Using newly developed methods and software, association mapping was conducted for chromium content and total sugar in tobacco leaf, based on four-omics datasets. Our objective was to collect data on genotype and pheno...Using newly developed methods and software, association mapping was conducted for chromium content and total sugar in tobacco leaf, based on four-omics datasets. Our objective was to collect data on genotype and phenotype for 60 leaf samples at four developmental stages, from three plant architectural positions and for three cultivars that were grown in two locations. Association mapping was conducted to detect genetic variants at quantitative trait SNP(QTS) loci, quantitative trait transcript(QTT) differences,quantitative trait protein(QTP) variability, and quantitative trait metabolite(QTM) changes,which can be summarized as QTX locus variation. The total heritabilities of the four-omics loci for both traits tested were 23.60% for epistasis and 15.26% for treatment interaction.Epistasis and environment × treatment interaction had important impacts on complex traits at all-omics levels. For decreasing chromium content and increasing total sugar in tobacco leaf, six methylated loci can be directly used for marker-assisted selection, and expression of ten QTTs, seven QTPs and six QTMs can be modified by selection or cultivation.展开更多
In order to improve the curing quality and industrial availability of upper tobacco leaves,the effects of different temperature differences between dry and wet bulbs in yellowing stage on the chemical composition,arom...In order to improve the curing quality and industrial availability of upper tobacco leaves,the effects of different temperature differences between dry and wet bulbs in yellowing stage on the chemical composition,aroma substances and economic characters of upper tobacco leaves were investigated using field test and indoor test. The results showed that when the dry bulb temperature was 38℃ and the temperature difference between dry and wet bulbs was 1. 5℃,the coordination of chemical constituents and the content of aroma substances in the upper tobacco leaves were increased,the appearance quality and sensory evaluation quality were significantly improved,and the economic characters after curing were improved,thereby improving the yield and quality of the upper tobacco leaves.展开更多
基金supported by the National Basic Research Program of China (2011CB109306 and 2009CB118404)the Program of Introducing Talents of Discipline to Universities of China ("111" Project, B06014)Research Programs (CNTC-D2011100, CNTC-[2012]146, NY-[2011]3047, QKHRZ [2013] 02)
文摘Using newly developed methods and software, association mapping was conducted for chromium content and total sugar in tobacco leaf, based on four-omics datasets. Our objective was to collect data on genotype and phenotype for 60 leaf samples at four developmental stages, from three plant architectural positions and for three cultivars that were grown in two locations. Association mapping was conducted to detect genetic variants at quantitative trait SNP(QTS) loci, quantitative trait transcript(QTT) differences,quantitative trait protein(QTP) variability, and quantitative trait metabolite(QTM) changes,which can be summarized as QTX locus variation. The total heritabilities of the four-omics loci for both traits tested were 23.60% for epistasis and 15.26% for treatment interaction.Epistasis and environment × treatment interaction had important impacts on complex traits at all-omics levels. For decreasing chromium content and increasing total sugar in tobacco leaf, six methylated loci can be directly used for marker-assisted selection, and expression of ten QTTs, seven QTPs and six QTMs can be modified by selection or cultivation.
文摘In order to improve the curing quality and industrial availability of upper tobacco leaves,the effects of different temperature differences between dry and wet bulbs in yellowing stage on the chemical composition,aroma substances and economic characters of upper tobacco leaves were investigated using field test and indoor test. The results showed that when the dry bulb temperature was 38℃ and the temperature difference between dry and wet bulbs was 1. 5℃,the coordination of chemical constituents and the content of aroma substances in the upper tobacco leaves were increased,the appearance quality and sensory evaluation quality were significantly improved,and the economic characters after curing were improved,thereby improving the yield and quality of the upper tobacco leaves.
