Objective: To establish an orthotopic bladder cancer model bearing human bladder cancer for experimental research, and monitor tumor progression by magnetic resonance imaging (MRI). Methods: The mucosa was mechanicall...Objective: To establish an orthotopic bladder cancer model bearing human bladder cancer for experimental research, and monitor tumor progression by magnetic resonance imaging (MRI). Methods: The mucosa was mechanically damaged transurethrally under direct vision, and then human bladder cancer cell line T24 was inoculated into the bladders of BALB/c nude mice to establish orthotopic bladder cancer model. To find a suitable concentration of Gd-DTPA for this re- search. MRI was performed weekly to assess tumor growth, using Gd-DTPA as contrast agent. The pathologic morphology of the bladders and other specimens were observed with HE stain. Results: All the 25 mice developed bladder cancer after inoculation. The best concentration of Gd-DTPA was 1.408 mg/mL. On MRI, no change in the bladders was observed on day 7 after inoculation, filling defect in the bladders, accordant to actual tumor size, was detected on days 14, 21 and 28. Pathologic examination showed that tumor grew in the mucosa or superficial muscle of bladder on day 7, confined in muscle layer on days 14–28, and invaded serosa on day 35. Conclusion: Transurethrally damaged bladder mucosa under direct vision and instilled bladder cancer cell T24, we successfully established an orthotopic bladder cancer model. Tumor growth simulated the progression of human bladder cancer approximately. MRI was a reliable way for dynamic detection of murine orthotopic bladder tumor.展开更多
Male infertility, as a major issue of human reproduction health, prevents successful natural conception. Asthenoteratospermia mainly presents one or multiple anomalies in head, neck and tail of spermatozoa, and impair...Male infertility, as a major issue of human reproduction health, prevents successful natural conception. Asthenoteratospermia mainly presents one or multiple anomalies in head, neck and tail of spermatozoa, and impairs sperm function and motility (Coutton et al., 2015). Recurrent abnormalities of the fibrous sheath lead to multiple morphological abnormaliries of the sperm flagella (MMAF), which is a quite frequent type of asthenoteratospermia in male infertility (Chemes et al., 1987;Ben Khelifa et al, 2014).展开更多
基金Supported by the grants from the Talented Scholar Foundation of Jiangsu Province (No. RC2003094)the Sci-Tech research Foundation of Jiangsu Province (No. H200517)"The Six Talent Peak" Project Foundation of Jiangsu Province (06-B-021)
文摘Objective: To establish an orthotopic bladder cancer model bearing human bladder cancer for experimental research, and monitor tumor progression by magnetic resonance imaging (MRI). Methods: The mucosa was mechanically damaged transurethrally under direct vision, and then human bladder cancer cell line T24 was inoculated into the bladders of BALB/c nude mice to establish orthotopic bladder cancer model. To find a suitable concentration of Gd-DTPA for this re- search. MRI was performed weekly to assess tumor growth, using Gd-DTPA as contrast agent. The pathologic morphology of the bladders and other specimens were observed with HE stain. Results: All the 25 mice developed bladder cancer after inoculation. The best concentration of Gd-DTPA was 1.408 mg/mL. On MRI, no change in the bladders was observed on day 7 after inoculation, filling defect in the bladders, accordant to actual tumor size, was detected on days 14, 21 and 28. Pathologic examination showed that tumor grew in the mucosa or superficial muscle of bladder on day 7, confined in muscle layer on days 14–28, and invaded serosa on day 35. Conclusion: Transurethrally damaged bladder mucosa under direct vision and instilled bladder cancer cell T24, we successfully established an orthotopic bladder cancer model. Tumor growth simulated the progression of human bladder cancer approximately. MRI was a reliable way for dynamic detection of murine orthotopic bladder tumor.
基金supported by National Natural Science Foundation of China (31625015 and 31521003)Foundation of the Education Department of Anhui Province (KJ2016A370)+1 种基金Foundation of the Department of Science and Technology of Anhui Province (2017070802D150)Shanghai Municipal Science and Technology Major Project (2017SHZDZX01)
文摘Male infertility, as a major issue of human reproduction health, prevents successful natural conception. Asthenoteratospermia mainly presents one or multiple anomalies in head, neck and tail of spermatozoa, and impairs sperm function and motility (Coutton et al., 2015). Recurrent abnormalities of the fibrous sheath lead to multiple morphological abnormaliries of the sperm flagella (MMAF), which is a quite frequent type of asthenoteratospermia in male infertility (Chemes et al., 1987;Ben Khelifa et al, 2014).
