Bacillus subtilis HAS had good control effects on sugarcane smut in the field. Colonization dynamics of B. subtilis HAS were studied through biotics marker and pot culture, to understand the bio-control mechanism o...Bacillus subtilis HAS had good control effects on sugarcane smut in the field. Colonization dynamics of B. subtilis HAS were studied through biotics marker and pot culture, to understand the bio-control mechanism of the strain and provide experimental basis for commercialization. The results showed that B.subtilis HAS had strong colonization ability, which could be colonized in sugarcane root, stem, leaf and rhizosphere for long time, but the colonization levels were different. The colonization density of B.sutili HAS remained 103 CFU/g soil in the rhizosphere and 102 CFU/g （fresh weight） in the root after inoculation of 60 d. The colonization in the rhizosphere decreased first, then increased and decreased again, and finally stabilized. The colonization in the root first increased then decreased. Meantime, colonization status in stems and leaves showed that the colonization density in stems and leaves was 10 CFU/g （fresh weight）, indicating that the colonization level was not high in stem and leaf.展开更多
To understand virus infection situation of integrated demonstration and regional test sugarcane varieties in national sugarcane system in Guilin City,Guangxi Province,leaf samples with or without symptoms were collect...To understand virus infection situation of integrated demonstration and regional test sugarcane varieties in national sugarcane system in Guilin City,Guangxi Province,leaf samples with or without symptoms were collected from 37 sugarcane varieties,and five kinds of viruses were detected by RT-PCR with specific primers,including Sugarcane yellow leaf virus( SCYLV),Sugarcane streak mosaic virus( SCSMV),Sorghum mosaic virus( SrM V),Sugarcane mosaic virus( SCMV) and Sugarcane bacilliform virus( SCBV). The results showed that SCYLV was detected from four sugarcane varieties,and the detection rate was10. 81%; SCSMV was detected from five sugarcane varieties,and the detection rate was 13. 51%; SrM V was detected from four sugarcane varieties,and the detection rate was 10. 81%; SCMV had not been found; SCBV was detected from 27 sugarcane varieties,and the detection rate was 72. 9%. Additionally,mixed infection of different viruses was widespread,and the total mixed infection rate was 21. 62%. Thus,integrated demonstration and regional test sugarcane varieties in national sugarcane system in Guilin City had been seriously infected by viruses,and mixed infection of different viruses was common. The paper will provide a reference for breeding and popularization of antiviral sugarcane varieties.展开更多
[Objective]The paper was to clarify the occurrence of sugarcane ratoon stunting disease(RSD)in Hainan sugarcane-growing areas.[Method]In 2019,270 samples of sugarcane leaves were collected from six main sugarcane-grow...[Objective]The paper was to clarify the occurrence of sugarcane ratoon stunting disease(RSD)in Hainan sugarcane-growing areas.[Method]In 2019,270 samples of sugarcane leaves were collected from six main sugarcane-growing areas in Hainan Province,and RSD was detected by PCR assay with specific primers.[Result]RSD was detected out in 41 out of 270 sugarcane samples,with an average detection rate of15.19%.The detection rates of RSD were different in six sugarcane-growing areas;the detection rate of RSD in Danzhou sugarcane-growing area was the highest of 22.00%;the detection rate of RSD in Lingao sugarcane-growing area was the lowest of 9.26%.RSD was detected out in 8 out of10 main sugarcane cultivars,among which Xintaitang 22 suffered the heaviest damage,with the positive detection rate of 45.83%;RSD had not been detected out in Zhongtang 1 and Zhongtang 2,while the positive detection rates of RSD in the remaining seven sugarcane cultivars were10.00%-31.25%.[Conclusion]RSD commonly occurs in Hainan sugarcane-growing areas.The research results provide a basis for scientific prevention and control of RSD and promotion and application of healthy virus-free sugarcane seedlings.展开更多
[Objective] The paper was to identify strain HN-1 against banana wilt disease and to determine its antagonism. [Method] The strain HN-1 was ob- tained from the soil in fields heavily infected by Fusarium oxysporum f. ...[Objective] The paper was to identify strain HN-1 against banana wilt disease and to determine its antagonism. [Method] The strain HN-1 was ob- tained from the soil in fields heavily infected by Fusarium oxysporum f. sp. cubense （FOC）. Antagonism of the strain against F. oxysporum was tested via dual-cul- ture and inhibition test on spore germination. [Result] HN-1 effectively inhibited mycelial growth and spore germination of F. oxysporum, Strain HN-1 was identi- fied as BrevibaciUus brevis according to its characteristics in morphology, physiology and biochemistry and its 16S rDNA sequence. The strain showed high inhibition effect on 15 species of fungal pathogens in the dual-culture trials with fungal pathogens. [ Conclusion] The study provides theoretical basis for application of strain HN-1 in agricultural fields.展开更多
采用文献计量和知识图谱方法分析近10年火针临床随机对照试验(RCT)文献的研究热点、前沿与趋势。检索中国期刊全文数据库(CNKI)、万方数据知识服务平台(Wanfang)、维普资讯中文期刊服务平台(VIP)、中国生物医学文献数据库(SinoMed)、Pub...采用文献计量和知识图谱方法分析近10年火针临床随机对照试验(RCT)文献的研究热点、前沿与趋势。检索中国期刊全文数据库(CNKI)、万方数据知识服务平台(Wanfang)、维普资讯中文期刊服务平台(VIP)、中国生物医学文献数据库(SinoMed)、PubMed、Web of Science(WOS)共6个中英文数据库中有关火针的RCT研究文献,采用CiteSpaceⅤ6.1.R6及VOSviewerⅤ1.6.18软件进行合作网络、关键词共现、关键词聚类、关键词时间线、关键词突现等分析,并绘制可视化知识图谱。共纳入1 973篇中文文献、3篇英文文献;发文量前3位的机构分别为广州中医药大学、黑龙江中医药大学及首都医科大学附属北京中医医院;火针多与针刺、拔罐及放血疗法联合应用治疗痤疮、白癜风、腰椎间盘突出症、带状疱疹、中风后遗症、面神经麻痹、膝关节骨关节炎等;研究前沿包括火针与其他疗法的联合应用、临床机制研究及疗效评价指标研究。未来应拓展优势病种,优化研究设计,加强团队之间的合作,深入开展高水平的临床研究。展开更多
基金Supported by National Natural Science Foundation of China(31471555)Basic Scientific Research Fund of Central Public Welfare Research Institutes(1630052016010)China Agricultural Industry Research System(CARS-170301)
文摘Bacillus subtilis HAS had good control effects on sugarcane smut in the field. Colonization dynamics of B. subtilis HAS were studied through biotics marker and pot culture, to understand the bio-control mechanism of the strain and provide experimental basis for commercialization. The results showed that B.subtilis HAS had strong colonization ability, which could be colonized in sugarcane root, stem, leaf and rhizosphere for long time, but the colonization levels were different. The colonization density of B.sutili HAS remained 103 CFU/g soil in the rhizosphere and 102 CFU/g （fresh weight） in the root after inoculation of 60 d. The colonization in the rhizosphere decreased first, then increased and decreased again, and finally stabilized. The colonization in the root first increased then decreased. Meantime, colonization status in stems and leaves showed that the colonization density in stems and leaves was 10 CFU/g （fresh weight）, indicating that the colonization level was not high in stem and leaf.
基金Supported by Special Fund of China Agricultural(Sugar)Industry Research System(CARS-170301)Basal Research Fund of Central Public Welfare Research Institutes(1630052016010)
文摘To understand virus infection situation of integrated demonstration and regional test sugarcane varieties in national sugarcane system in Guilin City,Guangxi Province,leaf samples with or without symptoms were collected from 37 sugarcane varieties,and five kinds of viruses were detected by RT-PCR with specific primers,including Sugarcane yellow leaf virus( SCYLV),Sugarcane streak mosaic virus( SCSMV),Sorghum mosaic virus( SrM V),Sugarcane mosaic virus( SCMV) and Sugarcane bacilliform virus( SCBV). The results showed that SCYLV was detected from four sugarcane varieties,and the detection rate was10. 81%; SCSMV was detected from five sugarcane varieties,and the detection rate was 13. 51%; SrM V was detected from four sugarcane varieties,and the detection rate was 10. 81%; SCMV had not been found; SCBV was detected from 27 sugarcane varieties,and the detection rate was 72. 9%. Additionally,mixed infection of different viruses was widespread,and the total mixed infection rate was 21. 62%. Thus,integrated demonstration and regional test sugarcane varieties in national sugarcane system in Guilin City had been seriously infected by viruses,and mixed infection of different viruses was common. The paper will provide a reference for breeding and popularization of antiviral sugarcane varieties.
基金Supported by National Natural Science Foundation of China(31771865)National Sugar Industry Technology System"Scientists for Prevention and Control of Sugarcane Virus Diseases"(CARS-170301)。
文摘[Objective]The paper was to clarify the occurrence of sugarcane ratoon stunting disease(RSD)in Hainan sugarcane-growing areas.[Method]In 2019,270 samples of sugarcane leaves were collected from six main sugarcane-growing areas in Hainan Province,and RSD was detected by PCR assay with specific primers.[Result]RSD was detected out in 41 out of 270 sugarcane samples,with an average detection rate of15.19%.The detection rates of RSD were different in six sugarcane-growing areas;the detection rate of RSD in Danzhou sugarcane-growing area was the highest of 22.00%;the detection rate of RSD in Lingao sugarcane-growing area was the lowest of 9.26%.RSD was detected out in 8 out of10 main sugarcane cultivars,among which Xintaitang 22 suffered the heaviest damage,with the positive detection rate of 45.83%;RSD had not been detected out in Zhongtang 1 and Zhongtang 2,while the positive detection rates of RSD in the remaining seven sugarcane cultivars were10.00%-31.25%.[Conclusion]RSD commonly occurs in Hainan sugarcane-growing areas.The research results provide a basis for scientific prevention and control of RSD and promotion and application of healthy virus-free sugarcane seedlings.
基金Supported by Special Funds for Construction of Modern Agricultural Technology System(nycytx-24)Fundamental Research Special Project for Central Public-interest Scientific Institutes(ITBB130502)
文摘[Objective] The paper was to identify strain HN-1 against banana wilt disease and to determine its antagonism. [Method] The strain HN-1 was ob- tained from the soil in fields heavily infected by Fusarium oxysporum f. sp. cubense （FOC）. Antagonism of the strain against F. oxysporum was tested via dual-cul- ture and inhibition test on spore germination. [Result] HN-1 effectively inhibited mycelial growth and spore germination of F. oxysporum, Strain HN-1 was identi- fied as BrevibaciUus brevis according to its characteristics in morphology, physiology and biochemistry and its 16S rDNA sequence. The strain showed high inhibition effect on 15 species of fungal pathogens in the dual-culture trials with fungal pathogens. [ Conclusion] The study provides theoretical basis for application of strain HN-1 in agricultural fields.
文摘采用文献计量和知识图谱方法分析近10年火针临床随机对照试验(RCT)文献的研究热点、前沿与趋势。检索中国期刊全文数据库(CNKI)、万方数据知识服务平台(Wanfang)、维普资讯中文期刊服务平台(VIP)、中国生物医学文献数据库(SinoMed)、PubMed、Web of Science(WOS)共6个中英文数据库中有关火针的RCT研究文献,采用CiteSpaceⅤ6.1.R6及VOSviewerⅤ1.6.18软件进行合作网络、关键词共现、关键词聚类、关键词时间线、关键词突现等分析,并绘制可视化知识图谱。共纳入1 973篇中文文献、3篇英文文献;发文量前3位的机构分别为广州中医药大学、黑龙江中医药大学及首都医科大学附属北京中医医院;火针多与针刺、拔罐及放血疗法联合应用治疗痤疮、白癜风、腰椎间盘突出症、带状疱疹、中风后遗症、面神经麻痹、膝关节骨关节炎等;研究前沿包括火针与其他疗法的联合应用、临床机制研究及疗效评价指标研究。未来应拓展优势病种,优化研究设计,加强团队之间的合作,深入开展高水平的临床研究。
