StSN2 interacts with the brassinosteroid signaling suppressor StBIN2 to maintain tuber dormancy

After harvest,potato tubers undergo an important period of dormancy,which significantly impacts potato quality and seed vigor.StSN2 has been reported as a key gene for maintaining tuber dormancy;in this study,we explored the molecular mechanism by which StSN2 maintains dormancy.StBIN2 was first identified as a candidate protein that interacts with StSN2 by co-immunoprecipitation/mass spectrometry,and both qPCR and enzyme activity experiments showed that StSN2 can promote the StBIN2 expression and activity.In addition,the interaction between StSN2 and StBIN2 was verified by yeast two-hybrid,luciferase complementation experiments and co-immunoprecipitation.Bioinformatics analysis and site-directed mutagenesis confirmed the critical role of cysteine residues of StBIN2 in its binding to StSN2.Similar to that of StSN2,overexpression of StBIN2 extended the dormancy of potato tuber.Interaction between StSN2 and StBIN2 increased the activity of the StBIN2 enzyme,inhibited the expression of StBZR1,and suppressed BR signaling.On the contrary,this interaction promoted the expression of StSnRK2.2/2.3/2.4/2.6 and StABI5,key genes of ABA signaling,and the phosphorylation of StSnRK2.3,thereby promoting ABA signaling.Altogether,our results indicate that StSN2 interacts with StBIN2 through key cysteine residues and StBIN2 maintains tuber dormancy by affecting ABA and BR signaling.Findings of this research offer new insights into the molecular mechanism by which StSN2 maintains potato tuber dormancy through interaction with StSIN2 and provide guidance for potato improvement.

选区激光熔化对GCr15高碳钢成形性能的研

以等离子旋转电极制备的GCr15高碳钢粉末为实验原料,采用选区激光熔化(SLM)制备GCr15高碳轴承钢。研究了SLM成形过程中激光功率、扫描速度、体能量密度对GCr15成形性能的影响和成形后的组织转变。实验结果表明,在参数可调节的范围内,激光功率、扫描速度、体能量密度与相对密度呈线性关系。从能量密度曲线可以看出,功率对相对密度的影响大于扫描速度;在低功率情况下,会出现功率与相对密度关系失真;同时,选区激光熔化制备GCr15轴承钢的组织细小、均匀,主要为粗针状的马氏体和残余奥氏体,随着能量密度的增加,残余奥氏体的含量有所增加。

生物素-亲和素系统的应用研究进展

该文综述了生物素-亲和素系统(Biotin-Avidin System,BAS)相关研究的最新进展,对生物素-亲和素系统在免疫学、蛋白质组学和组织化学等研究领域中的应用进行了梳理和总结,提出了生物素-亲和素系统应用中可能出现的问题,并对生物素-亲和素系统应用前景进行了展望,希望为后续相关研究尤其是在植物科学的研究中提供思路。