Universal peroxidase-like strategy for sensitive glucose detection in complex matrix

Glucose detection in complex matrix such as physiological fluids and drinks can provide useful information guide for people.However,traditional detection methods toward complex matrix suffer from the impurity interference or complex pretreatments.So,it is important to exploit a universal and sensitive glucose detection strategy in complex matrix.In this work,a cascade catalytic scheme based on peroxidase-like MBs@MIL-100(Fe)@Ag(MMA)is developed for sensitive glucose detection in complex solution.Using 3,3’,5,5’-tetramethylbenzidine(TMB)as an indicator,MMA can trigger catalytic cascade reactions for specific glucose sensing.In particular,the peroxidase-like MIL-100(Fe)serves as both the catalysis unit and enrichment unit.Oxidation state of TMB(oxTMB)can be effectively and specifically enriched by MIL-100(Fe)to exclude the interference of undesired impurities and macromolecules,which is suitable for complex sample matrix including colored soda and saliva.In addition,utilizing the peroxidase-like activity of MIL-100(Fe)for self-clean,the residual indicator molecules can be degraded,resulting in the recyclable use of MMA.

In situ probing of cell-cell communications with surface- enhanced Raman scattering （SERS） nanoprobes and microfluidic networks for screening of immunotherapeutic drugs

Discovering novel drugs for cancer immunotherapy requires a robust in vitro drug screening platform that allows for straightforward probing of cell-ceil communications. Here, we combined surface-enhanced Raman scattering （SERS） nanoprobes with microfluidic networks to monitor in situ the cancer-immune system intercellular communications. The microfluidic platform links up immune cells with cancer cells, where the cancer-cell secretions act as signaling mediators. First, gold@silver core--shell nanorods were employed to fabricate SERS immunoprobes for analysis of the signaling molecules. Multiple cancer secretions in a tumor microenvironment were quantitatively analyzed by a SERS-assisted three-dimensional （3D） barcode immunoassay with high sensitivity （1 ng/mL）. Second, in an on-chip cell proliferation assay, multiple immunosuppressive proteins secreted by cancer cells were found to inhibit activation of immune cells, indicating that the platform simulates the physiological process of cancer-immune system communications. Furthermore, potential drug candidates were tested on this platform. A quantitative SERS immunoassay was performed to evaluate drug efficacy at regulating the secretion behavior of cancer cells and the activity of immune cells. This assay showed the suitability of this platform for in vitro drug screening. It is expected that the fully integrated and highly automated SERS-microfluidic platform will become a powerful analytical tool for probing intercellular communications and should accelerate the discovery and clinical validation of novel druKs.

2D profiling of tumor chemotactic and molecular phenotype at single cell resolution using a SERS-microfluidic chip

Emerging single-cell technologies create new opportunities for unraveling tumor heterogeneity.However,the development of high-content phenotyping platform is still at its infancy.Here,we develop a microfluidic chip for two-dimensional(2D)profiling of tumor chemotactic and molecular features at single cell resolution.Individual cells were captured by the triangular micropillar arrays in the cell-loading channel,facilitating downstream single-cell analysis.For 2D phenotyping,the chemotactic properties of tumor cells were visualized through cellular migratory behavior in microchannels,while their protein expression was profiled with multiplex surface enhanced Raman scattering(SERS)nanovectors,in which Raman reporter-embedded gold@silver core-shell nanoparticles(Au@Ag REPs)were modified with DNA aptamers targeting cellular surface proteins.As a proof of concept,breast cancer cells with diverse phenotypes were tested on the chip,demonstrating the capability of this platform for simultaneous chemotactic and molecular analysis.The chip is expected to provide a powerful tool for investigating tumor heterogeneity and promoting clinical precision medicine.