OsRRM, a Spen-like rice gene expressed specifically in the endosperm

我们使用了倡导者陷井技术识别米饭植物，说出 107 # ，在哪个 beta-glucuronidase (GUS ) 记者基因在内乳明确地被表示。T-DNA 的 Asingle 拷贝被插入到植物染色体，并且候选人基因 OsRRM 被插入识别。OsRRM 倡导者在米饭内乳明确地指导了 GUS 表示，类似于在 107 观察的 GUS 表示模式 # 。OsRRM 是在米饭的单个拷贝的基因并且编码与二个 RNA 识别主题和一根 Spen 帕拉木头和 ortholog C 终端领域包含 1005 氨基酸的残余的原子蛋白质。西方的污点分析证实那 theOsRRM 蛋白质明确地在米饭内乳被表示。在转基因的植物的 OsRRM 的宫外的表示导致了畸形，例如短身高，延迟的生长和低结果实率。我们的数据与 Spen 基因的报导函数一起，在在米饭内乳的房间开发的规定的 implicatedOsRRM。

Ultrasound biomicroscopic imaging demonstrate thinner ciliary body thickness in eyes with angle closure

·AIM:To compare the ciliary body thickness between eyes with primary angle closure(PAC)and primary angle-closure glaucoma(PACG)with the normal eyes,and to investigate the association between ciliary body thickness and ciliary processes situation.·METHODS:In this cross-sectional study,57 patients with PAC/PACG were matched to 57 normal subjects after propensity score matching(PSM)adjusting for age and gender.All subjects underwent conventional ocular examinations and ultrasound biomicroscopy(UBM)examination,among which the patients with PAC/PACG performed the examinations one month after laser peripheral iridotomy(LPI).Quantitative parameters were measured,which included ciliary body thickness at the position of 1 mm posterior to the scleral spur(CBT1),trabecular-ciliary process distance(TCPD)and trabecularciliary process angle(TCA).·RESULTS:Eyes with PAC/PACG presented significantly thinner CBT1,shorter TCPD and smaller TCA(P<0.001)than the normal eyes,both in comparison of the means of four quadrants and in comparisons of each quadrant.After removing images with peripheral anterior synechia(PAS),the same results were also found in comparisons between the two groups.Significant correlations were found between TCPD(R~2=0.537,P<0.001)and TCA(R~2=0.517,P<0.001)with CBT1.·CONCLUSION:Eyes with PAC/PACG have thinner ciliary body thickness and more anteriorly situated ciliary processes.Thinner ciliary body thickness is associated with anterior situation of the ciliary processes.

Diabetes patients with comorbidities had unfavorable outcomes following COVID-19:A retrospective study

BACKGROUND Previous studies have shown that diabetes mellitus is a common comorbidity of coronavirus disease 2019(COVID-19),but the effects of diabetes or anti-diabetic medication on the mortality of COVID-19 have not been well described.AIM To investigate the outcome of different statuses(with or without comorbidity)and anti-diabetic medication use before admission of diabetic after COVID-19.METHODS In this multicenter and retrospective study,we enrolled 1422 consecutive hospitalized patients from January 21,2020,to March 25,2020,at six hospitals in Hubei Province,China.The primary endpoint was in-hospital mortality.Epidemiological material,demographic information,clinical data,laboratory parameters,radiographic characteristics,treatment and outcome were extracted from electronic medical records using a standardized data collection form.Most of the laboratory data except fasting plasma glucose(FPG)were obtained in first hospitalization,and FPG was collected in the next day morning.Major clinical symptoms,vital signs at admission and comorbidities were collected.The treatment data included not only COVID-19 but also diabetes mellitus.The duration from the onset of symptoms to admission,illness severity,intensive care unit(ICU)admission,and length of hospital stay were also recorded.All data were checked by a team of sophisticated physicians.RESULTS Patients with diabetes were 10 years older than non-diabetic patients[(39-64)vs(56-70),P<0.001]and had a higher prevalence of comorbidities such as hypertension(55.5%vs 21.4%,P<0.001),coronary heart disease(CHD)(9.9%vs 3.5%,P<0.001),cerebrovascular disease(CVD)(3%vs 2.2%,P<0.001),and chronic kidney disease(CKD)(4.7%vs 1.5%,P=0.007).Mortality(13.6%vs 7.2%,P=0.003)was more prevalent among the diabetes group.Further analysis revealed that patients with diabetes who took acarbose had a lower mortality rate(2.2%vs 26.1,P<0.01).Multivariable Cox regression showed that male sex[hazard ratio(HR)2.59(1.68-3.99),P<0.001],hypertension[HR 1.75(1.18-2.60),P=0.006),CKD[HR 4.55(2.52-8.20),P<0.001],CVD[HR 2.35(1.27-4.33),P=0.006],and age were risk factors for the COVID-19 mortality.Higher HRs were noted in those aged≥65(HR 11.8[4.6-30.2],P<0.001)vs 50-64 years(HR 5.86[2.27-15.12],P<0.001).The survival curve revealed that,compared with the diabetes only group,the mortality was increased in the diabetes with comorbidities group(P=0.009)but was not significantly different from the noncomorbidity group(P=0.59).CONCLUSION Patients with diabetes had worse outcomes when suffering from COVID-19;however,the outcome was not associated with diabetes itself but with comorbidities.Furthermore,acarbose could reduce the mortality in diabetic.

Regulating effects of mangiferin on oxidative stress response and mitochondrial pathway apoptosis in spinal cord injury model

Objective:To study the regulating effects of mangiferin on oxidative stress response and mitochondrial pathway apoptosis in spinal cord injury model.Methods: SD rats were selected as the experimental animals and divided into the control group that underwent sham operation, the injury group that were made into spinal cord injury models and the mangiferin group that were made into spinal cord injury models and given mangiferin intervention. 30 d after the intervention, spinal cord tissue was collected, radioimmunoprecipitation kit was used to determine the contents of oxidative stress indexes ROS, H2O2, SOD, GSH-Px and CAT, and enzyme-linked immunosorbent assay kit was used to determine the contents of apoptosis indexes SKIP, ASK1, Fas, tBid, APAF-1 and Caspase-3.Results:ROS, H2O2, SKIP, ASK1, Fas, tBid, APAF-1 and caspase-3 contents in spinal cord tissue of injury group were significantly higher than those of control group whereas SOD, GSH-Px and CAT contents were significantly lower than those of control group;ROS, H2O2, SKIP, ASK1, Fas, tBid, APAF-1 and caspase-3 contents in spinal cord tissue of mangiferin group were significantly lower than those of injury group whereas SOD, GSH-Px and CAT contents were significantly higher than those of injury group.Conclusion:Mangiferin has inhibitory effect on oxidative stress response and mitochondrial pathway apoptosis in spinal cord injury model.

Establishing a Gene Trap System Mediated by T-DNA(GUS) in Rice

Two plasmids, p13GUS and p13GUS2, were constructed to create a gene trap system containing the promotsriess β-glucuronidase （GUS） reporter gene in the T-DNA region. Transformation of these two plasmids into the rice variety Zhonghua 11 （Oryza sativa ssp. japonica cv.）, mediated by Agrobacterium tumefaciens, resulted in 942 independent transgenic lines. Histochemical GUS assays revealed that 31 To plants had various patterns of the reporter gene expression, including expression in only one tissue, and simultaneously in two or more tissues. Hygromycin-resistsnt （hygr） homozygotes were screened and the copy number of the T-DNA inserts was determined in the GUS-positive transgenic plants. The flanking sequences of the T-DNA were isolated by inverse-polymerase chain reaction and the insert positions on the rice genome of T-DNA were determined by a basic local alignment search tool in the GUS-positive transgenic plants transformed with plasmid p13GUS. Moreover, calli induced from the seeds of the T1 generation of 911 GUS-negative transgenic lines were subjected to stress and hormone treatments. Histochemical GUS assays were carried out on the calli before and after treatment. The results revealed that calli from 21 lines displayed differential GUS expression after treatment. All of these data demonstrated that this trap system is suitable for identifying rice genes, including those that are sensitive to induction.

针对I/O密集型应用的新型非易失存储系统（英文）

新型存储技术——如相变存储器(phase change memory, PCM——的兴起,为I/O密集型应用的高性能存储提供了新机遇。然而传统软件栈和硬件架构需要重构优化以提升I/O访问效率。此外,存储内计算技术已经成为当前研究热点,能有效减少I/O请求数量。提出一种基于相变存储器的新型存储系统,包括支持存储内计算的文件系统(in-storage processing enabled file system, ISPFS)和可配置并行计算的存储内计算引擎。一方面,ISPFS充分利用新型非易失存储器(non-volatile memory, NVM)的特性,减少软件冗余和数据拷贝,提供低延迟、高性能的随机访问。另一方面,ISPFS通过命令文件将ISP指令发送给存储内计算引擎来处理I/O密集型任务。实验证明,ISPFS的吞吐量是EXT4文件系统的2–10倍。对于I/O密集型应用,该存储内计算方案比传统软件方案减少97%的I/O请求,效率提升19倍。

Involvement of unusual noncovalent interactions in the self-assembly of cucurbit[6]uril with [CdCl_4]^(2-) anions

The [CdCl_4]^（2-） anion as a structure inducer has proved to be useful in the construction of cucurbit[n]urilmetal coordination architectures and materials. In order to better understand the role and influence of the structure inducer in Q[n] systems, we report herein the self-assembly of Q[6] solely in the presence of[CdCl_4]^（2-）anions and in the presence of both a linear cationic organic guest and [CdCl_4]^（2-）anions. X-ray diffraction analysis revealed that 1D Q[6] porous channels were formed by the noncovalent interactions between Q[6] and [CdCl_4]^（2-）anions, but the ＇＇honeycomb effect＇＇ was not observed in the present study.However, it seems that the ＇＇honeycomb effect＇＇ and the self-assembly of Q[6] with [CdCl_4]^（2-）anions can be significantly modified and switched in the presence of a linear cationic dibutylamine guest through some unusual noncovalent interactions.