Furniture of Hongmu,cream of Chinese culture,has a long history and enjoys great popularity for its classic appearance and elegant disposition. The traditional method of the identification is to evaluate the macro for...Furniture of Hongmu,cream of Chinese culture,has a long history and enjoys great popularity for its classic appearance and elegant disposition. The traditional method of the identification is to evaluate the macro form and anatomical features.In this paper,a new method based on the differences among the chemical characteristics of biological metabolites of samples is developed.In the research, high performance liquid chromatography(HPLC) fingerprints were employed to discriminate four species of Hongmu,including Pterocarpus indicus, Pterocarpus macarocarpus,Dalbergia melanoxylon, and Dalbergia louvelii.The results showed that, according to the retention time and relative peak area of different components in wood extracts,the distinction can be easily made between different species.Based on the HPLC fingerprints,similarities between samples were evaluated by correlation coefficients.The results were greater than 0.81 between the same species,and less than 0.21 between different species,which showed weak correlation between different species.In order to estimate the overall features of the fingerprints, hierarchical clustering and principal component analysis(PCA) were used and the results showed pronounced clustering effect among the same species.The investigation indicated that pattern recognition could accurately reflect the significant differences between different species of Hongmu, and can be used as a reference for quality control.展开更多
文摘本实验旨在分析绿豆皮多糖(mung bean skin polysaccharide,MBSP)的结构表征和抗氧化活性.结果表明:MBSP是一种相对分子量362.66kD、单糖组成及摩尔比为阿拉伯糖:半乳糖:半乳糖醛酸:木糖:葡萄糖=28.45∶4.67∶3.29∶1.51∶1.00的酸性多糖;红外光谱结果显示存在多糖特征吸收峰,扫描电镜等结果显示其表面光滑且呈片状分布,是一种热稳定性较弱的半晶型聚合物;平均粒径(107.0±3.4)nm、Zeta电位(—11.6±0.11)mV;流变学分析结果显示MBSP溶液体系倾向于形成弱凝胶结构.体外抗氧化活性研究结果表明,MBSP具有良好的1,1G二苯基G2G三硝基苯肼(doctoral programs in publichealth,DPPH)自由基和2,2G连氮基—双G(3G乙基苯并二氢噻唑啉G6G磺酸)(agreement on basic telecommunicationservices,ABTS)自由基清除能力.此外,H2O2诱导的大鼠肠上皮细胞(IECG6细胞)的氧化损伤研究结果表明:MBSP能够显著提高氧化损伤细胞的存活率,有效提高超氧化物歧化酶(superoxidedismutase,SOD)的活力和降低丙二醛(malondialdehyde,MDA)含量,具有明显的氧化损伤保护作用,为MBSP的开发和利用提供了一定的理论基础.
文摘Furniture of Hongmu,cream of Chinese culture,has a long history and enjoys great popularity for its classic appearance and elegant disposition. The traditional method of the identification is to evaluate the macro form and anatomical features.In this paper,a new method based on the differences among the chemical characteristics of biological metabolites of samples is developed.In the research, high performance liquid chromatography(HPLC) fingerprints were employed to discriminate four species of Hongmu,including Pterocarpus indicus, Pterocarpus macarocarpus,Dalbergia melanoxylon, and Dalbergia louvelii.The results showed that, according to the retention time and relative peak area of different components in wood extracts,the distinction can be easily made between different species.Based on the HPLC fingerprints,similarities between samples were evaluated by correlation coefficients.The results were greater than 0.81 between the same species,and less than 0.21 between different species,which showed weak correlation between different species.In order to estimate the overall features of the fingerprints, hierarchical clustering and principal component analysis(PCA) were used and the results showed pronounced clustering effect among the same species.The investigation indicated that pattern recognition could accurately reflect the significant differences between different species of Hongmu, and can be used as a reference for quality control.
