Diabetes,a prevalent chronic metabolic disorder,often leads to severe complications.Currently,existing treatment methods may pose life-threatening risks due to poor patient compliance and inaccurate dosing of subcutan...Diabetes,a prevalent chronic metabolic disorder,often leads to severe complications.Currently,existing treatment methods may pose life-threatening risks due to poor patient compliance and inaccurate dosing of subcutaneous insulin injections.Hence,a biomimetic red blood cell(RBC)membrane-coated glucose-responsive nanoplatform is developed for controlling insulin release.Functionalizing nanoplatforms with RBC membrane can prolong the half-life of nano-formulation in vivo mediated by the biomimetic immune escape.Simultaneously,the cascade catalytic effect of glucose oxidase(GOx)encapsulated in metal-organic frameworks(MOFs)and hemoglobin(Hb)in the RBC membrane are able to not only facilitate glucose-responsive insulin release,but also eliminate the detrimental by-product hydrogen peroxide(H_(2)O_(2))resulting from the Hb mediated H_(2)O_(2)scavenging.Both in vitro and in vivo studies have demonstrated the favorable glucose-responsive performances of this advanced nano-platform with a single intravenous injection maintaining blood glucose balance in Type 1 Diabetes(T1D)mice for an extended duration without the hypoglycemia risk.Therefore,this biomimetic insulin delivery system is poised to function as a strategy for the intravenous insulin administration,offering a promising drug candidate for the self-adaptive long-term T1D treatment.展开更多
文章基于三阶段SBM-DEA模型,提出了一个城市可持续发展效率(Urban Sustainable Development Efficiency,USDE)评价框架,并对2007-2017年中国粤港澳大湾区--香港澳门加内地九市的共11个城市的可持续发展效率进行分析。研究结论认为:所有...文章基于三阶段SBM-DEA模型,提出了一个城市可持续发展效率(Urban Sustainable Development Efficiency,USDE)评价框架,并对2007-2017年中国粤港澳大湾区--香港澳门加内地九市的共11个城市的可持续发展效率进行分析。研究结论认为:所有城市的效率在2007-2017年间,基本呈上升趋势。比较第一阶段和第三阶段结果,发现所有城市的USDE都发生了变化,表明外部环境变量对城市可持续发展效率的影响不可忽视;另外粤港澳大湾区整体发展效率比较高。其中澳门的效率平均值最大,也最稳定;深圳、香港、珠海、中山、广州、东莞和佛山为高等效率城市;江门、惠州和肇庆为中等效率城市。因此,应充分考虑影响城市发展效率的各相关因素,从而提出提升城市可持续发展效率的建议。展开更多
废弃电器电子产品(waste electrical and electronic equipment,WEEE)是重要的“城市矿产”之一,识别WEEE资源回收潜力及经济价值,可为澳门特区制定高效管理及循环处理政策提供基础数据及科学支撑。运用消费使用模型、市场供给模型,定...废弃电器电子产品(waste electrical and electronic equipment,WEEE)是重要的“城市矿产”之一,识别WEEE资源回收潜力及经济价值,可为澳门特区制定高效管理及循环处理政策提供基础数据及科学支撑。运用消费使用模型、市场供给模型,定量分析了2000—2050年澳门特区EEE(electrical and electronic equipment)的存量、废弃回收潜力及经济价值,结合澳门特区的WEEE回收及管理现状提出了相应的改善建议。研究表明,(1)随着澳门特区的发展,EEE的保有量不断增长,由2000年的325.2×10^(4)台增长到2050年的874.8×10^(4)台,年平均增长率为3%左右,增长高峰期出现在2011—2015年(15.6%~16.5%)。(2)澳门特区WEEE的产生量在2000—2014年呈超6%快速增长趋势,2015—2020增势减缓到约为4%,预计未来2021—2050年增长趋势趋向平稳,年产生总量维持在120×10^(4)台以上。废弃通讯和信息设备在所有WEEE中增速最快,2050年的报废量相比2000年增加了14.5倍。(3)澳门特区WEEE资源潜力自2014年起增势稳定,年产生量超过8000 t。其中,铁,铝,铜等基础金属贡献了总可回收资源量的70%以上。(4)WEEE可回收资源总经济价值超过4×10^(7)美元,大型家电、信息和通讯设备、多媒体设备占总回收价值的约99%;金和铜的可回收价值最高,占回收价值总量超过66%。(5)当前,澳门特区仅有10%的WEEE进入政府主导的正规回收企业,未来仍需要进一步改善回收现状。澳门特区WEEE进入快速报废阶段,但本地回收率及处理能力有待提升。识别澳门特区WEEE产生情况、加大WEEE回收力度、健全资源循环利用体系,有望缓解澳门特区废物处置压力,促进澳门特区绿色循环性社会建设。展开更多
This study presents a rapid and versatile low-cost sample-to-answer system for SARS-CoV-2 diagnostics.The system integrates the extraction and purification of nucleic acids,followed by amplification via either reverse...This study presents a rapid and versatile low-cost sample-to-answer system for SARS-CoV-2 diagnostics.The system integrates the extraction and purification of nucleic acids,followed by amplification via either reverse transcriptionquantitative polymerase chain reaction(RT–qPCR)or reverse transcription loop-mediated isothermal amplification(RT–LAMP).By meeting diverse diagnostic and reagent needs,the platform yields testing results that closely align with those of commercial RT-LAMP and RT‒qPCR systems.Notable advantages of our system include its speed and costeffectiveness.The assay is completed within 28 min,including sample loading(5 min),ribonucleic acid(RNA)extraction(3 min),and RT-LAMP(20 min).The cost of each assay is≈$9.5,and this pricing is competitive against that of Food and Drug Administration(FDA)-approved commercial alternatives.Although some RNA loss during on-chip extraction is observed,the platform maintains a potential limit of detection lower than 297 copies.Portability makes the system particularly useful in environments where centralized laboratories are either unavailable or inconveniently located.Another key feature is the platform’s versatility,allowing users to choose between RT‒qPCR or RT‒LAMP tests based on specific requirements.展开更多
基金National Natural Science Foundation of China(Nos.32171324 and 31971301)helped to support this work,Fundamental Research Funds for Central Universities(Nos.2024CDJXY017 and 2023CDJXY-051)Fundamental Research Funds for the National Key R&D Project(No.2022YFF0710700)+1 种基金2030 Major Research Projects for Technological Innovation(No.2023ZD0509402)Natural Science Foundation of Chongqing(Nos.2022NSCQ-BHX4850,CSTB2022NSCQ-BHX0718,and CSTB2022NSCQ-BHX0724).
文摘Diabetes,a prevalent chronic metabolic disorder,often leads to severe complications.Currently,existing treatment methods may pose life-threatening risks due to poor patient compliance and inaccurate dosing of subcutaneous insulin injections.Hence,a biomimetic red blood cell(RBC)membrane-coated glucose-responsive nanoplatform is developed for controlling insulin release.Functionalizing nanoplatforms with RBC membrane can prolong the half-life of nano-formulation in vivo mediated by the biomimetic immune escape.Simultaneously,the cascade catalytic effect of glucose oxidase(GOx)encapsulated in metal-organic frameworks(MOFs)and hemoglobin(Hb)in the RBC membrane are able to not only facilitate glucose-responsive insulin release,but also eliminate the detrimental by-product hydrogen peroxide(H_(2)O_(2))resulting from the Hb mediated H_(2)O_(2)scavenging.Both in vitro and in vivo studies have demonstrated the favorable glucose-responsive performances of this advanced nano-platform with a single intravenous injection maintaining blood glucose balance in Type 1 Diabetes(T1D)mice for an extended duration without the hypoglycemia risk.Therefore,this biomimetic insulin delivery system is poised to function as a strategy for the intravenous insulin administration,offering a promising drug candidate for the self-adaptive long-term T1D treatment.
文摘文章基于三阶段SBM-DEA模型,提出了一个城市可持续发展效率(Urban Sustainable Development Efficiency,USDE)评价框架,并对2007-2017年中国粤港澳大湾区--香港澳门加内地九市的共11个城市的可持续发展效率进行分析。研究结论认为:所有城市的效率在2007-2017年间,基本呈上升趋势。比较第一阶段和第三阶段结果,发现所有城市的USDE都发生了变化,表明外部环境变量对城市可持续发展效率的影响不可忽视;另外粤港澳大湾区整体发展效率比较高。其中澳门的效率平均值最大,也最稳定;深圳、香港、珠海、中山、广州、东莞和佛山为高等效率城市;江门、惠州和肇庆为中等效率城市。因此,应充分考虑影响城市发展效率的各相关因素,从而提出提升城市可持续发展效率的建议。
文摘废弃电器电子产品(waste electrical and electronic equipment,WEEE)是重要的“城市矿产”之一,识别WEEE资源回收潜力及经济价值,可为澳门特区制定高效管理及循环处理政策提供基础数据及科学支撑。运用消费使用模型、市场供给模型,定量分析了2000—2050年澳门特区EEE(electrical and electronic equipment)的存量、废弃回收潜力及经济价值,结合澳门特区的WEEE回收及管理现状提出了相应的改善建议。研究表明,(1)随着澳门特区的发展,EEE的保有量不断增长,由2000年的325.2×10^(4)台增长到2050年的874.8×10^(4)台,年平均增长率为3%左右,增长高峰期出现在2011—2015年(15.6%~16.5%)。(2)澳门特区WEEE的产生量在2000—2014年呈超6%快速增长趋势,2015—2020增势减缓到约为4%,预计未来2021—2050年增长趋势趋向平稳,年产生总量维持在120×10^(4)台以上。废弃通讯和信息设备在所有WEEE中增速最快,2050年的报废量相比2000年增加了14.5倍。(3)澳门特区WEEE资源潜力自2014年起增势稳定,年产生量超过8000 t。其中,铁,铝,铜等基础金属贡献了总可回收资源量的70%以上。(4)WEEE可回收资源总经济价值超过4×10^(7)美元,大型家电、信息和通讯设备、多媒体设备占总回收价值的约99%;金和铜的可回收价值最高,占回收价值总量超过66%。(5)当前,澳门特区仅有10%的WEEE进入政府主导的正规回收企业,未来仍需要进一步改善回收现状。澳门特区WEEE进入快速报废阶段,但本地回收率及处理能力有待提升。识别澳门特区WEEE产生情况、加大WEEE回收力度、健全资源循环利用体系,有望缓解澳门特区废物处置压力,促进澳门特区绿色循环性社会建设。
基金support of Grant No.52150710541 from the National Natural Science Foundation of China(NSFC),P.R.of ChinaGrant No.2018YFE0109000 from the Ministry of Science and Technology of the P.R.of China+2 种基金Petra Vopařilováand other members of Mendel University would like to acknowledge financial support from the Internal Grant Agency of the Faculty of AgriSciences,Mendel University in Brno(No.AF-IGA2023-IP-030)Haoqing Zhang would like to acknowledge the financial support of Grant No.2023-JC-QN-0130 from the Natural Science Basic Research Program of Shaanxi Province,P.R.ChinaGrant No.2023M732815 from the P.R.China Postdoctoral Science Foundation.
文摘This study presents a rapid and versatile low-cost sample-to-answer system for SARS-CoV-2 diagnostics.The system integrates the extraction and purification of nucleic acids,followed by amplification via either reverse transcriptionquantitative polymerase chain reaction(RT–qPCR)or reverse transcription loop-mediated isothermal amplification(RT–LAMP).By meeting diverse diagnostic and reagent needs,the platform yields testing results that closely align with those of commercial RT-LAMP and RT‒qPCR systems.Notable advantages of our system include its speed and costeffectiveness.The assay is completed within 28 min,including sample loading(5 min),ribonucleic acid(RNA)extraction(3 min),and RT-LAMP(20 min).The cost of each assay is≈$9.5,and this pricing is competitive against that of Food and Drug Administration(FDA)-approved commercial alternatives.Although some RNA loss during on-chip extraction is observed,the platform maintains a potential limit of detection lower than 297 copies.Portability makes the system particularly useful in environments where centralized laboratories are either unavailable or inconveniently located.Another key feature is the platform’s versatility,allowing users to choose between RT‒qPCR or RT‒LAMP tests based on specific requirements.
