AIM： To detect the common intestinal pathogenic bacteria quickly and accurately.METHODS： A rapid （〈3 h） experimental procedure was set up based upon the gene chip technology, Target genes were amplified and hybri...AIM： To detect the common intestinal pathogenic bacteria quickly and accurately.METHODS： A rapid （〈3 h） experimental procedure was set up based upon the gene chip technology, Target genes were amplified and hybridized by oligonucleotide microarrays.RESULTS： One hundred and seventy strains of bacteria in pure culture belonging to 11 genera were successfully discriminated under comparatively same conditions, and a series of specific hybridization maps corresponding to each kind of bacteria were obtained. When this method was applied to 26 divided cultures, 25 （96.2%） were identified.CONCLUSION： Salmonella sp., Escherichia coli, Shigella sp., Listeria monocytogenes, Vibrio parahaemolyticus, Staphylococcus aureus , Proteus sp., Bacillus cereus, Vibrio cholerae, Enterococcus faecalis, Yersinia enterocolitica, and Campylobacter jejuni can be detected and identified by our microarrays. The accuracy, range, and discrimination power of this assay can be continually improved by adding further oligonudeotides to the arrays without any significant increase of complexity or cost.展开更多
基金Supported by the National High Technology ResearchDevelopment Program of China (863 Program), No.2002AA2Z2011
文摘AIM： To detect the common intestinal pathogenic bacteria quickly and accurately.METHODS： A rapid （〈3 h） experimental procedure was set up based upon the gene chip technology, Target genes were amplified and hybridized by oligonucleotide microarrays.RESULTS： One hundred and seventy strains of bacteria in pure culture belonging to 11 genera were successfully discriminated under comparatively same conditions, and a series of specific hybridization maps corresponding to each kind of bacteria were obtained. When this method was applied to 26 divided cultures, 25 （96.2%） were identified.CONCLUSION： Salmonella sp., Escherichia coli, Shigella sp., Listeria monocytogenes, Vibrio parahaemolyticus, Staphylococcus aureus , Proteus sp., Bacillus cereus, Vibrio cholerae, Enterococcus faecalis, Yersinia enterocolitica, and Campylobacter jejuni can be detected and identified by our microarrays. The accuracy, range, and discrimination power of this assay can be continually improved by adding further oligonudeotides to the arrays without any significant increase of complexity or cost.
