Objective:To present results of virological study of wild birds inhabiting Western Mongolia. Methods:Over a period of 2003-2008,we isolated 13 influenza A viruses:H1N1,H3N6, H13N8 and H4N6 subtypes.We did not isolate ...Objective:To present results of virological study of wild birds inhabiting Western Mongolia. Methods:Over a period of 2003-2008,we isolated 13 influenza A viruses:H1N1,H3N6, H13N8 and H4N6 subtypes.We did not isolate any H5N1 subtype,that still cause epizooty in wild birds and poultry.Results:We revealed taxonomic and ecological heterogeneity of the birds involved in maintenance of circulation of influenza viruses in the given territory.Influenza viruses were isolated from birds of 6 orders;among them there are species preferring water and semi-aquatic biotopes,one species preferring dry plain region,and also one species which can inhabit both dry and water biotopes.Conclusions:Representatives of all main orders of Western Mongolia avifauna are involved in support of influenza A virus circulation,highly pathogenic H5N1 influenza viruses were registered in Mongolia thus it’s necessary to continue permanent influenza virus surveillance in wild birds’ populations.展开更多
Objective:To evaluate the use of Real-Time PCR system based on specific amplification of matrix protein gene fragment for influenza A virus RNA detection in cloacal swabs from wild birds.Methods:Sensitivity,specificit...Objective:To evaluate the use of Real-Time PCR system based on specific amplification of matrix protein gene fragment for influenza A virus RNA detection in cloacal swabs from wild birds.Methods:Sensitivity,specificity and reproducibility of analysis results were identified. Study of cloacal swabs from wild birds for influenza A virus presence was performed.Results: Reproducibility of low concentrations of virus detection in samples by Real-Time PCR was significantly higher than that of detection based on cytopathic effect of viruses grown on MDCK cell culture.Conclusions:Real-Time PCR system for influenza A virus RNA detection is developed and applied for virus surveillance study.展开更多
文摘Objective:To present results of virological study of wild birds inhabiting Western Mongolia. Methods:Over a period of 2003-2008,we isolated 13 influenza A viruses:H1N1,H3N6, H13N8 and H4N6 subtypes.We did not isolate any H5N1 subtype,that still cause epizooty in wild birds and poultry.Results:We revealed taxonomic and ecological heterogeneity of the birds involved in maintenance of circulation of influenza viruses in the given territory.Influenza viruses were isolated from birds of 6 orders;among them there are species preferring water and semi-aquatic biotopes,one species preferring dry plain region,and also one species which can inhabit both dry and water biotopes.Conclusions:Representatives of all main orders of Western Mongolia avifauna are involved in support of influenza A virus circulation,highly pathogenic H5N1 influenza viruses were registered in Mongolia thus it’s necessary to continue permanent influenza virus surveillance in wild birds’ populations.
文摘Objective:To evaluate the use of Real-Time PCR system based on specific amplification of matrix protein gene fragment for influenza A virus RNA detection in cloacal swabs from wild birds.Methods:Sensitivity,specificity and reproducibility of analysis results were identified. Study of cloacal swabs from wild birds for influenza A virus presence was performed.Results: Reproducibility of low concentrations of virus detection in samples by Real-Time PCR was significantly higher than that of detection based on cytopathic effect of viruses grown on MDCK cell culture.Conclusions:Real-Time PCR system for influenza A virus RNA detection is developed and applied for virus surveillance study.
