Diabetic peripheral neuropathy(DPN)is a chronic microvascular complication of diabetes.However,studies on effect of acupuncture in DPN are rare.The purpose of this study was to research the mechanism of acupuncture in...Diabetic peripheral neuropathy(DPN)is a chronic microvascular complication of diabetes.However,studies on effect of acupuncture in DPN are rare.The purpose of this study was to research the mechanism of acupuncture in DPN rats.Rats rendered diabetic with a single injection of 35 mg/kg STZ.The STZ-diabetic rats were randomly divided into two groups:DPN model and acupuncture groups.After 14 days treatment,the therapeutic efficacy of acupuncture was assessed using MWT and TWI evaluation of rats.展开更多
OBJECTIVE:To investigate the effect and mechanisms of Danzhi Jiangtang capsule(丹蛭降糖胶囊,DJC)on renal injury in streptozotocin(STZ)-induced diabetes of rats.METHODS:Sprague-Dawley rats were fed with high fat diet f...OBJECTIVE:To investigate the effect and mechanisms of Danzhi Jiangtang capsule(丹蛭降糖胶囊,DJC)on renal injury in streptozotocin(STZ)-induced diabetes of rats.METHODS:Sprague-Dawley rats were fed with high fat diet for 6 weeks followed by streptozotocin(STZ,35 mg/kg)injection.These rats were then treated with DJC(270,540 and 1080 mg/kg)daily for 8 weeks.RESULTS:A combination of high fat diet and STZ significantly increased blood glucose creatinine,urea nitrogen,and urine albumin in rats.Meanwhile,the glomerular and tubular lesions were observed in rats fed with high fat diet and injected with STZ.These biochemical and pathological changes were significantly attenuated by DJC treatments in a dose-dependent manner.Mechanistically,DJC treatments significantly decreased toll-like receptor 4(TLR4),mitogen-activated protein kinase(MAPK),and nuclear factor-κB(NF-κB)signals in the kidney of rats fed with high fat diet and injected with STZ.Terminal deoxynucleotidyl transferase dU TP nick end labeling staining and caspase-8 levels showed that renal apoptosis was increased in rats fed with high fat diet and injected with STZ,and this was attenuated by DJC treatments.CONCLUSIONS:DJC treatments protect against diabetic kidney disease,and the mechanism may be closely related to downregulation of TLR4/MAPK/NF-κB pathways and apoptosis.This study provides further evidence of using DJC as a potential therapeutic option for diabetic kidney disease.展开更多
文摘Diabetic peripheral neuropathy(DPN)is a chronic microvascular complication of diabetes.However,studies on effect of acupuncture in DPN are rare.The purpose of this study was to research the mechanism of acupuncture in DPN rats.Rats rendered diabetic with a single injection of 35 mg/kg STZ.The STZ-diabetic rats were randomly divided into two groups:DPN model and acupuncture groups.After 14 days treatment,the therapeutic efficacy of acupuncture was assessed using MWT and TWI evaluation of rats.
基金Supported by the Research Grant from the National Natural Science Foundation of China:Mechanism of Danzhi Jiangtang Capsule Regulating Adipocyte-derived Exosomes in the Prevention and Treatment of Type 2 Diabetes Mellitus Macrovascular Disease(No.82174153)Based on Chip Technology to Carry out Basic and Clinical Comparison to Explore the Research of Danzhi Jiangtang Capsule in Precise Treatment of Diabetic Macrovascular Disease(No.81774286)。
文摘OBJECTIVE:To investigate the effect and mechanisms of Danzhi Jiangtang capsule(丹蛭降糖胶囊,DJC)on renal injury in streptozotocin(STZ)-induced diabetes of rats.METHODS:Sprague-Dawley rats were fed with high fat diet for 6 weeks followed by streptozotocin(STZ,35 mg/kg)injection.These rats were then treated with DJC(270,540 and 1080 mg/kg)daily for 8 weeks.RESULTS:A combination of high fat diet and STZ significantly increased blood glucose creatinine,urea nitrogen,and urine albumin in rats.Meanwhile,the glomerular and tubular lesions were observed in rats fed with high fat diet and injected with STZ.These biochemical and pathological changes were significantly attenuated by DJC treatments in a dose-dependent manner.Mechanistically,DJC treatments significantly decreased toll-like receptor 4(TLR4),mitogen-activated protein kinase(MAPK),and nuclear factor-κB(NF-κB)signals in the kidney of rats fed with high fat diet and injected with STZ.Terminal deoxynucleotidyl transferase dU TP nick end labeling staining and caspase-8 levels showed that renal apoptosis was increased in rats fed with high fat diet and injected with STZ,and this was attenuated by DJC treatments.CONCLUSIONS:DJC treatments protect against diabetic kidney disease,and the mechanism may be closely related to downregulation of TLR4/MAPK/NF-κB pathways and apoptosis.This study provides further evidence of using DJC as a potential therapeutic option for diabetic kidney disease.
