Most leguminous plants establish symbiotic relationships with rhizobia to form root organs called nodules(Ferguson et al.,2010).Nodules are specialized organs containing bacterial symbionts,which can provide enormous ...Most leguminous plants establish symbiotic relationships with rhizobia to form root organs called nodules(Ferguson et al.,2010).Nodules are specialized organs containing bacterial symbionts,which can provide enormous amounts of fixed nitrogen to their plant hosts(Peoples et al.,2009).Soybean(Glycine max),an economically important grain and oil crop,forms symbiotic nitrogen-fixing nodules,which reduces the demand for chemical nitrogen fertilizers and promotes yield(Saito et al.,2014).Nodule development is spatiotemporally regulated by the action of a number of transcription factors(TFs).展开更多
Coronavirus disease 2019(COVID‐19)has become a pandemic disease globally.Although COVID-19 directly invades lungs,it also involves the nervous system.Therefore,patients with nervous system involvement as the presenti...Coronavirus disease 2019(COVID‐19)has become a pandemic disease globally.Although COVID-19 directly invades lungs,it also involves the nervous system.Therefore,patients with nervous system involvement as the presenting symptoms in the early stage of infection may easily be misdiagnosed and their treatment delayed.They become silent contagious sources or‘virus spreaders’.In order to help neurologists to better understand the occurrence,development and prognosis,we have developed this consensus of prevention and management of COVID‐19.It can also assist other healthcare providers to be familiar with and recognise COVID-19 in their evaluation of patients in the clinic and hospital environment.展开更多
Coronavirus disease-2019(COVID‐19)has become a global pandemic.COVID-19 runs its course in two phases,the initial incubation phase and later clinical symptomatic phase.Patients in the initial incubation phase often h...Coronavirus disease-2019(COVID‐19)has become a global pandemic.COVID-19 runs its course in two phases,the initial incubation phase and later clinical symptomatic phase.Patients in the initial incubation phase often have insidious clinical symptoms,but they are still highly contagious.At the later clinical symptomatic phase,the immune system is fully activated and the disease may enter the severe infection stage in this phase.Although many patients are known for their respiratory symptoms,they had neurological symptoms in their first 1-2 days of clinical symptomatic phase,and ischaemic stroke occurred 2 weeks after the onset of the clinical symptomatic phase.The key is to prevent a patient from progressing to this severe infection from mild infection.We are sharing our experience on prevention and management of COVID-19.展开更多
Native promoters that can drive high and stable transgene expression are important tools for modifying plant traits.Although several such promoters have been reported in soybean(Glycine max),few of them function at mu...Native promoters that can drive high and stable transgene expression are important tools for modifying plant traits.Although several such promoters have been reported in soybean(Glycine max),few of them function at multiple growth and development stages and during nodule development.Here,we report that the promoters of 40S RIBOSOMAL PROTEIN SMALL SUBUNIT S28(RPS28)and EUKARYOTIC TRANSLATION INITIATION FACTOR 1(EIF1)are ideal for high expression of transgene.Through bioinformatic analysis,we determined that RPS28 and EIF1 were highly expressed during soybean growth and development,nodule development,and various biotic and abiotic stresses.Fusion of both RPS28 and EIF1 promoters,with or without their first intron,with the reporter geneβ-GLUCURONIDASE(uidA)in transgenic soybean,resulted in high GUS activity in seedlings,seeds,and nodules.Fluorimetric GUS assays showed that the RPS28 promoter and the EIF1 promoter yielded high expression,comparable to the soybean Ubiquitin(GmUbi)promoter.RPS28 and EIF1 promoters were also highly expressed in Arabidopsis thaliana and Nicotiana benthamiana.Our results indicate the potential of RPS28 and EIF1 promoters to facilitate future genetic engineering and breeding to improve the quality and yield of soybean,as well as in a wide variety of other plant species.展开更多
基金supported by grants from the National Key Research and Development Program(2022YFA0912100 to X.W.,S.S.)the National Natural Science Foundation of China(U21A20181 to X.W.)+2 种基金the Outstanding Talents Fund of Henan University of China(CX3050A092004 to X.W.)the Zhongyuan Scholar of Henan Province(224000510001 to X.W.)the Program for Innovative Research Team(in Science and Technology)in University of Henan Province(23IRTSTHN020 to S.S.).
文摘Most leguminous plants establish symbiotic relationships with rhizobia to form root organs called nodules(Ferguson et al.,2010).Nodules are specialized organs containing bacterial symbionts,which can provide enormous amounts of fixed nitrogen to their plant hosts(Peoples et al.,2009).Soybean(Glycine max),an economically important grain and oil crop,forms symbiotic nitrogen-fixing nodules,which reduces the demand for chemical nitrogen fertilizers and promotes yield(Saito et al.,2014).Nodule development is spatiotemporally regulated by the action of a number of transcription factors(TFs).
基金supported by the National Key Research and Development Program of China(No.2018YFC1312200 to BH)the National Natural Science Foundation of China(No.81820108010 to BH,No.81974182 to LM and No.81671147 to HJ)major refractory diseases pilot project of clinical collaboration with Chinese and Western Medicine(SATCM-20180339).
文摘Coronavirus disease 2019(COVID‐19)has become a pandemic disease globally.Although COVID-19 directly invades lungs,it also involves the nervous system.Therefore,patients with nervous system involvement as the presenting symptoms in the early stage of infection may easily be misdiagnosed and their treatment delayed.They become silent contagious sources or‘virus spreaders’.In order to help neurologists to better understand the occurrence,development and prognosis,we have developed this consensus of prevention and management of COVID‐19.It can also assist other healthcare providers to be familiar with and recognise COVID-19 in their evaluation of patients in the clinic and hospital environment.
基金supported by the National Key Research and Development Program of China(No.2018YFC1312200 to BH)the National Natural Science Foundation of China(No.81820108010 to BH).
文摘Coronavirus disease-2019(COVID‐19)has become a global pandemic.COVID-19 runs its course in two phases,the initial incubation phase and later clinical symptomatic phase.Patients in the initial incubation phase often have insidious clinical symptoms,but they are still highly contagious.At the later clinical symptomatic phase,the immune system is fully activated and the disease may enter the severe infection stage in this phase.Although many patients are known for their respiratory symptoms,they had neurological symptoms in their first 1-2 days of clinical symptomatic phase,and ischaemic stroke occurred 2 weeks after the onset of the clinical symptomatic phase.The key is to prevent a patient from progressing to this severe infection from mild infection.We are sharing our experience on prevention and management of COVID-19.
基金supported by National Natural Science Foundationof China(Grant No.31870257 and U21A20181 to X.W,Grant no.32170728 to H.W.)the National Key Research,and Development,Program(Grant No.2018YFE0112100 to X.W.)+1 种基金Excellent Youth Foundation of Henan Province(Grant No.222300420025 to H.W.)the 111 Project of China(Grant No.D16014).
文摘Native promoters that can drive high and stable transgene expression are important tools for modifying plant traits.Although several such promoters have been reported in soybean(Glycine max),few of them function at multiple growth and development stages and during nodule development.Here,we report that the promoters of 40S RIBOSOMAL PROTEIN SMALL SUBUNIT S28(RPS28)and EUKARYOTIC TRANSLATION INITIATION FACTOR 1(EIF1)are ideal for high expression of transgene.Through bioinformatic analysis,we determined that RPS28 and EIF1 were highly expressed during soybean growth and development,nodule development,and various biotic and abiotic stresses.Fusion of both RPS28 and EIF1 promoters,with or without their first intron,with the reporter geneβ-GLUCURONIDASE(uidA)in transgenic soybean,resulted in high GUS activity in seedlings,seeds,and nodules.Fluorimetric GUS assays showed that the RPS28 promoter and the EIF1 promoter yielded high expression,comparable to the soybean Ubiquitin(GmUbi)promoter.RPS28 and EIF1 promoters were also highly expressed in Arabidopsis thaliana and Nicotiana benthamiana.Our results indicate the potential of RPS28 and EIF1 promoters to facilitate future genetic engineering and breeding to improve the quality and yield of soybean,as well as in a wide variety of other plant species.