The ruthenium oxide(RuO_x) thin film was prepared by using the R.F.sputtering system.The RuO_x pH-sensitive electrode possesses a nearly-Nernstian pH sensitivity(about 57 mV/pH).For the enzyme penicillinase was immobi...The ruthenium oxide(RuO_x) thin film was prepared by using the R.F.sputtering system.The RuO_x pH-sensitive electrode possesses a nearly-Nernstian pH sensitivity(about 57 mV/pH).For the enzyme penicillinase was immobilized directly onto a RuO_x pH-sensitive surface by 3-glycidoxypropyltrimethoxysilane(GPTS).Some characteristics of the biosensor, such as sensitivity,linear range,and drift,are investigated and the difference between penicillinase immobilized and without are compared.According to the time-constant model,we can extract the time constant and amplitude at the response curve of the drift.Subsequently,the extracted parameters were used to simulate the mechanism of the drift behavior.展开更多
文摘The ruthenium oxide(RuO_x) thin film was prepared by using the R.F.sputtering system.The RuO_x pH-sensitive electrode possesses a nearly-Nernstian pH sensitivity(about 57 mV/pH).For the enzyme penicillinase was immobilized directly onto a RuO_x pH-sensitive surface by 3-glycidoxypropyltrimethoxysilane(GPTS).Some characteristics of the biosensor, such as sensitivity,linear range,and drift,are investigated and the difference between penicillinase immobilized and without are compared.According to the time-constant model,we can extract the time constant and amplitude at the response curve of the drift.Subsequently,the extracted parameters were used to simulate the mechanism of the drift behavior.
