Gastric ulcer is a global health concern nowadays. Adinandra nitida, known as Shibi tea, is a flavonoidrich plant found in South China. A. nitida possesses many healthy properties, such as antioxidation and reducing b...Gastric ulcer is a global health concern nowadays. Adinandra nitida, known as Shibi tea, is a flavonoidrich plant found in South China. A. nitida possesses many healthy properties, such as antioxidation and reducing blood pressure. However, its effects on gastric ulcer have not been investigated. This study aimed to investigate the effects of the Shibi tea water extract(STE) and its main flavonoid camellianin A(CA) in hydrochloric acid(HCl) and ethanol(Et OH)-induced acute gastric ulcer in mice. Administration of CA and STE for continuous two days after stimulation by HCl/Et OH significantly attenuated the deterioration of gastric mucosal damage by lowering the gross gastric mucosal index, histopathological injury index, the oxidative stress, the expression of the inflammatory cytokines TNF-α and IL-6, and the expression of inflammatory mediators i NOS and COX-2. Western blotting and immunohistochemistry analysis showed that CA and STE regulated the inflammatory signaling pathway protein levels of IκB-α and NF-κB. Taken together, our study verified that CA and STE have antioxidative and anti-inflammatory effects in gastric ulcer mice. We propose that A. nitida should be developed as natural functional food for acute gastric ulcer patients base on the gastroprotective effects of STE and its main flavonoid CA.展开更多
Objective:In this study,black tea and Citrus maxima(BT-CM),yellow tea and C.maxima(YT-CM),green tea and C.maxima(GT-CM)as subjects,the active ingredient content and antioxidant activity of three tea and C.maxima(T-CM)...Objective:In this study,black tea and Citrus maxima(BT-CM),yellow tea and C.maxima(YT-CM),green tea and C.maxima(GT-CM)as subjects,the active ingredient content and antioxidant activity of three tea and C.maxima(T-CM)were analyzed.The effects of three T-CMs on apoptosis of liver cells in vitro and its mechanism were further explored.Methods:National standard method and HPLC were used for active ingredient analysis.MTT,cell fow cytometry and Western blot were used to analyze the effects of three T-CMs on cell proliferation,apoptosis,and its underlying molecular mechanism.Results:The content of tea polyphenols,free amino acids,ratio of polyphenols and amino acids,ester catechins,non-ester catechins and caffeine in YT-CM and GT-CM was significantly higher than that of BT-CM.The in vitro antioxidant capacity of YT-CM and GT-CM was also significantly stronger than that of BT-CM.Three T-CMs had the effects of inhibiting proliferation,arresting cell cycle and inducing apoptosis in HepG2 and Bel7402 cells,especially YT-CM and GT-CM.Western blot analysis showed three T-CMs activated PI3K/AKT/mTOR signaling pathway and regulated the expression levels of apoptosis-related proteins Bax,Bcl-2 and Caspase-3/9.YT-CM and GT-CM had better ability to change the signal pathway than BT-CM.Conclusion:In short,T-CMs,which combined different degrees of fermentation tea with C.maxima,were rich in nutrients and biologically active substances.T-CMs,especially YT-CM and GT-CM,are healthy drinks that help to prevent and treat liver cancer.展开更多
The morphology of bone repair materials,such as particle size and roughness of the materials surface,can affect the adsorption of protein molecules.The effects of surface morphology on the absorption of proteins with ...The morphology of bone repair materials,such as particle size and roughness of the materials surface,can affect the adsorption of protein molecules.The effects of surface morphology on the absorption of proteins with different charges were studied.Submicron and nano hydroxyapatite(HA)powders prepared by the chemical precipitation method were coated on the surface of a gold sheet by electrophoretic deposition.Various hydroxyapatite coating morphologies were obtained by controlling the powder particle size and the deposition time.The coating surface morphology was analyzed by Atomic Force Microscopy(AFM),and the adsorption behavior of differently charged proteins on the surface was dynamically monitored by Quartz Crystal Microbalance with Dissipation(QCM-D).The adsorption dependence of two proteins with different charges upon hydroxyapatite coating surface morphology was investigated.Results show that coating surfaces with smaller deposited particle sizes are favorable for the adsorption of negatively-charged bovine albumin,while with larger deposited particles facilitate the adsorption of positively-charged lysozyme.This may be because that the negatively-charged hydroxyapatite coating exhibits stronger electrostatic effect as the increase in the coating particle size,which facilitates the adsorption of positively-charged proteins and hinders the adsorption of negatively-charged proteins.Increasing coating surface roughness facilitates protein adsorption,though the particle size exhibits a dominant influence.These results are significant for selective adsorption of proteins on material surfaces.展开更多
基金Foundation item： Supported by the National Natural Science Foundation of China （Grant Nos. 11302057, 11302056）, the Fundamental Research Funds for the Central Universities （Grant No. HEUCF140115） and the Research Funds for State Key Laboratory of Ocean Engineering in Shanghai Jiao Tong University （Grant No. 1310）.
基金Foundation item： Supported by the Lloyd＇s Register Foundation, the Fundamental Research Funds for the Central Universities （Gram No. HEUCF140115）, the National Natural Science Foundation of China （11102048, 11302057）, the Research Funds for State Key Laboratory of Ocean Engineering in Shanghai Jiao Tong University （Grant No. 1310）, and the Specialized Research Fund for the Doctoral Program of Higher Education of China （20132304120028）.
基金funded by the “14th Five-Year Plan” team-building projects of Guangdong Academy Agricultural Sciences (202126TD)National Natural Science Foundation of China (81903319, 81803236, 31800295)+10 种基金Guangdong Science and Technology program (2018KJYZ002)Guangdong Basic and Applied Basic Research Foundation (2020A1515011266)Qingyuan Science and Technology Program (DZXQY021, 181022114566189, 181022114566189, 2019A039, 2020KJJH042)Shaoguan Science and Technology Program (2018CS11902, 2018sn081)Guangzhou Science and Technology Program (202002030202)Zhaoqing Science and Technology Program (2019N001, 2019N013)Maoming Science and Technology Program (mmkj2020045)Zhanjiang Science and Technology Program (2020A03014)Yingde Science and Technology Board (JHXM2018029)Innovation Fund projects of Guangdong Academy of Agricultural Sciences (202115)Special fund for scientific innovation strategy-construction of high level Academy of Agriculture Science (R2019PY-JX004, R2018YJ-YB3002, R2016YJYB3003, R2018PY-QF005, R2018QD-101)。
文摘Gastric ulcer is a global health concern nowadays. Adinandra nitida, known as Shibi tea, is a flavonoidrich plant found in South China. A. nitida possesses many healthy properties, such as antioxidation and reducing blood pressure. However, its effects on gastric ulcer have not been investigated. This study aimed to investigate the effects of the Shibi tea water extract(STE) and its main flavonoid camellianin A(CA) in hydrochloric acid(HCl) and ethanol(Et OH)-induced acute gastric ulcer in mice. Administration of CA and STE for continuous two days after stimulation by HCl/Et OH significantly attenuated the deterioration of gastric mucosal damage by lowering the gross gastric mucosal index, histopathological injury index, the oxidative stress, the expression of the inflammatory cytokines TNF-α and IL-6, and the expression of inflammatory mediators i NOS and COX-2. Western blotting and immunohistochemistry analysis showed that CA and STE regulated the inflammatory signaling pathway protein levels of IκB-α and NF-κB. Taken together, our study verified that CA and STE have antioxidative and anti-inflammatory effects in gastric ulcer mice. We propose that A. nitida should be developed as natural functional food for acute gastric ulcer patients base on the gastroprotective effects of STE and its main flavonoid CA.
基金supported by the"14th Five-Year Plan"teambuilding projects of Guangdong Academy of Agricultural Sciences[202126TD]Guangdong Basic and Applied Basic Research Foundation[2020A1515011266,2021A1515010958]+3 种基金Guangzhou Science and Technology Plan Projects[202002030202,202102020047]Key-Area Research and Development Program of Guangdong Province[2020B0202080003]Innovation Fund projects of Guangdong Academy of Agricultural Sciences[202115,202035]Special fund for scientific innovation strategy-construction of high level Academy of Agriculture Science[R2019PY-JX004]
文摘Objective:In this study,black tea and Citrus maxima(BT-CM),yellow tea and C.maxima(YT-CM),green tea and C.maxima(GT-CM)as subjects,the active ingredient content and antioxidant activity of three tea and C.maxima(T-CM)were analyzed.The effects of three T-CMs on apoptosis of liver cells in vitro and its mechanism were further explored.Methods:National standard method and HPLC were used for active ingredient analysis.MTT,cell fow cytometry and Western blot were used to analyze the effects of three T-CMs on cell proliferation,apoptosis,and its underlying molecular mechanism.Results:The content of tea polyphenols,free amino acids,ratio of polyphenols and amino acids,ester catechins,non-ester catechins and caffeine in YT-CM and GT-CM was significantly higher than that of BT-CM.The in vitro antioxidant capacity of YT-CM and GT-CM was also significantly stronger than that of BT-CM.Three T-CMs had the effects of inhibiting proliferation,arresting cell cycle and inducing apoptosis in HepG2 and Bel7402 cells,especially YT-CM and GT-CM.Western blot analysis showed three T-CMs activated PI3K/AKT/mTOR signaling pathway and regulated the expression levels of apoptosis-related proteins Bax,Bcl-2 and Caspase-3/9.YT-CM and GT-CM had better ability to change the signal pathway than BT-CM.Conclusion:In short,T-CMs,which combined different degrees of fermentation tea with C.maxima,were rich in nutrients and biologically active substances.T-CMs,especially YT-CM and GT-CM,are healthy drinks that help to prevent and treat liver cancer.
基金This work was financially supported by the National Natural Science Foundation of China(Grant Nos.51972120 and 51772105).
文摘The morphology of bone repair materials,such as particle size and roughness of the materials surface,can affect the adsorption of protein molecules.The effects of surface morphology on the absorption of proteins with different charges were studied.Submicron and nano hydroxyapatite(HA)powders prepared by the chemical precipitation method were coated on the surface of a gold sheet by electrophoretic deposition.Various hydroxyapatite coating morphologies were obtained by controlling the powder particle size and the deposition time.The coating surface morphology was analyzed by Atomic Force Microscopy(AFM),and the adsorption behavior of differently charged proteins on the surface was dynamically monitored by Quartz Crystal Microbalance with Dissipation(QCM-D).The adsorption dependence of two proteins with different charges upon hydroxyapatite coating surface morphology was investigated.Results show that coating surfaces with smaller deposited particle sizes are favorable for the adsorption of negatively-charged bovine albumin,while with larger deposited particles facilitate the adsorption of positively-charged lysozyme.This may be because that the negatively-charged hydroxyapatite coating exhibits stronger electrostatic effect as the increase in the coating particle size,which facilitates the adsorption of positively-charged proteins and hinders the adsorption of negatively-charged proteins.Increasing coating surface roughness facilitates protein adsorption,though the particle size exhibits a dominant influence.These results are significant for selective adsorption of proteins on material surfaces.
