居家隔离两个多月之后,随着新增感染病例从2月中旬的每日几千例降至每周仅数例,1100万武汉居民当中的大多数人现在已经可以自由外出了。但是,对餐馆老板熊飞(Xiong Fei)来说,这座最早暴发新冠疫情的中国城市的解封并没有让他感到轻松,只是带来了一系列新的挑战。