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Distribution Characteristics of Pb and Its Ecological Risk in Different Farmland Soils of Changchun Suburb
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作者 Su Wei Liang Dongmei +1 位作者 Chen Minghui shen guisheng 《Meteorological and Environmental Research》 CAS 2014年第4期42-45,共4页
Pb in the farmland surface soil of Changchun suburb was taken as the main research object. The field research and laboratory analysis were used to investigate Pb content, spatial distribution characteristics and its e... Pb in the farmland surface soil of Changchun suburb was taken as the main research object. The field research and laboratory analysis were used to investigate Pb content, spatial distribution characteristics and its ecological risk in different farmland soils. The results showed that Pb content was during 18.85 - 41.34 mg/kg in farmland soil of Changchun suburb, and was higher than the background value of Jilin Province at 90% of the sampling sites. The spatial distribution of Pb content in farmland soil had obvious geographical features, and Pb content in southwest and northeast soils was higher. The high-content areas were relatively concentrated, and Pb content gradually reduced from the high-content island- shape center to its surrounding. The soil Pb contents in paddy field, dry field and vegetable field had insignificant difference with the background value, indicating that Pb accumulations in dry field, paddy field and vegetable field were not significant. The order of Pb content in different land use types was showed as follows: vegetable field 〉 dry field 〉 paddy field. Pb pollution of all samples belonged to light pollution. The average potential ecological risk of soil Pb in different land use types presented the following order: vegetable field 〉 dry field 〉 paddy field. 展开更多
关键词 Changchun suburb Farmland soil Heavy metal Pb Distribution characteristic Ecological risk China
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miR-214-5p靶向SIRT2介导白细胞介素-1β诱导软骨细胞损伤的机制研究
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作者 赵玉兰 沈桂生 +2 位作者 袁本前 陈洁 黄遵任 《当代医学》 2020年第22期1-6,共6页
目的探讨微小RNA-214-5p(miR-214-5p)对白细胞介素-1β(IL-1β)诱导的软骨细胞损伤的影响及其分子机制。方法分离培养SD大鼠关节软骨细胞,用IL-1β处理细胞,采用qRT-PCR与Western blot检测miR-214-5p与SIRT2表达。软骨细胞分别转染anti-... 目的探讨微小RNA-214-5p(miR-214-5p)对白细胞介素-1β(IL-1β)诱导的软骨细胞损伤的影响及其分子机制。方法分离培养SD大鼠关节软骨细胞,用IL-1β处理细胞,采用qRT-PCR与Western blot检测miR-214-5p与SIRT2表达。软骨细胞分别转染anti-miR-214-5p、pcDNA-SIRT2,检测IL-1β诱导后细胞中miR-214-5p与SIRT2表达,采用流式细胞术检测细胞凋亡。利用生物信息学预测miR-214-5p的靶基因,应用双荧光素酶报告实验及Western blot方法对靶基因进行验证。将si-SIRT2转染软骨细胞,检测IL-1β诱导后软骨细胞凋亡率。采用酶联免疫吸附法(ELISA)检测细胞中IL-6、TNF-α、IFN-γ的含量;采用Western blot检测细胞中Bax、Bcl-2表达。结果IL-1β处理软骨细胞后,细胞中miR-214-5p表达水平升高,SIRT2表达水平降低;IL-1β处理后的软骨细胞凋亡增多,IL-6、TNF-α、IFN-γ含量与Bax蛋白水平升高,Bcl-2蛋白水平降低;抑制miR-214-5p表达或SIRT2过表达的软骨细胞经IL-1β诱导后,细胞凋亡率降低,IL-6、TNF-α、IFN-γ含量与Bax蛋白水平降低,Bcl-2蛋白水平升高;miR-214-5p过表达可抑制含miR-214-5p结合位点荧光报告载体的强度,将结合位点突变后抑制作用消失;抑制SIRT2表达可逆转抑制miR-214-5p表达对IL-1β诱导软骨细胞损伤的保护作用。结论抑制miR-214-5p表达可靶向SIRT2对IL-1β诱导的软骨细胞损伤发挥保护作用,其作用机制与减少软骨细胞凋亡及抑制炎性反应有关。 展开更多
关键词 miR-214-5p SIRT2 IL-1Β 软骨细胞 凋亡
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