Effect of levocarnitine on TIMP-1,ICAM-1 expression of rats with coronary heart disease and its myocardial protection effect

Objective:To study the effect of levocarnitine(L-CN) on tissue inhibitor of metalloproteinase-1(TIMP-1) and intercellular adhesion molecule-1(ICAM-1) expression of rats with coronary heart disease and evaluate the protective effect of L-CN on myocardial cells.Methods:Highfat diet feeding and intraperitoneal injection of pituitrin was performed on rats in model group and CHD Model of rats was built.Rats with successful model-building were selected and divided into L-CN group and Ctrl group randomly.Rats in L-CN group were given L-CN treatment,with intraperitoneal injection of 200 mg·kg^(-1)?d^(-1) and successive administration for 3 d.Rats in Ctrl group were given equal volumes of normal saline.Blood was collected from carotid artery at different time and expression quantity of creatine kinase-MB(CK-MB) and Troponin Ⅰ(Tn Ⅰ)in serum was detected.Rats in each group were put to death and were separated to obtain the myocardial tissue.Real-time PCR and Western Blotting hybridization were performed to detect the TIMP-1.ICAM-1 expression in myocardial tissue in each group.Statistical analysis was employed to explore the expression changes of TIMP-1 and ICAM-1.and ELISA test was used lo analyse the expression changes of myocardial necrosis marker- CK-MB and Tn I to learn the effect of L-CN and its myocardial protective effect.Results:The total cholesterol,triglyceride and blood glucose levels of rats in model group were significantly higher than that in control group,which indicated that due to high-fat diet feeding,blood lipid of rats in model group was obviously higher than that in control group.In myocardial tissue of rats in model group,TIMP-I level significantly reduced and ICAM-1 level significantly increased(P<0.0l).In model group,after L-CN treatment.TTMP-I level had double increase,while ICAM-1 level had 43%of decrease in L-CN group compared with Ctrl group.After L-CN intervention treatment.CK-MB and Tn Ⅰ content in L-CN group relatively reduced compared with Ctrl group.The difference among groups was obvious(P<0.01).Condusions:L-CN could increase the TTMP-I expression level and inhibit the ICAM-1 expression level.L-CN has a certain myocardial protective

Efficient vulnerability detection based on an optimized rule-checking static analysis technique

Static analysis is an efficient approach for software assurance. It is indicated that its most effective usage is to perform analysis in an interactive way through the software development process, which has a high performance requirement. This paper concentrates on rule-based static analysis tools and proposes an optimized rule-checking algorithm. Our technique improves the performance of static analysis tools by filtering vulnerability rules in terms of characteristic objects before checking source files. Since a source file always contains vulnerabilities of a small part of rules rather than all, our approach may achieve better performance. To investigate our technique's feasibility and effectiveness, we implemented it in an open source static analysis tool called PMD and used it to conduct experiments. Experimental results show that our approach can obtain an average performance promotion of 28.7% compared with the original PMD. While our approach is effective and precise in detecting vulnerabilities, there is no side effect.

一种用于程序约束挖掘的过采样方法（英文）

自动协议挖掘是获取精确而完备的API使用协议的有效方法。然而,与其它数据挖掘应用类似,自动协议挖掘方法需要足够多训练数据(即对象使用场景)作为输入。虽然通过增加程序的规模可提取更多数量的对象使用场景,但这会导致程序分析运行时开销较大。本文针对面向对象程序提出一种基于继承关系的对象使用场景过采样方法。给定一个面向对象程序p,一般情况下,执行p所能获得的对象使用场景数不超过运行时实例化的对象数。而本文方法可获得多达上述n倍的对象使用场景,其中n为程序p中一般对象使用场景的平均父类数。为了验证效果,在前期API使用协议动态挖掘原型工具ISpecMiner中集成上述方法并开展实验研究。实验采用扩展后的ISpec Miner从多个实际的程序中挖掘API使用协议。结果显示,采用本文方法获得的对象使用场景数是一般化方法的1.95倍。不仅如此,对比实验结果表明本文方法有利于挖掘更加精确而完备的API使用协议。特别值得关注的是,本文方法适用于无法实例化的类并挖掘出其API使用协议。这类API使用协议对于验证软件架构、程序说明和理解具有重要意义。虽然本文方法会增加一定的运行开销,但其仍在可接受范围内。