INTRODUCTIONThe treatment of human epithelial malignancies islimited by drug resistance and toxic and side effects,which results in the failure in the treatment ofmajority of advanced cancer victims.To seek for anew,a...INTRODUCTIONThe treatment of human epithelial malignancies islimited by drug resistance and toxic and side effects,which results in the failure in the treatment ofmajority of advanced cancer victims.To seek for anew,and specific antineoplastic therapy willprovide hope for tumor treatment.Althoughdisordered intermediary metabolism in cancer cellshas been known for many years,much of the展开更多
AIM To investigate the application of confocallaser scanning microscopy(CLSM)in tumorpathology and three-dimensional( 3-D )reconstruction by CLSM in pathologic specimensof hepatocellular carcinoma(HCC).METHODS The 30...AIM To investigate the application of confocallaser scanning microscopy(CLSM)in tumorpathology and three-dimensional( 3-D )reconstruction by CLSM in pathologic specimensof hepatocellular carcinoma(HCC).METHODS The 30μm thick sections were cutfrom the paraffin-embedded tissues of HCC,hyperplasia and normal liver,stained with DNAfluorescent probe YOYO-1 iodide and examinedby CLSM to collect optical sections of nuclei and3-D images reconstructed.RESULTS HCC displayed chaotic arrangementof carcinoma cell nuclei,marked pleomorphism,indented and irregular nuclear surface,andirregular and coarse chromatin texture.CONCLUSION The serial optical tomograms ofCLSM can be used to create 3-D reconstruction ofcancer cell nuclei.Such 3-D impressions mightbe helpful or even essential in making anaccurate diagnosis.展开更多
基金the Applied Science Foundation of Jiangsu Province,China,No.BJ97071
文摘INTRODUCTIONThe treatment of human epithelial malignancies islimited by drug resistance and toxic and side effects,which results in the failure in the treatment ofmajority of advanced cancer victims.To seek for anew,and specific antineoplastic therapy willprovide hope for tumor treatment.Althoughdisordered intermediary metabolism in cancer cellshas been known for many years,much of the
文摘AIM To investigate the application of confocallaser scanning microscopy(CLSM)in tumorpathology and three-dimensional( 3-D )reconstruction by CLSM in pathologic specimensof hepatocellular carcinoma(HCC).METHODS The 30μm thick sections were cutfrom the paraffin-embedded tissues of HCC,hyperplasia and normal liver,stained with DNAfluorescent probe YOYO-1 iodide and examinedby CLSM to collect optical sections of nuclei and3-D images reconstructed.RESULTS HCC displayed chaotic arrangementof carcinoma cell nuclei,marked pleomorphism,indented and irregular nuclear surface,andirregular and coarse chromatin texture.CONCLUSION The serial optical tomograms ofCLSM can be used to create 3-D reconstruction ofcancer cell nuclei.Such 3-D impressions mightbe helpful or even essential in making anaccurate diagnosis.
