腹膜后去分化脂肪肉瘤的CT和MRI表现

目的:探讨腹膜后去分化脂肪肉瘤(dedifferentiated liposarcoma,DDL)的CT及MRI表现,旨在提高对腹膜后DDL的认识和术前诊断的准确率。方法:回顾性分析2012年1月至2022年6月天津医科大学肿瘤医院经病理证实的25例腹膜后DDL患者的临床及影像学特征。结果:25例患者中单发19例、多发6例,10例呈类圆(椭圆)形、15例呈不规则形,患者中病变多数边界不清,15例侵犯周围组织器官。25例患者中15例可见增粗扭曲血管影、7例可见钙化或骨化、仅3例可见囊变坏死。CT或MRI增强扫描呈“慢进慢出”的向心性、渐进性持续强化特点。根据其CT和MRI表现分为两型:Ⅰ型(软组织肿块型)肿瘤为软组织成分肿块,内不见脂肪成分(14例);Ⅱ型(含脂型)肿瘤内同时见软组织成分及脂肪成分,两者大多界限清楚,较少呈镶嵌状,异常脂肪区域内可见索条状纤维间隔,其中以瘤内脂肪成分<50%为Ⅱa型(10例),瘤内脂肪成分≥50%为Ⅱb型(1例)。结论:结合影像学分型,综合分析腹膜后DDL的CT和MRI影像学特点,对其术前定性诊断有重要价值。

Capturing the hierarchically assorted modules of protein–protein interactions in the organized nucleome

Nuclear proteins are major constituents and key regulators of nucleome topological organization and manipulators of nuclear events.To decipher the global connectivity of nuclear proteins and the hierarchically organized modules of their interactions,we conducted two rounds of cross-linking mass spectrometry(XL-MS)analysis,one of which followed a quantitative double chemical cross-linking mass spectrometry(in vivo qXL-MS)workflow,and identified 24,140 unique crosslinks in total from the nuclei of soybean seedlings.This in vivo quantitative interactomics enabled the identification of 5340 crosslinks that can be converted into 1297 nuclear protein–protein interactions(PPIs),1220(94%)of which were non-confirmative(or novel)nuclear PPIs compared with those in repositories.There were 250 and 26 novel interactors of histones and the nucleolar box C/D small nucleolar ribonucleoprotein complex,respectively.Modulomic analysis of orthologous Arabidopsis PPIs produced 27 and 24 master nuclear PPI modules(NPIMs)that contain the condensate-forming protein(s)and the intrinsically disordered region–containing proteins,respectively.These NPIMs successfully captured previously reported nuclear protein complexes and nuclear bodies in the nucleus.Surprisingly,these NPIMs were hierarchically assorted into four higher-order communities in a nucleomic graph,including genome and nucleolus communities.This combinatorial pipeline of 4C quantitative interactomics and PPI network modularization revealed 17 ethylene-specific module variants that participate in a broad range of nuclear events.The pipeline was able to capture both nuclear protein complexes and nuclear bodies,construct the topological architectures of PPI modules and module variants in the nucleome,and probably map the protein compositions of biomolecular condensates.

Bone analysis using an aggregation-induced emission-active iridium complex

Fluorescent analysis of bone provides valuable insights into bone structures.However,conventional dyes suffer from low specificity on bone tissue,small stokes shift,short fluorescent lifetime,and aggregation-caused quenching effect,which result in low efficacy and artifacts.In this work,we design an aggregation-induced emission(AIE)-active iridium(III)complex(Ir-BP2)as a highly selective,convenient,nondestructiveness,and dual-mode staining agent for bone analysis.Ir-BP2 containing phosphonate groups selectively binds to hydroxyapatites,the main component of bone matrix,and exhibits turn-on AIE phosphorescence with prolonged lifetime.Ir-BP2 exhibits promising biosafety and offers higher accuracy in staining calcium deposits than conventional Alizarin Red S staining assay when it is employed in real-time monitoring of osteogenesis differentiation process.A ready-to-use staining spray of Ir-BP2 is fabricated.By using fluorescent imaging and lifetime imaging,Ir-BP2 staining provides valuable insights into bone microstructure analysis,microdamage diagnosis,and bone growth state identification.Further,Ir-BP2 is successfully applied on a human spine vertebra for diagnosing bone invasiveness of eosinophilic granuloma,validating its clinical practice.This work presents a powerful tool in bone analysis and will lead to new approaches for the diagnosis and treatment of bone-related diseases.

Achieving Fine-Grained Microstructure and Superior Mechanical Property in a Plain Low-Carbon Steel Using Heavy Cold Rolling Combined with Short-Time Heat Treatment

A new thermomechanical process consisting of heavy cold rolling(HCR)and short-time heat treatment(STH)is developed to fabricate fine-grained martensite microstructure in a low-cost plain low-carbon steel.To achieve the optimal mechanical properties after STH,three different ferrite-pearlite(F-P)dual-phase microstructures are prepared via hot rolling(HR),HR and austenitizing,and HR and HCR.The microstructure evolution and the comprehensive mechanical properties of the alloy during STH are then investigated.We find that the volume fractions of transformed martensite after STH increase with decreasing grain sizes of the pre-STH F-P dual phases.The rapid heating and short-time holding of STH promote grain nucleation and inhibit grain growth,resulting in microstructure refinement.The formation of martensites with different morphologies and different carbon concentrations in the HR and HCR+STH alloy is identified,owing to the inhomogeneous carbon distribution by STH.Tensile experiments demonstrate that STH greatly improves the comprehension mechanical properties of the alloy.Excellent mechanical properties,with a yield strength of 1224 MPa,a tensile strength of 1583 MPa,a uniform elongation of 4.0%and a total elongation of 7.3%are achieved in the HR and HCR+STH alloy.These excellent mechanical properties are principally attributed to the microstructure refinement and martensite formation induced by STH,with a yield strength improvement of 134%and a tensile strength improvement of 150%relative to the HR alloy.

Quantitative and Functional Phosphoproteomic Analysis Reveals that Ethylene Regulates Water Transport via the C-Terminal Phosphorylation of Aquaporin PIP2;1 in Arabidopsis

Ethylene participates in the regulation of numerous cellular events and biological processes, including wa- ter loss, during leaf and flower petal wilting. The diverse ethylene responses may be regulated via dynamic interplays between protein phosphorylation/dephosphorylation and ubiquitin/26S proteasome-mediated protein degradation and protease cleavage. To address how ethylene alters protein phosphorylation through multi-furcated signaling pathways, we performed a lSN stable isotope labelling-based, differential, and quantitative phosphoproteomics study on air- and ethylene-treated ethylene-insensitive Arabidopsis double loss-of-function mutant ein3-1/eill-1. Among 535 non-redundant phosphopeptides identified, two and four phosphopeptides were up- and downregulated by ethylene, respectively. Ethylene- regulated phosphorylation of aquaporin PIP2;1 is positively correlated with the water flux rate and water loss in leaf. Genetic studies in combination with quantitative proteomics, immunoblot analysis, protoplast swelling/shrinking experiments, and leaf water loss assays on the transgenic plants expressing both the wild-type and S280A/S283A-mutated PIP2;1 in the both Col-O and ein3eill genetic backgrounds suggest that ethylene increases water transport rate in Arabidopsis cells by enhancing S280/S283 phosphorylation at the C terminus of PIP2;1. Unknown kinase and/or phosphatase activities may participate in the initial up- regulation independent of the cellular functions of EIN3/EIL1. This finding contributes to our understanding of ethylene-regulated leaf wilting that is commonly observed during post-harvest storage of plant organs.

Research Progress of Metal-Based Shielding Materials for Neutron and Gamma Rays

The radiation generated by nuclear reaction is harmful to human body and equipment,thus the radiation shielding materials that employ the shielding ability from neutron and gamma rays are the best candidates according to application situations and radiation sources.In this paper,the researches of metal-based neutron and gamma rays or multiple purpose shielding materials are systematically summarized,and the respective and principal problems of these materials with respect to shielding effectiveness and other performances,such as corrosion,mechanical properties,manufacture,etc.,are discussed.Finally,the prospect of shielding materials is outlined,which suggests that the development of highly efficient and multiply functional radiation shielding materials with good environmental compatibility is one of the future development trends.

High strength-ductility nano-structured high manganese steel produced by cryogenic asymmetry-rolling

A bulk nanostructured twinning-induced plasticity （TWIP） steel with high ductility and high strength was fabricated by cryogenic asymmetry-rolling （cryo-ASR） and subsequent recovery treatment. It was found that the cryo-ASRed TWIP steels exhibit simultaneous improvements in the ductility, strength and work hardening. Typical microstructures of the cryo-ASR TWIP steel were characterized by shear bands and intensive mechanical nano-sized twins induced by cryogenic deformation. These mechanical nano-scale twins remain thermally stable during the subsequent recovery treatment. It is believed that the ductility enhancement and high work-hardening ability for the cryo-ASR TWIP steels should be mainly attributed to the high-densitv pre-existing nano-scale twins.

Quantitative Analysis of Cytokinins in Plants by High Performance Liquid Chromatography:Electronspray Ionization Ion Trap Mass Spectrometry

The present paper introduces a highly sensitive and selective method for simultaneous quantification of 12 cytokinins （free form and their conjugates）. The method includes a protocol of extraction with methanol/water/formic acid （15/4/1, v/v/v） to the micro-scale samples, pre-purification with solid phase extraction （SPE） cartridges of the extracts, separation with a high performance liquid chromatography （HPLC） and detection by an electrospray ionization ion trap mass spectrometry （ESl-lon trap-MS） system in a consecutive ion monitoring （CRM） mode at the three stage fragmentation of mass spectrometry （MS3）. The lowest detection level of the cytokinins of the method reaches 0.1-2.0 pg with a very wide range of linear regression from 1-512 pg, at the coefficient factors of 0.98-0.99. The feasibility of this method has been proven in the application of the method to the analysis of the trace-amount contents of cytokinins in the micro-scale samples of various types of plant materials, such as aerial parts of rice and poplar leaves etc. 12 endogenous cytokinins had been identified and quantified in the plant tissues, with an acceptable relatively higher recovery rate from 40% to 70%.

Deep learning based water leakage detection for shield tunnel lining

Shield tunnel lining is prone to water leakage,which may further bring about corrosion and structural damage to the walls,potentially leading to dangerous accidents.To avoid tedious and inefficient manual inspection,many projects use artificial intelligence(Al)to detect cracks and water leakage.A novel method for water leakage inspection in shield tunnel lining that utilizes deep learning is introduced in this paper.Our proposal includes a ConvNeXt-S backbone,deconvolutional-feature pyramid network(D-FPN),spatial attention module(SPAM).and a detection head.It can extract representative features of leaking areas to aid inspection processes.To further improve the model's robustness,we innovatively use an inversed low-light enhancement method to convert normally illuminated images to low light ones and introduce them into the training samples.Validation experiments are performed,achieving the average precision(AP)score of 56.8%,which outperforms previous work by a margin of 5.7%.Visualization illustrations also support our method's practical effectiveness.