Ruthenium complexes which can bind to DNA via electrostatic and intercalation interactions producing strong luminescence have become ideal candidates for DNA staining. However, some of them such as Ru(phen)_3Cl_2 and ...Ruthenium complexes which can bind to DNA via electrostatic and intercalation interactions producing strong luminescence have become ideal candidates for DNA staining. However, some of them such as Ru(phen)_3Cl_2 and Ru(phen)_2(dppz)Cl_2 could hardly cross the cellular membrane of live cells which limited their further interaction with DNA in live cells. To solve this problem, a potential approach is to find a proper vehicle for loading and delivery of these ruthenium complexes into live cells.Mesoporous silica nanoparticles(MSNs) with non-toxicity and good biocompatibility can be good candidates. More importantly,ruthenium complexes with positively charge could be loaded on negatively charged MSNs via electrostatic attractions to form MSNs-Ru hybrid. In vitro test demonstrated that MSNs had no side effects on the interactions between Ru complexes and DNA.Furthermore, it is found that the MSNs-Ru hybrid can enter into living human cervical cancer cells HeLa and stain the DNA while the corresponding ruthenium complexes alone could hardly cross the cellular membrane in the control experiment, demonstrating MSNs can be employed to be an efficient ruthenium complexes delivery nanomaterial for live cell staining.展开更多
The pH values of lysosomes in cancer cells is slightly lower than that in normal cells, which can be used to distinguish cancer cells from normal cells. According to this, a naphthalimide-rhodamine based fluorescent p...The pH values of lysosomes in cancer cells is slightly lower than that in normal cells, which can be used to distinguish cancer cells from normal cells. According to this, a naphthalimide-rhodamine based fluorescent probe（hereafter referred to as RBN） with a pK_a of 4.20 was designed and synthesized for ratiometric sensing of cellular pH via fluorescence resonance energy transfer（FRET）, which can respond to different pH precisely through ratiometric fluorescence intensity（Ⅰ_（577）/Ⅰ_（540））. RBN can be employed to distinguish cancer cells from normal cells on the basis of different fluorescent response, in particular, RBN showed excellent water solubility and low cell toxicity, all these are quite significant for potential application in cancer diagnose and therapy.展开更多
Based on the reaction between the azido group and hydrogen sulfide （H2S）, BTN, an ofT-on fluorescent sensor was synthesized, which exhibited a 20-fold fluorescence enhancement upon addition of H2S, and a 5.6×10...Based on the reaction between the azido group and hydrogen sulfide （H2S）, BTN, an ofT-on fluorescent sensor was synthesized, which exhibited a 20-fold fluorescence enhancement upon addition of H2S, and a 5.6×10 8 mol/L detection limit can be reached in 10 min. To check its applicability, an easy prepared test paper strip of BTN was made for rapid and selective detection of hydrogen sulfide in red-wine samples, the detection limit is as low as 1 μmol/L.展开更多
Adenosine 5＇-triphosphate（ATP） not only participates in various physiological activities as the universal energy currency but also implicates in various pathological processes in living cells. Consequently,sensitiv...Adenosine 5＇-triphosphate（ATP） not only participates in various physiological activities as the universal energy currency but also implicates in various pathological processes in living cells. Consequently,sensitive and selective detection ATP in live cells, tissues, as well as environmental samples, are urgently demanded. Due to the simple and convenient operation, economy cost, high selectivity for analyte, well biocompatibility and low cytotoxicity, fluorescent sensors for monitoring ATP have aroused great attention of researchers. In recent years, a large number of fluorescent sensors for detecting ATP have developed. This manuscript summarized most of these sensors and the interaction-mechanism between ATP and sensors, mainly including electrostatic interaction, p-p interaction, covalent bonding or hydrogen bond, or combinations of them, and the advantages of each strategy were also generalized.Here, a viewpoint of classification was shown where the sensors were divided into five typed ones according to the structure of probes used.展开更多
基金supported by the Scientific Research Foundation of Northwest A&F University (Z111021103, Z111021107)the National Natural Science Foundation of China (21472016, 21272030, 21476185)
文摘Ruthenium complexes which can bind to DNA via electrostatic and intercalation interactions producing strong luminescence have become ideal candidates for DNA staining. However, some of them such as Ru(phen)_3Cl_2 and Ru(phen)_2(dppz)Cl_2 could hardly cross the cellular membrane of live cells which limited their further interaction with DNA in live cells. To solve this problem, a potential approach is to find a proper vehicle for loading and delivery of these ruthenium complexes into live cells.Mesoporous silica nanoparticles(MSNs) with non-toxicity and good biocompatibility can be good candidates. More importantly,ruthenium complexes with positively charge could be loaded on negatively charged MSNs via electrostatic attractions to form MSNs-Ru hybrid. In vitro test demonstrated that MSNs had no side effects on the interactions between Ru complexes and DNA.Furthermore, it is found that the MSNs-Ru hybrid can enter into living human cervical cancer cells HeLa and stain the DNA while the corresponding ruthenium complexes alone could hardly cross the cellular membrane in the control experiment, demonstrating MSNs can be employed to be an efficient ruthenium complexes delivery nanomaterial for live cell staining.
基金supported by the National Natural Science Foundation of China (Nos. 21272030, 21472016, 21306019, 21576042)
文摘The pH values of lysosomes in cancer cells is slightly lower than that in normal cells, which can be used to distinguish cancer cells from normal cells. According to this, a naphthalimide-rhodamine based fluorescent probe（hereafter referred to as RBN） with a pK_a of 4.20 was designed and synthesized for ratiometric sensing of cellular pH via fluorescence resonance energy transfer（FRET）, which can respond to different pH precisely through ratiometric fluorescence intensity（Ⅰ_（577）/Ⅰ_（540））. RBN can be employed to distinguish cancer cells from normal cells on the basis of different fluorescent response, in particular, RBN showed excellent water solubility and low cell toxicity, all these are quite significant for potential application in cancer diagnose and therapy.
基金This work was financially supported by the Scien- tific Research Foundation of Northwest A＆F University （Nos. Z111021103 and Z111021107）, the National Nat- ural Science Foundation of China （Nos. 21206137, 21272030, 201205095, 21472016）, and Shaanxi Prov- ince Science and Technology （No. 2013K12-03-23）.
文摘Based on the reaction between the azido group and hydrogen sulfide （H2S）, BTN, an ofT-on fluorescent sensor was synthesized, which exhibited a 20-fold fluorescence enhancement upon addition of H2S, and a 5.6×10 8 mol/L detection limit can be reached in 10 min. To check its applicability, an easy prepared test paper strip of BTN was made for rapid and selective detection of hydrogen sulfide in red-wine samples, the detection limit is as low as 1 μmol/L.
基金supported by the National Natural Science Foundation of China (Nos. 21676218, 21476185, 21472016, 21272030)the Fundamental Research Funds for the Central Universities (Nos. 2014YB027, 2452015447, 2452013py014)Shaanxi Province Science and Technology
文摘Adenosine 5＇-triphosphate（ATP） not only participates in various physiological activities as the universal energy currency but also implicates in various pathological processes in living cells. Consequently,sensitive and selective detection ATP in live cells, tissues, as well as environmental samples, are urgently demanded. Due to the simple and convenient operation, economy cost, high selectivity for analyte, well biocompatibility and low cytotoxicity, fluorescent sensors for monitoring ATP have aroused great attention of researchers. In recent years, a large number of fluorescent sensors for detecting ATP have developed. This manuscript summarized most of these sensors and the interaction-mechanism between ATP and sensors, mainly including electrostatic interaction, p-p interaction, covalent bonding or hydrogen bond, or combinations of them, and the advantages of each strategy were also generalized.Here, a viewpoint of classification was shown where the sensors were divided into five typed ones according to the structure of probes used.
