从河北省围场县某养殖场患病死亡的犊牛脏器样品中分离到1株优势菌株,对其进行常规形态学观察、生化鉴定、16S r DNA基因序列测定、动物致病性试验及药敏试验。结果显示,分离菌株的生化鉴定特性与粪肠球菌的生化鉴定特性相符;分离菌株16...从河北省围场县某养殖场患病死亡的犊牛脏器样品中分离到1株优势菌株,对其进行常规形态学观察、生化鉴定、16S r DNA基因序列测定、动物致病性试验及药敏试验。结果显示,分离菌株的生化鉴定特性与粪肠球菌的生化鉴定特性相符;分离菌株16Sr DNA基因序列与粪肠球菌的同源性为96. 2%;在系统发育进化树上分离菌株与粪肠球菌聚为一簇;分离菌株对小白鼠具有一定的致病性,并将其命名为EFC QHD-1;分离菌株EFC QHD-1对替米考星、复方新诺明、卡那霉素等耐药;对环丙沙星、氟苯尼考、阿莫西林等敏感。展开更多
In May 2016,an epizootic occured among cultured tongue soles caused mass deaths in a fish farm in Qinhuangdao,China.In order to find out the etiological agent,a bacterial strain was isolated from ascites and other tis...In May 2016,an epizootic occured among cultured tongue soles caused mass deaths in a fish farm in Qinhuangdao,China.In order to find out the etiological agent,a bacterial strain was isolated from ascites and other tissues of sick tongue sole aseptically collected.The isolate was identified as Photobacterium damselae subsp.damsela（PDD） by isolation culture,Gram staining,physiological identification,morohological observation,biochemical identification and 16S rDNA sequence analysis.The results showed that the isolate shared 99.6% homology with the reference strain in GenBank.The animal regression test displayed that the isolate had very strong pathogenicity to tongue sole.The LD（50） was 3.1 × 10~4 CFU/mL,and it showed pathogenicity to mammals.The antimicrobial susceptibility test showed the isolate was highly sensitive to nrofloxacin,Norfloxacin,Ciprofloxacin,Mequindox;moderately sensitive to Cefradine,Doxycycline;and insensitive to Gentamicin,Ceftriaxone,Tilmicosin,etc..展开更多
In order to study the mechanism of immunopotentiator, the quantity of T lymphocyte was observed. Total 240 1-day chikens were divided into 3 groups randomly： one contrast group and two groups being drunk immunopotent...In order to study the mechanism of immunopotentiator, the quantity of T lymphocyte was observed. Total 240 1-day chikens were divided into 3 groups randomly： one contrast group and two groups being drunk immunopotentiator according to the concentration 10 mL·L^-1 and 5 mL·L^-1 lasting for 48 d. The number of T lymphocyte in blood was measured by E-rosette when the chikens were at 12-, 24-, 36-, and 48-day. The results showed that the percent ofT lymphocyte in the trial group was obviously higher than that of the contrast, and the 10 mL·L^-1 group was higher than that of the 5 mL·L^-1 group; the quantity distribution of T lymphocyte in intestinal mucosa lymphoid tissue of 14-day, 21-day chiken was surveyed in the contrast group and the10 mL·L^-1 group with the method of routine histology-slices and ANAE, the results showed that the quantity of T lymphocyte in the 10 mL·L^-1 group was significantly higher than that of the contrast, which indicated that immunopotentiator increased markedly the quantity of T lymphocyte, and the 10 mL·L^-1 group was higher than that of the 5 mL·L^-1 group.展开更多
基金Supported by Incentive Subsidy Program of Hebei Department of Science and Technology(15926620H)Key Technology R&D Program of Qinhuangdao Science and Technology Bureau(201401A067)+1 种基金Prevention and Control of Major Bacterial Diseases in Industrial Farming Fishes(201602A341)Sereening and Preliminary Application of Protective Antigen of Two Important Marine Pathogens(2018HY007)
文摘In May 2016,an epizootic occured among cultured tongue soles caused mass deaths in a fish farm in Qinhuangdao,China.In order to find out the etiological agent,a bacterial strain was isolated from ascites and other tissues of sick tongue sole aseptically collected.The isolate was identified as Photobacterium damselae subsp.damsela（PDD） by isolation culture,Gram staining,physiological identification,morohological observation,biochemical identification and 16S rDNA sequence analysis.The results showed that the isolate shared 99.6% homology with the reference strain in GenBank.The animal regression test displayed that the isolate had very strong pathogenicity to tongue sole.The LD（50） was 3.1 × 10~4 CFU/mL,and it showed pathogenicity to mammals.The antimicrobial susceptibility test showed the isolate was highly sensitive to nrofloxacin,Norfloxacin,Ciprofloxacin,Mequindox;moderately sensitive to Cefradine,Doxycycline;and insensitive to Gentamicin,Ceftriaxone,Tilmicosin,etc..
文摘In order to study the mechanism of immunopotentiator, the quantity of T lymphocyte was observed. Total 240 1-day chikens were divided into 3 groups randomly： one contrast group and two groups being drunk immunopotentiator according to the concentration 10 mL·L^-1 and 5 mL·L^-1 lasting for 48 d. The number of T lymphocyte in blood was measured by E-rosette when the chikens were at 12-, 24-, 36-, and 48-day. The results showed that the percent ofT lymphocyte in the trial group was obviously higher than that of the contrast, and the 10 mL·L^-1 group was higher than that of the 5 mL·L^-1 group; the quantity distribution of T lymphocyte in intestinal mucosa lymphoid tissue of 14-day, 21-day chiken was surveyed in the contrast group and the10 mL·L^-1 group with the method of routine histology-slices and ANAE, the results showed that the quantity of T lymphocyte in the 10 mL·L^-1 group was significantly higher than that of the contrast, which indicated that immunopotentiator increased markedly the quantity of T lymphocyte, and the 10 mL·L^-1 group was higher than that of the 5 mL·L^-1 group.
