SUMOylation is an impo^ant post-translational modification process that regulates different cellular functions in eukaryotes. SIZ/PIAS-type SAP and Mizl （SIZ0 proteins exhibit SUMO E3 ligase activity, which modulates...SUMOylation is an impo^ant post-translational modification process that regulates different cellular functions in eukaryotes. SIZ/PIAS-type SAP and Mizl （SIZ0 proteins exhibit SUMO E3 ligase activity, which modulates SUMOylation. However, SIZI in tomato has been rarely investigated. In this study, a tomato SIZI gene （SISIZI） was isolated and its molecular characteristics and role in tolerance to drought stress are described. SISIZI was upregulated by cold, sodium chloride （NaCI）, polyethylene glycol （PEG）, hydrogen peroxide （H20~） and abscisic acid （ABA）, and the corresponding proteins were localized in the nucleus. The expression of SISIZI in Arabidopsis thaliana （Arabidopsis） sizl-2 mutants partially complemented the phenotypes of dwarf, cold sensitivity and ABA hypersensitivity. SISIZI also exhibited the activity of SUMO E3 ligase to promote the accumulation of SUMO conjugates. Under drought stress, the ectopic expression of SISIZl in transgenic tobacco lines enhanced seed germination and reduced the accumulation of reactive oxygen species..SISIZl overexpression conferred the plants with improved growth, high free proline content, minimal malondialdehyde accumulation and increased accumulation of SUMO conjugates. SISIZ1 is a functional homolog of Arabidopsis SIZ1 with SUMO E3 ligase activity. Therefore, overexpression of SISIZ1 enhanced the tolerance of transgenic tobacco to drought stress.展开更多
A substation planning method that accounts for the widespread introduction of distributed generators(DGs)in a low-carbon economy is proposed.With the proliferation of DGs,the capacity that DGs contribute to the distri...A substation planning method that accounts for the widespread introduction of distributed generators(DGs)in a low-carbon economy is proposed.With the proliferation of DGs,the capacity that DGs contribute to the distribution network has become increasingly important.The capacity of a DG is expressed as a capacity credit(CC)that can be evaluated according to the principle that the reliability index is unchanged before and after the introduction of the DG.A method that employs a weighted Voronoi diagram is proposed for substation planning considering CC.A low-carbon evaluation objective function is added to the substation planning model to evaluate the contribution of DGs to a low-carbon economy.A case study is analyzed to demonstrate the practicality of the proposed method.展开更多
基金supported by the State Key Basic Research and Development Plan of China(2015CB150105)the Natural Science Foundation of China(31171474,31371553)
文摘SUMOylation is an impo^ant post-translational modification process that regulates different cellular functions in eukaryotes. SIZ/PIAS-type SAP and Mizl （SIZ0 proteins exhibit SUMO E3 ligase activity, which modulates SUMOylation. However, SIZI in tomato has been rarely investigated. In this study, a tomato SIZI gene （SISIZI） was isolated and its molecular characteristics and role in tolerance to drought stress are described. SISIZI was upregulated by cold, sodium chloride （NaCI）, polyethylene glycol （PEG）, hydrogen peroxide （H20~） and abscisic acid （ABA）, and the corresponding proteins were localized in the nucleus. The expression of SISIZI in Arabidopsis thaliana （Arabidopsis） sizl-2 mutants partially complemented the phenotypes of dwarf, cold sensitivity and ABA hypersensitivity. SISIZI also exhibited the activity of SUMO E3 ligase to promote the accumulation of SUMO conjugates. Under drought stress, the ectopic expression of SISIZl in transgenic tobacco lines enhanced seed germination and reduced the accumulation of reactive oxygen species..SISIZl overexpression conferred the plants with improved growth, high free proline content, minimal malondialdehyde accumulation and increased accumulation of SUMO conjugates. SISIZ1 is a functional homolog of Arabidopsis SIZ1 with SUMO E3 ligase activity. Therefore, overexpression of SISIZ1 enhanced the tolerance of transgenic tobacco to drought stress.
基金This work was supported by the National Natural Science Foundation of China(No.51477116).
文摘A substation planning method that accounts for the widespread introduction of distributed generators(DGs)in a low-carbon economy is proposed.With the proliferation of DGs,the capacity that DGs contribute to the distribution network has become increasingly important.The capacity of a DG is expressed as a capacity credit(CC)that can be evaluated according to the principle that the reliability index is unchanged before and after the introduction of the DG.A method that employs a weighted Voronoi diagram is proposed for substation planning considering CC.A low-carbon evaluation objective function is added to the substation planning model to evaluate the contribution of DGs to a low-carbon economy.A case study is analyzed to demonstrate the practicality of the proposed method.
