Cardiovascular disease is the leading cause of global mortality,with anticoagulant therapy being the main prevention and treatment strategy.Recombinant hirudin(r-hirudin)is a direct thrombin inhibitor that can potenti...Cardiovascular disease is the leading cause of global mortality,with anticoagulant therapy being the main prevention and treatment strategy.Recombinant hirudin(r-hirudin)is a direct thrombin inhibitor that can potentially prevent thrombosis via subcutaneous(SC)and intravenous(IV)administration,but there is a risk of haemorrhage via SC and IV.Thus,microneedle(MN)provides painless and sanitary alternatives to syringes and oral administration.However,the current technological process for the micro mould is complicated and expensive.The micro mould obtained via three-dimensional(3D)printing is expected to save time and cost,as well as provide a diverse range of MNs.Therefore,we explored a method for MNs array model production based on 3D printing and translate it to micro mould that can be used for fabrication of dissolving MNs patch.The results show that r-hirudin-loaded and hyaluronic acid(HA)-based MNs can achieve transdermal drug delivery and exhibit significant potential in the prevention of thromboembolic disease without bleeding in animal models.These results indicate that based on 3D printing technology,MNs combined with r-hirudin are expected to achieve diverse customizableMNs and thus realize personalized transdermal anticoagulant delivery for minimally invasive and long-term treatment of thrombotic disease.展开更多
Pyrogallic acid(PG)was used as a modeling carbon source in fabricating nano-structured hollow carbon materials(HCMs)by a chemical vapor deposition(CVD)method.We found that non-isothermal deposition can improve the int...Pyrogallic acid(PG)was used as a modeling carbon source in fabricating nano-structured hollow carbon materials(HCMs)by a chemical vapor deposition(CVD)method.We found that non-isothermal deposition can improve the integrity of the obtained HCMs.The different pyrolyzed species from PG under varied temperatures lead to the temperature-dependent deposition yield,graphitization degree and morphology of the HCMs.HCMs including hollow spheres of varied sizes,cubic boxes with yolk-shell structure,nanotubes,mesoporous particles and double-shelled fibers,were prepared by using different templates,demonstrating the universality of this strategy.The carbon source has been extended to other plant polyphenols.The abundant and renewable solid precursors for CVD method endow this strategy excellent operation safety,improved storage and transportation convenience mnd low cost,mnd would boost the production of morphology-and size-controlled HCMs and their applications in the fields such as water treatment,electrode materials,adsorbent,drug delivery,and so forth.展开更多
基金supported by the National Natural Science Foundation of China (NSFC 81902995)the project funded by China Postdoctoral Science Foundation (2018M641936)
文摘Cardiovascular disease is the leading cause of global mortality,with anticoagulant therapy being the main prevention and treatment strategy.Recombinant hirudin(r-hirudin)is a direct thrombin inhibitor that can potentially prevent thrombosis via subcutaneous(SC)and intravenous(IV)administration,but there is a risk of haemorrhage via SC and IV.Thus,microneedle(MN)provides painless and sanitary alternatives to syringes and oral administration.However,the current technological process for the micro mould is complicated and expensive.The micro mould obtained via three-dimensional(3D)printing is expected to save time and cost,as well as provide a diverse range of MNs.Therefore,we explored a method for MNs array model production based on 3D printing and translate it to micro mould that can be used for fabrication of dissolving MNs patch.The results show that r-hirudin-loaded and hyaluronic acid(HA)-based MNs can achieve transdermal drug delivery and exhibit significant potential in the prevention of thromboembolic disease without bleeding in animal models.These results indicate that based on 3D printing technology,MNs combined with r-hirudin are expected to achieve diverse customizableMNs and thus realize personalized transdermal anticoagulant delivery for minimally invasive and long-term treatment of thrombotic disease.
基金the financial supports from the Chinese Academy of Sciences(No.QYZDB-SSW-SLH025)the National Natural Science Foundation of China(Nos.51673203,21474117,51522308)Shanghai Research Institute of Chemical Industry Co.,Ltd.(SKL-LCTP-201801).
文摘Pyrogallic acid(PG)was used as a modeling carbon source in fabricating nano-structured hollow carbon materials(HCMs)by a chemical vapor deposition(CVD)method.We found that non-isothermal deposition can improve the integrity of the obtained HCMs.The different pyrolyzed species from PG under varied temperatures lead to the temperature-dependent deposition yield,graphitization degree and morphology of the HCMs.HCMs including hollow spheres of varied sizes,cubic boxes with yolk-shell structure,nanotubes,mesoporous particles and double-shelled fibers,were prepared by using different templates,demonstrating the universality of this strategy.The carbon source has been extended to other plant polyphenols.The abundant and renewable solid precursors for CVD method endow this strategy excellent operation safety,improved storage and transportation convenience mnd low cost,mnd would boost the production of morphology-and size-controlled HCMs and their applications in the fields such as water treatment,electrode materials,adsorbent,drug delivery,and so forth.
