DearEditors,Rett Syndrome(RTT)is a severe neurodevelopmental disorder characterized by neural dysfunctions and a reduced lifespan,mainly in female patients,involving loss-of-function mutations in the methyl-CpG bindin...DearEditors,Rett Syndrome(RTT)is a severe neurodevelopmental disorder characterized by neural dysfunctions and a reduced lifespan,mainly in female patients,involving loss-of-function mutations in the methyl-CpG binding protein 2(MECP2)gene[1-4].展开更多
Dear Editor,Coronavirus disease 2019(COVID-19)is a highly infectious respiratory disease that continues to pose a serious global public health emergency.The disease shows a high infection rate,long incubation period,a...Dear Editor,Coronavirus disease 2019(COVID-19)is a highly infectious respiratory disease that continues to pose a serious global public health emergency.The disease shows a high infection rate,long incubation period,and rapidly emerging variants,which have led to its rapid spread worldwide(Krammer 2020).Many vaccines have been developed for the control of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),the virus responsible for COVID-19,including vaccines based on messenger RNA(mRNA)(Polack et al.2020),viral vectors(Zhu et al.2020),recombinant proteins(Yang et al.2020),and inactivated SARS-CoV-2(Zhang et al.2021).展开更多
Background:Amyotrophic lateral sclerosis(ALS)is a late onset neurodegenerative disease with fast progression.ALS has heavy genetic components in which a series of genetic mutations have been identified.In 2013,Mutatio...Background:Amyotrophic lateral sclerosis(ALS)is a late onset neurodegenerative disease with fast progression.ALS has heavy genetic components in which a series of genetic mutations have been identified.In 2013,Mutations of the CREST gene(also known as SS18L1),which functions as a calcium-regulated transcriptional activator,were found in sporadic ALS patients.However,the pathogenic causality and mechanisms of ALS-associated mutations of CREST remain to be determined.Methods:In this study,we constructed CREST knockout and Q394X knock-in mice with CRISPR/Cas9 system.Using biochemical and imaging tools,we illustrated core pathological phenotypes in CREST mutant mice and claimed the possible pathogenic mechanisms.Furthermore,we also observed locomotion defects in CREST mutant mice with behavioural tests.Results:We demonstrate that ALS-related CREST-Q388X mutation exhibits loss-of-function effects.Importantly,the microglial activation was prevalent in CREST haploinsufficiency mice and Q394X mice mimicking the human CREST Q388X mutation.Furthermore,we showed that both CREST haploinsufficiency and Q394X mice displayed deficits in motor coordination.Finally,we identified the critical role of CREST-BRG1 complex in repressing the expression of immune-related cytokines including Ccl2 and Cxcl10 in neurons,via histone deacetylation,providing the molecular mechanisms underlying inflammatory responses within mice lack of CREST.Conclusion:Our findings indicate that elevated inflammatory responses in a subset of ALS may be caused by neuron-derived factors,suggesting potential therapeutic methods through inflammation pathways.展开更多
Dilated cardiomyopathy(DCM) is a primary myocardial disease of unknown cause that is characterized by ventricular enlargement and ventricular systolic dysfunction(Reichart et al., 2019). DCM exhibits obvious heterogen...Dilated cardiomyopathy(DCM) is a primary myocardial disease of unknown cause that is characterized by ventricular enlargement and ventricular systolic dysfunction(Reichart et al., 2019). DCM exhibits obvious heterogeneity, and its outcomes extend from arrhythmia to heart failure. Early arrhythmia is a common condition that may progressively become aggravated, and death can occur at any stage of the disease. The most serious complications are heart failure and sudden death(Mc Nally et al., 2013).展开更多
基金supported by grants from the Youth Innovation Promotion Association Chinese Academy of Sciences(2022269)the Youth Science Fund of State Key Laboratory of Neuroscience(SKLN-2022B006)+5 种基金the National Natural Science Foundation of China(82001211,81941015,82021001)the CPSF-CAS Joint Foundation for Excellent Postdoctoral Fellows(2017LH036)the China Postdoctoral Science Foundation(2017M620173)the Strategic Priority Research Program of the Chinese Academy of Sciences(XDBS01060200)the Program of Shanghai Academic Research Leader,the Open Large Infrastructure Research of Chinese Academy of Sciences,the Shanghai Municipal Science and Technology Major Project(2018SHZDZX05)the Guangdong Key Scientific and Technological Project(2018B030335001).
文摘DearEditors,Rett Syndrome(RTT)is a severe neurodevelopmental disorder characterized by neural dysfunctions and a reduced lifespan,mainly in female patients,involving loss-of-function mutations in the methyl-CpG binding protein 2(MECP2)gene[1-4].
基金supported by Strategic Priority Research Program of the Chinese Academy of Sciences(XDB39000000 to T.X.)National Natural Science Foundation of China(81925009 to T.X.,81790644 to T.X.,81900855 to M.Z.,82000941 to D.T.)+3 种基金Jack Ma Foundation(2019-nCoV)CAS Project for Young Scientists in Basic Research(YSBR-013)Fundamental Research Funds for the Central Universities(WK5290000001 to Y.C.,WK5290000002 to Y.Y.,WK2090050048 to M.Z.,WK2070000174 to M.Z.)supported by the Anhui Provincial Natural Science Foundation(1808085MH289 to M.Z.).Joint Laboratory of Innovation in Life Sciences from the University of Science and Technology of China(USTC)and Changchun Zhuoyi Biological Co.Ltd.
文摘Dear Editor,Coronavirus disease 2019(COVID-19)is a highly infectious respiratory disease that continues to pose a serious global public health emergency.The disease shows a high infection rate,long incubation period,and rapidly emerging variants,which have led to its rapid spread worldwide(Krammer 2020).Many vaccines have been developed for the control of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),the virus responsible for COVID-19,including vaccines based on messenger RNA(mRNA)(Polack et al.2020),viral vectors(Zhu et al.2020),recombinant proteins(Yang et al.2020),and inactivated SARS-CoV-2(Zhang et al.2021).
基金This work was supported by NSFC Grants(#31625013,#91732302)Shanghai Brain-Intelligence Project from STCSM(16JC1420501).
文摘Background:Amyotrophic lateral sclerosis(ALS)is a late onset neurodegenerative disease with fast progression.ALS has heavy genetic components in which a series of genetic mutations have been identified.In 2013,Mutations of the CREST gene(also known as SS18L1),which functions as a calcium-regulated transcriptional activator,were found in sporadic ALS patients.However,the pathogenic causality and mechanisms of ALS-associated mutations of CREST remain to be determined.Methods:In this study,we constructed CREST knockout and Q394X knock-in mice with CRISPR/Cas9 system.Using biochemical and imaging tools,we illustrated core pathological phenotypes in CREST mutant mice and claimed the possible pathogenic mechanisms.Furthermore,we also observed locomotion defects in CREST mutant mice with behavioural tests.Results:We demonstrate that ALS-related CREST-Q388X mutation exhibits loss-of-function effects.Importantly,the microglial activation was prevalent in CREST haploinsufficiency mice and Q394X mice mimicking the human CREST Q388X mutation.Furthermore,we showed that both CREST haploinsufficiency and Q394X mice displayed deficits in motor coordination.Finally,we identified the critical role of CREST-BRG1 complex in repressing the expression of immune-related cytokines including Ccl2 and Cxcl10 in neurons,via histone deacetylation,providing the molecular mechanisms underlying inflammatory responses within mice lack of CREST.Conclusion:Our findings indicate that elevated inflammatory responses in a subset of ALS may be caused by neuron-derived factors,suggesting potential therapeutic methods through inflammation pathways.
基金supported by NSFC Grants(#82001836)the Shanghai Science and Technology Committee Foundation(#17411954400)the Shanghai Brain-Intelligence Project from STCSM(16JC1420501)。
文摘Dilated cardiomyopathy(DCM) is a primary myocardial disease of unknown cause that is characterized by ventricular enlargement and ventricular systolic dysfunction(Reichart et al., 2019). DCM exhibits obvious heterogeneity, and its outcomes extend from arrhythmia to heart failure. Early arrhythmia is a common condition that may progressively become aggravated, and death can occur at any stage of the disease. The most serious complications are heart failure and sudden death(Mc Nally et al., 2013).