Fiber productivity and quality of cotton are severely affected by abiotic stresses.In this study,we identified the role of GhADF1,an actin depolymerizing factor,in cotton response to drought stress.GhADF1 expression i...Fiber productivity and quality of cotton are severely affected by abiotic stresses.In this study,we identified the role of GhADF1,an actin depolymerizing factor,in cotton response to drought stress.GhADF1 expression in cotton could be induced by PEG6000.GhADF1-RNAi transgenic cotton showed increased tolerance to drought stress during seed germination and seedling development as well as at the reproductive stage.In contrast,overexpression of GhADF1 led to a drought-sensitive phenotype in transgenic plants.GhADF1-RNAi plants produced an enlarged root system with longer primary roots,more lateral roots,increased root dry biomass,and increased cell size.In leaves of GhADF1-RNAi cotton,proline content and activities of reactive oxygen species-scavenging enzymes were increased following drought stress compared with those in wild type.GhADF1-RNAi lines showed higher water-use efficiency than the wild type,accompanied by reduced leaf stomatal density and conductance.GhADF1-RNAi cotton produced higher fiber yield in the field under both normal and drought conditions.Transcriptomic analyses identified 124 differentially expressed genes in leaves of GhADF1-RNAi lines compared with the wild type following drought treatment.Upregulated genes included those encoding transcription factors,protein kinases,heat shock proteins,and other proteins known to be involved in stress responses.We conclude that GhADF1 reduces the expression of abiotic stress-associated genes in cotton response to drought stress and may be a promising candidate gene for crop improvement by genetic manipulation.展开更多
Dear Editor Verticillium wilt caused by Verticillium dahliae Kleb. is the most destructive disease in cotton-growing areas around the world. The infection significantly reduces cotton yield and fiber quality due to le...Dear Editor Verticillium wilt caused by Verticillium dahliae Kleb. is the most destructive disease in cotton-growing areas around the world. The infection significantly reduces cotton yield and fiber quality due to leaf chlorosis, necrosis or wilting, leaf or boll abscission, and even plant death. The losses may reach up to 80% of lint cotton yield （Wei et al., 2015）.展开更多
基金supported by the National Natural Science Foundation of China(31601350)the Project of Transgenic Research from the Ministry of Science and Technology of China(2016ZX08005-004-007)+1 种基金the Fundamental Research Project of Shanxi Province(20210302123381)the Science and Technology Innovation Project of Higher Education Institutions of Shanxi Province(2021L115).
文摘Fiber productivity and quality of cotton are severely affected by abiotic stresses.In this study,we identified the role of GhADF1,an actin depolymerizing factor,in cotton response to drought stress.GhADF1 expression in cotton could be induced by PEG6000.GhADF1-RNAi transgenic cotton showed increased tolerance to drought stress during seed germination and seedling development as well as at the reproductive stage.In contrast,overexpression of GhADF1 led to a drought-sensitive phenotype in transgenic plants.GhADF1-RNAi plants produced an enlarged root system with longer primary roots,more lateral roots,increased root dry biomass,and increased cell size.In leaves of GhADF1-RNAi cotton,proline content and activities of reactive oxygen species-scavenging enzymes were increased following drought stress compared with those in wild type.GhADF1-RNAi lines showed higher water-use efficiency than the wild type,accompanied by reduced leaf stomatal density and conductance.GhADF1-RNAi cotton produced higher fiber yield in the field under both normal and drought conditions.Transcriptomic analyses identified 124 differentially expressed genes in leaves of GhADF1-RNAi lines compared with the wild type following drought treatment.Upregulated genes included those encoding transcription factors,protein kinases,heat shock proteins,and other proteins known to be involved in stress responses.We conclude that GhADF1 reduces the expression of abiotic stress-associated genes in cotton response to drought stress and may be a promising candidate gene for crop improvement by genetic manipulation.
基金This work was supported by the grants from the Ministry of Agriculture of China （2008ZX08005-004 and 2011ZX08005-004-008） and the National Natural Science Foundation of China （31201182）.ACKNOWLEDGMENTS We are grateful to Prof. Gary. J. Loake （Edinburgh University, UK） and Dr. Hazel McLellan （Dundee University, UK） for their critical reading of the manuscript, Prof. Huishan Guo （institute of Microbiology, CAS） for kindly providing the V. dahliae V592 isolate. No conflict of interest declared.
文摘Dear Editor Verticillium wilt caused by Verticillium dahliae Kleb. is the most destructive disease in cotton-growing areas around the world. The infection significantly reduces cotton yield and fiber quality due to leaf chlorosis, necrosis or wilting, leaf or boll abscission, and even plant death. The losses may reach up to 80% of lint cotton yield （Wei et al., 2015）.
