Maize(Zea mays)is one of the most important crops in the world,but its yield and quality are seriously affected by diverse diseases.Identifying broad-spectrum resistance genes is crucial for developing effective strat...Maize(Zea mays)is one of the most important crops in the world,but its yield and quality are seriously affected by diverse diseases.Identifying broad-spectrum resistance genes is crucial for developing effective strategies to control the disease in maize.In a genome-wide study in maize,we identified a G-type lectin receptor kinase ZmLecRK1,as a new resistance protein against Pythium aphanidermatum,one of the causal pathogens of stalk rot in maize.Genetic analysis showed that the specific ZmLecRK1 allele can confer resistance to multiple pathogens in maize.The cell death and disease resistance phenotype mediated by the resistant variant of ZmLecRK1 requires the co-receptor ZmBAK1.A naturally occurring A404S variant in the extracellular domain of ZmLecRK1 determines the ZmLecRK1-ZmBAK1 interaction and the formation of ZmLecRK1-related protein complexes.Interestingly,the ZmLecRK1 susceptible variant was found to possess the amino acid S404 that is present in the ancestral variants of ZmLecRK1 and conserved among the majority of grass species,while the resistance variant of ZmLecRK1 with A404 is only present in a few maize inbred lines.Substitution of S by A at position 404 in ZmLecRK1-like proteins of sorghum and rice greatly enhances their ability to induce cell death.Further transcriptomic analysis reveals that ZmLecRK1 likely regulates gene expression related to the pathways in cell wall organization or biogenesis in response to pathogen infection.Taken together,these results suggest that the ZmLecRK1 resistance variant enhances its binding affinity to the co-receptor ZmBAK1,thereby enhancing the formation of active complexes for defense in maize.Our work highlights the biotechnological potential for generating disease-resistant crops by precisely modulating the activity of ZmLecRK1 and its homologs through targeted base editing.展开更多
基金supported by Biological Breeding-National Science and Technology Major Project(no.2023ZD04070,W.Z.)the National Key Research and Development Program,Ministry of Science and Technology of China(no.2022YFD1201802,W.Z.)+1 种基金the National Natural Science Foundation of China(no.32472499,W.Z.)the Pinduoduo-China Agricultural University Research Fund(no.PC2023A01005,Y.-L.P.).
文摘Maize(Zea mays)is one of the most important crops in the world,but its yield and quality are seriously affected by diverse diseases.Identifying broad-spectrum resistance genes is crucial for developing effective strategies to control the disease in maize.In a genome-wide study in maize,we identified a G-type lectin receptor kinase ZmLecRK1,as a new resistance protein against Pythium aphanidermatum,one of the causal pathogens of stalk rot in maize.Genetic analysis showed that the specific ZmLecRK1 allele can confer resistance to multiple pathogens in maize.The cell death and disease resistance phenotype mediated by the resistant variant of ZmLecRK1 requires the co-receptor ZmBAK1.A naturally occurring A404S variant in the extracellular domain of ZmLecRK1 determines the ZmLecRK1-ZmBAK1 interaction and the formation of ZmLecRK1-related protein complexes.Interestingly,the ZmLecRK1 susceptible variant was found to possess the amino acid S404 that is present in the ancestral variants of ZmLecRK1 and conserved among the majority of grass species,while the resistance variant of ZmLecRK1 with A404 is only present in a few maize inbred lines.Substitution of S by A at position 404 in ZmLecRK1-like proteins of sorghum and rice greatly enhances their ability to induce cell death.Further transcriptomic analysis reveals that ZmLecRK1 likely regulates gene expression related to the pathways in cell wall organization or biogenesis in response to pathogen infection.Taken together,these results suggest that the ZmLecRK1 resistance variant enhances its binding affinity to the co-receptor ZmBAK1,thereby enhancing the formation of active complexes for defense in maize.Our work highlights the biotechnological potential for generating disease-resistant crops by precisely modulating the activity of ZmLecRK1 and its homologs through targeted base editing.
