Pharmacokinetics of different dosage of astragalus membranaceus of buyang huanwu decoction in rats with cerebral ischemic injury by microdialysis combined with LC/MS

To investigate the pharmacokinetics of effective components of Bu Yang Huang Wu Decoction(BYHWD)with different dosages of Astragalus(Yiqi groups and Huoxue groups)applicating in rats with middle cerebral artery occlusion(MCAO).Replicating the animal model of cerebral ischemia-reperfusion in rats,establishing the liquid-mass spectrometry method for the determination of BYHWD and researching the pharmacokinetics of effective components of yiqi groups of BYHWD with different dosages of astragalus(3.09,6.17,12.34 g/Kg)and Huoxue goups(2.32 g/Kg)when applicated seperately in the rats suffering from cerebral ischemia reperfusion injury after femoral vein administration.The pharmacokinetics of formononetin and paeoniflorin in the different dosage groups of BYHWD met the one-compartment model,and the t1/2 of formononetin and paeoniflorin in the low-dose Yiqi and Huoxue groups were(88.43±3.82,69.18±0.11)min,MRT were(138.56±4.83,113.62±2.42)min,and AUC0-twere(28488.35±4800.32,140614.80±23954.05)ng/mL min;The t1/2 of formononetin and paeoniflorin in the middle-dose Yiqi group and Huoxue group were(82.16±1.78,67.08±3.69)min,and MRT were(127.95±2.70,116.58±4.13),AUC0-t were(48619.25±6745.75,159026.00±15003.33)ng/mL min;The t1/2 of formononetin and paeoniflorin in the high-dose Yiqi and Huoxue groups were(80.29±1.12,69.69±0.87)min and MRT was(128.79±1.46,118.78±4.56)min AUC0-twere(109942.90±13101.83,189417.90±22311.00)ng/mL·min.The concentration rate of formononetin t1/2 brain was decreased with increase of Astragalus dose.However,no significant difference between these two variables was found during experiments.Furthermore,the experiments showed that the increasing dose of astragalus would affect the pharmacokinetic behavior of paeoniflorin in the Huoxue groups.More specifically,the result showed that paeoniflorin can be metabolized more slowly in the body when applicated in high dose of the jaundice administration groups.In this way,the effect of paeoniflorin can be lasted for longer time in the body and brain.