Repetitive administration of cultured human CD34+cells improve adenine-induced kidney injury in mice

BACKGROUND There is no established treatment to impede the progression or restore kidney function in human chronic kidney disease(CKD).AIM To examine the efficacy of cultured human CD34+cells with enhanced proliferating potential in kidney injury in mice.METHODS Human umbilical cord blood(UCB)-derived CD34+cells were incubated for one week in vasculogenic conditioning medium.Vasculogenic culture significantly increased the number of CD34+cells and their ability to form endothelial progenitor cell colony-forming units.Adenineinduced tubulointerstitial injury of the kidney was induced in immunodeficient non-obese diabetic/severe combined immunodeficiency mice,and cultured human UCB-CD34+cells were administered at a dose of 1×106/mouse on days 7,14,and 21 after the start of adenine diet.RESULTS Repetitive administration of cultured UCB-CD34+cells significantly improved the time-course of kidney dysfunction in the cell therapy group compared with that in the control group.Both interstitial fibrosis and tubular damage were significantly reduced in the cell therapy group compared with those in the control group(P<0.01).Microvasculature integrity was significantly preserved(P<0.01)and macrophage infiltration into kidney tissue was dramatically decreased in the cell therapy group compared with those in the control group(P<0.001).CONCLUSION Early intervention using human cultured CD34+cells significantly improved the progression of tubulointerstitial kidney injury.Repetitive administration of cultured human UCB-CD34+cells significantly improved tubulointerstitial damage in adenine-induced kidney injury in mice via vasculoprotective and anti-inflammatory effects.

Low-Loss Co₂-Y Ferrites with Added CuO Sintered in Air for High Frequency Application

The sintering characteristics of hexagonal Co2-Y-type ferrite, Ba2Co2Fe12O22, with the addition of 0.6 wt% CuO, were studied in order to allow for preparation in air, as opposed to the conventionally recommended O2, for industrial production. The dependence of the resistivity, ρ magnetic loss, tanδ, and the permeability, μ, at 1 GHz on the sintering temperature was investigated. A low tanδ of 0.05 with a m of 2.7 at a frequency of 1 GHz, along with a high ρ (up to 7 × 104 μm), were attained under sintering at 1170°C in air, which were the same features as those of samples sintered at 1200°C in O2. The dependence of tanδ on grain diameter was also examined, and it was determined that a small grain size (less than 2 μm) is preferable for low tanδ.