Predicting the Activity of Oral Lichen Planus with Glycolysis-related Molecules:A Scikit-learn-based Function

Objective Oral lichen planus(OLP)is one of the most common oral mucosa diseases,and is mainly mediated by T lymphocytes.The metabolic reprogramming of activated T cells has been shown to transform from oxidative phosphorylation to aerobic glycolysis.The present study investigated the serum levels of glycolysis-related molecules(lactate dehydrogenase,LDH;pyruvic acid,PA;lactic acid,LAC)in OLP,and the correlation with OLP activity was assessed using the reticular,atrophic and erosive lesion(RAE)scoring system.Methods Univariate and multivariate linear regression functions based on scikit-learn were designed to predict the RAE scores in OLP patients,and the performance of these two machine learning functions was compared.Results The results revealed that the serum levels of PA and LAC were upregulated in erosive OLP(EOLP)patients,when compared to healthy volunteers.Furthermore,the LDH and LAC levels were significantly higher in the EOLP group than in the nonerosive OLP(NEOLP)group.All glycolysis-related molecules were positively correlated to the RAE scores.Among these,LAC had a strong correlation.The univariate function that involved the LAC level and the multivariate function that involved all glycolysis-related molecules presented comparable prediction accuracy and stability,but the latter was more time-consuming.Conclusion It can be concluded that the serum LAC level can be a user-friendly biomarker to monitor the OLP activity,based on the univariate function developed in the present study.The intervention of the glycolytic pathway may provide a potential therapeutic strategy.

Protective effect of exogenous IGF-I on the intestinal mucosal barrier in rats with severe acute pancreatitis

BACKGROUND:Severe acute pancreatitis(SAP) can result in intestinal mucosal barrier(IMB)dysfunction.This study was undertaken to demonstrate the effect of IGF-I on the intestinal mucosal barrier in rats with SAP and its possible mechanisms.METHODS:Seventy-two male Wistar rats were randomly divided into three groups:a sham operation(SO group,n=24),a SAP group not treated with IGF-I(SAP group,n=24),and a SAP group treated with IGF-I(IGF-I group,n=24).SAP was induced in the rats by injecting 5.0%sodium taurocholate into the biliary-pancreatic duct.The SO rats were given an infusion of normal saline instead.The rats in the IGF-I group underwent the SAP procedure and were given a subcutaneous injection of IGF-I at 30 minutes before the operation and at 3 hours after the operation.Eight rats in each group were sacrificed at 6,12 and 24 hours after operation.Apoptosis of mucosal cells in the small intestine was determined by TUNEL.The levels of endotoxin and DAO and serum amylase were also measured.Pathologic changes in the small intestine were monitored.Changes of bax and bcl-2 mRNA expression in the small intestine were determined by reverse transcription polymerase chain reaction(RT-PCR).RESULTS:The levels of serum amylase were lower in the IGF-I group than in the SAP group at all three time points(P<0.05).The levels of endotoxin in the IGF-I group were higher than those in the SAP group at 6 hours,but lower in the IGF-I group than in the SAP group at 12 and 24 hours(P<0.05).The levels of diamine oxidase were higher in the IGF-I group at 6 hours but lower than those in the SAP group at 12 and 24 hours.The pathological score of the small intestine was lower in the IGF-I group than in the SAP group,and the difference was statistically significant at 12 and 24 hours.The pathologic changes observed under electron microscopy were better in the IGF-I group than those in the SAP group.The apoptosis index of intestinal epithelial cells was significantly decreased in the IGF-I group compared with the SAP group.Compared with the SO group,the mRNA expression levels of bax were increased at each time point in the SAP group,and were significantly decreased in the IGF-I group as compared with the SAP group at each time point(P<.05).The expression levels of bcl-2 were weak and not different between the SO group and the SAP group(P>0.05).They were significantly increased in the IGF-I group versus the SO and SAP groups(P<0.05).The ratio of bax and bcl-2 mRNA expression levels at each time point in the SAP group were significantly higher than those in the SO group,but they were obviously decreased in the IGF-I group.CONCLUSIONS:Exogenous IGF-I seems to protect mucosal cells in the small intestine against SAP-induced apoptosis and could alleviate SAP-induced injury of the intestinal mucosa.The underlying mechanisms include enhanced mRNA expression of bcl-2 and inhibition of bax mRNA expression.

In situ observation of surface structures of cardiovascular endothelial cells with atomic force microscope

目的将与原子力量显微镜(AFM ) 在 situ 观察心血管的 endothelial 房间的表面结构。方法新鲜主动脉和大动脉的阀门从 10 只健康雄的新西兰白兔子被把。在在 1% 甲醛修理了以前，新鲜纸巾在缓冲区磷酸盐答案被洗。在一般显微镜下面，固定主动脉或阀门在已经在玻璃幻灯片上被粘住的双方面棍子磁带上被散布。主动脉的 intima 或阀门的主动脉方面向上边。然后，标本在在保温装置摄氏的 37 度下面被弄干并且与纯水被洗。在再弄干以后，标本在站台 ofNanoScope llla AFM 上被装载并且在以 0.5 HZ 的扫描速度拍模式被扫描。表面在固定并且弄干的织物上 endothelial 房间组织的结果能在有 AFM 的 situ 清楚地是 obsserved。大动脉的 endothclial 房间稀疏地大，分叉并且安排并且而主动脉阀门表面上的 endothelial 房间是小的，到血流动的方向的平行少些强烈地分叉并且安排并且对血的方向垂直流动。当扫描范围被缩小的、小粒的超微结构能在 endothelial 房间的表面上被观察时，当扫描范围进一步被缩小，裂缝和卷绕旋转能从大动脉的 endothelial 房间在小粒的表面上被看见。中心洞和包围胀大的像火山的结构能从大动脉的阀门的 endothelial 房间在小粒的表面上被看见。结论与 FM 提供的 AFM 和词法信息在 situ 观察心血管的 endothelial 房间的表面超微结构是可行的可能具有在未来的临床的价值组织病理学说的诊断。

Effectiveness of Hemorrhagic Fever with Renal Syndrome Bivalent Vaccine in China:A Metaanalysis

Objective To evaluate the effectiveness of Hemorrhagic Fever with Renal Syndrome(HFRS) bivalent vaccine in field.Methods The articles on effectiveness of HFRS bivalent vaccine were retrieved from Vip Database,China Journal Full-text Database,Wanfang database,China Hospital Knowledge Database,Chinese Biomedical Literature Database,MEDLINE and ProQuest.Two persons extracted data and evaluated the quality of data together for meta-analysis.Results Seven articles were included into the analysis,and two articles were random clinical trials,five were quasi-trials.Meta-analysis showed that the overall effectiveness of HFRS bivalent vaccine was 85%(95%confidence interval:53%-95%) within one year after vaccination,and the effectiveness in two-dose recipients and three-dose recipients was 87%(95%confidence interval:54%-96%) and 60%,respectively;it was 96%(95%confidence interval:78%-99%) within 2-5 years after vaccination,and the effectiveness in two-dose recipients and three-dose recipients was 95%(95%confidence interval:19%-100%) and 96%(95%confidence interval:70%-99%),respectively;it was 100%(only one article included) within 6-9.6 years after vaccination.The effectiveness in two-dose recipients was 88%(95%confidence interval:62%-96%) during 1-2.5 years after vaccination,and that in three-dose recipients was 94%(95%confidence interval:70%-99%) during 1-5 years after vaccination.Conclusions HFRS bivalent vaccine is effective in field,and there is no difference between two-dose recipients and three-dose recipients.We should do some further studies on the long-term effectiveness of HFRS bivalent vaccine and on the age of vaccine reception.

Recent progresses on alloy-based anodes for potassium-ion batteries

Potassium-ion batteries(KIBs)are one of the most promising large-scale electric energy storage systems due to the high abundance and low redox potential of K.As the key component,anode determines their energy density and safety.Alloy-based anodes,such as P,Sn,Sb,and Bi,have attracted extensive attention due to their abundant resources,suitable working potentials,and large theoretical capacities.However,the dramatic volume variation upon(de)potassiation results in pulverization of particles and their detaching from the current collector accompanied with performance decay.Various strategies,including designing micro-/nanostructures,introducing carbon substrates,and optimizing electrode/electrolyte interface,have been demonstrated to effectively alleviate these issues.Herein,we summarize the recent research progresses on alloy-based materials in KIBs.The synthesis methods,electrochemical performance,reaction mechanisms,and structure-activity relationships of these materials are considered,and challenges and perspectives are provided.This review provides new insight into designing of high-activity electrode materials for KIBs and beyond.