Cotton fibers are single cells originating in the epidermis of cotton ovules,and serve as the largest natural fiber source for the textile industry.In theory,all epidermal cells have the potential to develop into fibe...Cotton fibers are single cells originating in the epidermis of cotton ovules,and serve as the largest natural fiber source for the textile industry.In theory,all epidermal cells have the potential to develop into fibers,but only 15%–25%of epidermis cells develop into commercially viable lint fibers.We previously showed that Gh Lac1 participates in cotton defense against biotic stress.Here we report that Gh Lac1 also has a role in cotton fiber development.Gh Lac1 RNAi lines in cotton showed increased differentiation of fiber initials from epidermis and shortened fiber length,resulting in unchanged lint percentage.Suppression of Gh Lac1 expression led to constitutively hyperaccumulated jasmonic acid(JA)and flavonoids in ovules and fiber cells.In vitro ovule culture experiments confirmed the distinct roles of JA and flavonoids in fiber initiation and elongation,and showed that fiber development is spatially regulated by these chemicals:the increased fiber initiation in Gh Lac1 RNAi lines is caused by hyperaccumulated JA and rutin content during the fiber initiation stage and shortened fiber length is caused by constitutively increased JA and naringenin content during the fiber elongation stage.展开更多
基金financially supported by the National Transgenic Plant Research Program of China(2016ZX08005-001)the Program of Introducing Talents of Discipline to Universities in China(B14032)+1 种基金the open funds of the National Key Laboratory of Crop Genetic Improvement(ZK201901)the National Natural Science Foundation of China(31771837)。
文摘Cotton fibers are single cells originating in the epidermis of cotton ovules,and serve as the largest natural fiber source for the textile industry.In theory,all epidermal cells have the potential to develop into fibers,but only 15%–25%of epidermis cells develop into commercially viable lint fibers.We previously showed that Gh Lac1 participates in cotton defense against biotic stress.Here we report that Gh Lac1 also has a role in cotton fiber development.Gh Lac1 RNAi lines in cotton showed increased differentiation of fiber initials from epidermis and shortened fiber length,resulting in unchanged lint percentage.Suppression of Gh Lac1 expression led to constitutively hyperaccumulated jasmonic acid(JA)and flavonoids in ovules and fiber cells.In vitro ovule culture experiments confirmed the distinct roles of JA and flavonoids in fiber initiation and elongation,and showed that fiber development is spatially regulated by these chemicals:the increased fiber initiation in Gh Lac1 RNAi lines is caused by hyperaccumulated JA and rutin content during the fiber initiation stage and shortened fiber length is caused by constitutively increased JA and naringenin content during the fiber elongation stage.
