Hydralazine represses Fpn ubiquitination to rescue injured neurons via competitive binding to UBA52

A major impedance to neuronal regeneration after peripheral nerve injury (PNI) is the activation of various programmed cell death mechanisms in the dorsal root ganglion. Ferroptosis is a form of programmed cell death distinguished by imbalance in iron and thiol metabolism, leading to lethal lipid peroxidation. However, the molecular mechanisms of ferroptosis in the context of PNI and nerve regeneration remain unclear. Ferroportin (Fpn), the only known mammalian nonheme iron export protein, plays a pivotal part in inhibiting ferroptosis by maintaining intracellular iron homeostasis. Here, we explored in vitro and in vivo the involvement of Fpn in neuronal ferroptosis. We first delineated that reactive oxygen species at the injury site induces neuronal ferroptosis by increasing intracellular iron via accelerated UBA52-driven ubiquitination and degradation of Fpn, and stimulation of lipid peroxidation. Early administration of the potent arterial vasodilator, hydralazine (HYD), decreases the ubiquitination of Fpn after PNI by binding to UBA52, leading to suppression of neuronal cell death and significant acceleration of axon regeneration and motor function recovery. HYD targeting of ferroptosis is a promising strategy for clinical management of PNI.

Identification and fine mapping of qSW2 for leaf slow wilting in soybean

Drought is one of the abiotic stresses limiting the production of soybean(Glycine max).Elucidation of the genetic and molecular basis of the slow-wilting(SW)trait of this crop offers the prospect of its genetic improvement.A panel of 188 accessions and a set of recombinant inbred lines produced from a cross between cultivars Liaodou 14 and Liaodou 21 were used to identify quantitative-trait loci(QTL)associated with SW.Plants were genotyped by Specific-locus amplified fragment sequencing and seedling leaf wilting was assessed under three water-stress treatments.A genome-wide association study identified 26 SW-associated single-nucleotide polymorphisms(SNPs),including three located in a 248-kb linkage-disequilibrium(LD)block on chromosome 2.Linkage mapping revealed a major-effect QTL,qSW2,associated with all three treatments and adjacent to the LD block.Fine mapping in a BC_(2)F_(3) population derived from a backcross between Liaodou 21 and R26 confined qSW2 to a 60-kb interval.Gene expression and sequence variation analysis identified the gene Glyma.02 g218100,encoding an auxin transcription factor,as a candidate gene for qSW2.Our results will contribute significantly to improving drought-resistant soybean cultivars by providing genetic information and resources.

用于柔性智能变色玻璃的叶脉状分级银网络透明电极

呈现出可逆颜色变化的智能窗户可调节阳光的透射,节省大量的能耗.目前,在众多智能玻璃中,柔性电致变色器件由于可实现卷对卷生产,良好的控制性以及与其他能源(太阳能电池和锂电池)的兼容性而受到广泛关注.因此,作为柔性电致变色器件不可或缺的部分,柔性透明电极变得越来越重要.本文基于叶脉状的分层金属网络构造了大面积的柔性透明电极,该多级网络由介观尺度的"树干"和微观尺度的"分支"组成.自发形成的"分支"使导电路径均匀分布,而激光蚀刻的"树干"则能够将收集的电子进行远距离传输.该透明电极表现出高的透光率(~81%)和低的电阻(1.36Ωsq–1),并可通过调整网格的宽度、边长和裂纹图案的大小来优化性能.在此基础上,制造了具有显著循环性能的柔性电致变色器件.具有高透明度、导电性和柔韧性的多级银网络透明电极未来将可能在下一代柔性可穿戴光电设备中有广泛的应用前景.