甲状旁腺素对人甲状腺髓样癌细胞增殖抑制的实验研究(英文)

Objective: The purpose of the study was to investigate the effects of parathyroid hormone and parathyroid hormone receptor monoclonal antibody on in vitro growth and proliferation of human medullary thyroid carcinoma cell lines. Methods: The medullary thyroid carcinoma cell line was cultured in vitro, with parathyroid hormone and parathyroid hormone receptor monoclonal antibody treatment intervention, the growth of the cells was observed under an inverted contrast microscope, the MTT assay was used to detect the cell growth inhibition rate. Results: Under the inverted contrast microscope, the cells changed significantly, the parathyroid hormone and parathyroid hormone receptor monoclonal antibodies can effectively inhibit the proliferation of medullary thyroid cancer cells in a time and dose dependent. When parathyroid hormone concentration reached a concentration of 2.0 μmol/L, the parathyroid hormone receptor monoclonal antibody reached a concentration of 1.0 μmol/L, the cell growth was most significantly inhibited(P < 0.05). Conclusion: Parathyroid hormone and parathyroid hormone receptor monoclonal antibody were able to inhibit the proliferation of medullary thyroid carcinoma cells and significantly reduce the proliferation index.

结节性甲状腺肿伴发胆囊结石与甲状腺功能的相关性(英文)

Objective: The purpose of the study wass to explore the correlation between thyroid function and nodular goiter accompanied with gallstone. Methods: We collected 120 cases about nodular goiter accompanied with gallstone and 128 cases about nodular goiter and establish 50 healthy control groups. Detected t level of hyrotropic hormone(TSH), total triiodothyronine(TT3), total thyroxine in the peripheral venous blood of these cases in the three groups by using electrochemiluminescence immunoassay, measure level of total cholesterol(TC), high-density lipoprotein cholesterol(HDL-C), low-density lipoprotein cholesterol(LDL-C) and total bile acid(TBA) levels by using enzymic method, and observed the changes of thyroid function and blood lipid among the three groups. Results: The serum TT3 level in nodular goiter accompanied with gallstone group and the nodular goiter group was significantly lower than that in control group(P < 0.01), and TSH level in the nodular goiter accompanied with gallstone group is significantly higher than that in control group(P < 0.01). There were no statistical significance about difference of TT4 level among the three groups(P > 0.05). Accordingly, TC and LDL-C level in nodular goiter accompanied with gallstone group was significantly higher than that in nodular goiter and control group(P < 0.01), while TBA level in nodular goiter accompanied with gallstone group was significantly lower than that in simple nodular goiter group and control group(P < 0.01). There was no statistical significance about difference of TC and LDL-C level between simple nodular goiter group and control group(P > 0.05). The HDL-C level in nodular goiter accompanied with gallstone group and control group was higher than that in simple nodular goiter group(P < 0.01). Conclusion: The originating etiologic factor of nodular goiter accompanied with gallstone may be related to that the decreased TT3 induced sub-clinical hypothyroidism.

甲状腺癌钠、碘转运蛋白与血清β_2-MG变化的相关性分析(英文)

Objective: The aim of our study was to investigate the correlation between the expression of sodium/iodide symporter, serum levels of β2-MG and prognosis of thyroid carcinoma patients. Methods: Ninty-five cases with thyroid carcinoma, using enzyme-linked immunosorbent assay with double-antibody sandwich to detect the serum β2-MG levels and immunohistochemistry to detect NIS expression of thyroid cancer tissue. Results: Thirty-seven cases showed positive expression of sodium/iodide symporter (38.9%) and 30 cases showed positive expression of β2-MG (31.57%). There were significant differences of NIS expression (χ2=8.207, P=0.017) and β2-MG expression (χ2=10.121, P=0.006) between different pathological types of thyroid carcinoma, but there was no correlation between the positive rate of the two research groups (r=-0.546, P=0.633). The significant differences was observed in expression of sodium/iodide symporter (χ2 = 9.272, P=0.002) and expression of β2-MG (χ2=4.441, P=0.035) between the group with neck lymph node metastasis and the group without neck lymph node metastasis and both positive rate was significantly negatively correlated (r=1.000, P=0.000). The significant differences was observed in expression of sodium/iodide symporter (χ2=9.272, P=0.002) and expression of β2-MG (χ2 = 3.867, P=0.043) between the group with distant organ metastasis and the group without distant organ metastasis (χ2=11.985, P=0.001) and both positive rate was significantly negatively correlated (r=-1.000, P=0.000). Conclusion: There are significantly negatively correlated between neck lymph node metastasis, distant organ metastasis and expression of sodium/iodide symporter and expression of β2-MG. Thyroid cancer lymph node and distant organ metastasis, the tumor tissue NIS expression and serum levels of β2-MG is significantly negatively correlated. The detection of serum β2-MG provides clinical reference value for the effects on radionuclide therapy and prognosis assessment of thyroid carcinoma. Serum β2-MG levels is negatively correlated with prognosis in thyroid cancer patients.

甲状腺癌血清β_2-MG临床意义评估(英文)

Objective:The aim of this study was to investigate the clinical value and relevance on the serum β2-microglobulin(β2-MG) of patients with thyroid cancer.Methods:One thousand and two normal cases,95 thyroid cancer patients and 243 nodular goiter patients were selected to measure serum β2-MG levels using double-antibody sandwich enzyme-linked immunosorbent assay(ELISA).Results:The positive rate of 7.78% in normal population(78/1002) and 31.57% in thyroid cancer patients(30/95).There were significant differences between the normal population and thyroid cancer patients(χ2 = 55.352;P = 0.000).The positive rate of 7.81% in nodular goiter patients(19/243) and there were no significant differences between the normal population and nodular goiter patients(χ2 = 0.0004;P = 0.986),but significant differences between nodular goiter patients and thyroid cancer patients(χ2 = 31.106;P = 0.000).Meanwhile,the significant difference of the positive rate existed in between the various pathological types of thyroid cancer(χ2 = 10.015;P = 0.007),anaplastic thyroid cancer patients with the highest positive rate and The significant difference was found between the positive lymph node metastasis groups and negative lymph node metastasis groups(χ2 = 4.441;P = 0.035),the presence of distant metastasis group and absence of distant metastasis group(χ2 = 9.795;P = 0.002).Conclusion:Serum β2-MG levels and prognosis of thyroid cancer patients was negatively correlated.It showed important clinical value to detect the level of β2-MG in the early diagnosis,prognosis and the clinical observation for thyroid cancer patients.

Antifungal Activity of Schinifoline Against Candida Albicans in Caenorhabditis Elegans

Zanthoxylum schinifolium has been used as spices and traditional medicine in China for hundreds of years.A variety of active substances have been isolated from Zanthoxylum schinifolium using biological and chemical techniques.Among these substances,the effect of schinifoline has gradually attracted much attention.Candida albicans is one of the most common pathogens isolated from the gastrointestinal tract,vagina,and mouth in healthy individuals.In a healthy population,there are various mechanisms in host,such as the microbial flora,the epithelial barriers,and the innate immune system,that can control the presence of Candida albicans.However,when host immunity is compromised,an invasive fungal infection is more likely to occur.In this study,we explored the antifungal activity of schinifoline against Candida albicans in Caenorhabditis elegans.To determine the optimal concentration of schinifoline,we investigated the lifespan,defecation cycle and locomotion behavior of Caenorhabditis elegans after treatment with schinifoline.In addition,we examined colony formation in the intestine of Caenorhabditis elegans after Candida albicans infection.The results indicated that 100 and 200 mg/L of schinifoline could prolonged the lifespan,shorten the defecation cycle and increased the locomotion behavior of Caenorhabditis elegans,with 100 mg/L of schinifoline being the optimal concentration.Moreover,100 mg/L of schinifoline increased the lifespan of Caenorhabditis elegans after infection and inhibited the colony formation of Candida albicans in Caenorhabditis elegans intestine.Therefore,we concluded that schinifoline exhibits anti-fungal effects and its potential use as natural drugs should be further explored in future studies.

应用于深井脉冲源装备的有序堆积相变胶囊热防护数值研究

针对深井恶劣环境下脉冲功率源装备内电子元件的热防护问题,本文利用数值方法对比了多种有序堆积相变胶囊复合结构的热防护性能,探究了相变胶囊尺寸、堆积方式等对热防护性能的影响,提出了热流密度评价指标与内壁温度数学预测模型.结果表明,相变微胶囊-玻璃棉复合结构具有较好的热防护性能,内壁温上升速率相较于玻璃棉填充结构可降低13.8%,但相变材料的含量制约着热防护效果;使用大尺寸的相变胶囊,增大相变材料比例的同时可形成封闭气孔,相比于相变微胶囊-玻璃棉复合结构,内壁温升幅度进一步降低了19.3%.通过改善相变胶囊堆积结构,发现采用大尺寸相变胶囊单层阵列结构的热防护性能更优.最后,提出了相变胶囊单层阵列热防护结构下内壁温升预测公式,预测值与数值模拟结果的平均误差低于7%.