Establishment and transcriptome analysis of single blastomerederived cell lines from zebrafish

Maintaining chromosome euploidy in zebrafish embryonic cells is challenging because of the degradation of genomic integrity during cell passaging.In this study,we report the derivation of zebrafish cell lines from single blastomeres.These cell lines have a stable chromosome status attributed to BMP4 and exhibit continuous proliferation in vitro.Twenty zebrafish cell lines are successfully established from single blastomeres.Single-cell transcriptome sequencing analysis confirms the fidelity of gene expression profiles throughout long-term culturing of at least 45 passages.The long-term cultured cells are specialized into epithelial cells,exhibiting similar expression patterns validated by integrative transcriptomic analysis.Overall,this work provides a protocol for establishing zebrafish cell lines from single blastomeres,which can serve as valuable tools for in vitro investigations of epithelial cell dynamics in terms of lifeedeath balance and cell fate determination during normal homeostasis.

CO_2 capture from binary mixture via forming hydrate with the help of tetra-n-butyl ammonium bromide

Hydrate formation rate and separation effect on the capture of CO2 from binary mixture via forming hydrate with 5 wt% tetra-n-butyl ammonium bromide （TBAB） solution were studied. The results showed that the induction time was 5 min, and the hydrate formation process finished in 1 h at 4.5 ℃ and 4.01 MPa. The hydrate formation rate constant reached the maximum of 1.84× 10^-7 molZ/（s.J） with the feed pressure of 7.30 MPa. The CO2 recovery was about 45 % in the feed pressure range from 4.30 to 7.30 MPa. Under the feed pressure of 4.30 MPa, the maximum separation factor and CO2 concentration in hydrate phase were 7.3 and 38.2 mol%, respectively. The results demonstrated that TBAB accelerated hydrate formation and enriched CO2 in hydrate phase under the gentle condition.

CoN_(x)C active sites-rich three-dimensional porous carbon nanofibers network derived from bacterial cellulose and bimetal-ZIFs as efficient multifunctional electrocatalyst for rechargeable Zn–air batteries

In this work, a CoNxC active sites-rich three-dimensional porous carbon nanofibers network derived from bacterial cellulose and bimetal-ZIFs is prepared via a nucleation growth strategy and a pyrolysis process.The material displays excellent electrocatalytic activity for the oxygen reduction reaction, reaching a high limiting diffusion current density of -7.8 mA cm^(-2), outperforming metal–organic frameworks derived multifunctional electrocatalysts, and oxygen evolution reaction and hydrogen evolution reaction with low overpotentials of 380 and 107 mV, respectively. When the electrochemical properties are further evaluated, the electrocatalyst as an air cathode for Zn-air batteries exhibits a high cycling stability for63 h as well as a maximum power density of 308 mW cm^(-2), which is better than those for most Zn-air batteries reported to date. In addition, a power density of 152 mW cm^(-2) is provided by the solid-state Zn-air batteries, and the cycling stability is outstanding for 24 h. The remarkable electrocatalytic properties are attributed to the synergistic effect of the 3 D porous carbon nanofibers network and abundant inserted CoNxC active sites, which enable the fast transmission of ions and mass and simultaneously provide a large contact area for the electrode/electrolyte.

Diagnostic Value of Noninvasive Cardiac Function Measurement for Hypertensive Disorders in Pregnancy

This paper aims to explore the diagnostic value of noninvasive?cardiac function measurement for hypertension in pregnancy. One hundred and fifty-eight cases of gestational hypertension in our hospital were analyzed retrospectively while 30 healthy pregnant women were served as normal controls. The heart function parameters in the two groups were measured with noninvasive cardiac function analyzer, such as myocardial contractive force indexes (JP, VP), impedance indexes (RTM, RS), volume load indexes (GCV, LVDP) and left heart output index (LCI). In all 158 cases of gestational hypertension, the myocardial contractive force indexes, impedance indexes and left heart output index increased unusually. The difference between the two groups was statistically significant (P < 0.05). The myocardial contractive force indexes and the impedance indexes increased significantly higher than left heart output index (P < 0.05). The noninvasive cardiac function measurement was much valuable in the early diagnosis of pregnancy-induced hypertension, especially the myocardial contractive force indexes, impedance indexes and left heart output index, it appeared to be simple and efficient, and it deserves to be clinically popularized and applied.

Unsuccessful letter–sound integration in English reading by native Chinese speakers: evidence from an event related potentials study

Integrating letters and sounds are essential for successful reading in alphabetic languages. It remains unclear if native speakers of non-alphabetic languages integrate letters and sounds in reading an alphabetic lan- guage in the same way as native alphabetic readers do. Chinese is a morpho-syllabic system （each character cor- responds to one syllable） and contrasts sharply with alphabetic languages such as English. Several fMRI studies have shown that native Chinese speakers apply their native language system to read English words. By using the cross- modal mismatch negativity （MMN） paradigm, we directly investigated letter-sound integration for reading in English among native Chinese speakers. To investigate the effect of native language background on letter-sound integration in second language reading, a group of native Korean English learners served as a comparison group. We compared MMN responses between an auditory only condition （only vowels presented） and two audiovisual conditions （AV0, vowel presented synchronously with the corresponding letter; AV200, the letter presented 200 ms before the corresponding vowel） for both native Chinese and native Korean speakers. Native Chinese speakers demonstrated significantly attenuated MMN amplitudes in audiovisual conditions compared with the auditory only condition, regardless of their phonological decoding speed. In con- trast, native Korean speakers showed amplified amplitude MMN in AV200 compared with that in the auditory only condition. The results suggest that native language may shape the brain responses of second language learners to reading a second language in the early stages. Native non- alphabetic language speakers may be unable to use visual information to facilitate their phonological processing in the early stage while naT：lye alphabetic language speakers are capable of integrating letter sounds automatically.

In Situ Super-Hindrance-Triggered Multilayer Cracks for Random Lasing inπ-Functional Nanopolymer Films

In situ self-assembly of semiconducting emitters into multilayer cracks is a significant solution-processing method to fabricate organic high-Q lasers.However,it is still difficult to realize from conventional conjugated polymers.Herein,we create the molecular super-hindrance-etching technology,based on theπ-functional nanopolymer PG-Cz,to modulate multilayer cracks applied in organic single-component random lasers.Massive interface cracks are formed by promoting interchain disentanglement with the super-steric hindrance effect ofπ-interrupted main chains,and multilayer morphologies with photonic-crystal-like ordering are also generated simultaneously during the drop-casting method.Meanwhile,the enhancement of quantum yields on micrometer-thick films(Φ=40%to 50%)ensures high-efficient and ultrastable deep-blue emission.Furthermore,a deep-blue random lasing is achieved with narrow linewidths~0.08 nm and high-quality factors Q≈5,500 to 6,200.These findings will offer promising pathways of organicπ-nanopolymers for the simplification of solution processes applied in lasing devices and wearable photonics.

Derivation of zebrafish heart-related haploid cells

The first long-term cultured haploid embryonic stem cell(ESC)line was established from medaka fish,which represented a breakthrough in vertebrates(Yi et al.,2009).Subsequently,haploid ESC lines were established from mammals and used for genetic analysis and complex genetic manipulation(Elling et al.,2011;Yang et al.,2012).The diploidization of haploid cells is an interesting biological phenomenon and has been widely studied,but the mechanism of diploidization has not yet been fully resolved(Sun et al.,2020).The zebrafish is an excellent vertebrate animal model used in biological studies(Huang et al.,2022).Researchers have isolated many cell lines from zebrafish,such as the zebrafish fibroblast line ZF4(Driever and Rangini,1993).However,there have been no reports regarding stable haploid cell lines of zebrafish,and whether diploidization occurs in zebrafish is unclear.

Informative and diverse emotional conversation generation with variational recurrent pointer-generator

1 Introduction and main contributions The controllable multi-turn emotional response generation task aims to understand the feelings and intentions according to the conversation history between two speakers,and then generate emotionally consistent and empathic responses based on the specific emotion category for smooth human-machine communication[1].The traditional methods[1–3]on this task only focused on enriching the response with desired emotion,which,however,can only generate safe and dull emotional responses.Inspired by the psychology theory on the emotional trigger expressions[4],in this paper,we conjecture that taking the expressions into consideration can refine conversation generation and enhance user satisfaction.Specifically,the emotional trigger expressions can be defined as:the entity-related words and phrases which can provoke and cause the desired emotion of any response.