Objective:To study the perfusion CT features of rabbit VX2 brain tumor with correlation to MVD and VEGF,and to validate perfusion CT for reflection of tumor angiogenesis.Methods:Rabbit VX2 brain tumor model was establ...Objective:To study the perfusion CT features of rabbit VX2 brain tumor with correlation to MVD and VEGF,and to validate perfusion CT for reflection of tumor angiogenesis.Methods:Rabbit VX2 brain tumor model was established by injection of 100 μL viable tumor cells(107/mL)through a 2 mm-hole 5 mm to the right of the sagittal suture and 5 mm posterior to the coronal suture bored by dental drill.MRI was performed every 2 days after seven days of implantation to evaluate the growth of the tumor.Twenty New Zealand White rabbits with tumor size over 3 mm in diameter were randomly divided into 2 groups according to the tumor growth time with those less than 3 weeks as group 1 and those more than 3 weeks as group 2,and perfusion CT were performed accordingly.CT measurements of BV,BF and PS from tumor,peritumor and contralateral normal tissue regions were obtained.After that the animals were sacrificed and 2% Evans blue(2 mL/kg)was given intravenously in 16 of these animals 1 h prior to sacrifice to detect breakdown of the blood brain barrier.VEGF and MVD were evaluated in im-munohistochemical examination of the specimens.Results:Tumor had significantly higher BV,BF and PS(P=0.000)than peritumor and normal tissue region.Tumor BV,BF and MVD in group 2 were significantly higher than that in group 1(P<0.01).Significant linear correlation was found between MVD and BV(r=0.915,P=0.000),MVD and BF(r=0.901,P=0.000),and MVD and PS(r=0.459,P=0.042).We also found a rank correlation between PS and blue stain of tumor(rs=0.861,P=0.000).Conclu-sion:Perfusion CT can distinguish tumor from peritumor and normal tissue clearly,reflect tumor angiogenesis accurately,and provide useful information for the evaluation of brain tumor.展开更多
文摘Objective:To study the perfusion CT features of rabbit VX2 brain tumor with correlation to MVD and VEGF,and to validate perfusion CT for reflection of tumor angiogenesis.Methods:Rabbit VX2 brain tumor model was established by injection of 100 μL viable tumor cells(107/mL)through a 2 mm-hole 5 mm to the right of the sagittal suture and 5 mm posterior to the coronal suture bored by dental drill.MRI was performed every 2 days after seven days of implantation to evaluate the growth of the tumor.Twenty New Zealand White rabbits with tumor size over 3 mm in diameter were randomly divided into 2 groups according to the tumor growth time with those less than 3 weeks as group 1 and those more than 3 weeks as group 2,and perfusion CT were performed accordingly.CT measurements of BV,BF and PS from tumor,peritumor and contralateral normal tissue regions were obtained.After that the animals were sacrificed and 2% Evans blue(2 mL/kg)was given intravenously in 16 of these animals 1 h prior to sacrifice to detect breakdown of the blood brain barrier.VEGF and MVD were evaluated in im-munohistochemical examination of the specimens.Results:Tumor had significantly higher BV,BF and PS(P=0.000)than peritumor and normal tissue region.Tumor BV,BF and MVD in group 2 were significantly higher than that in group 1(P<0.01).Significant linear correlation was found between MVD and BV(r=0.915,P=0.000),MVD and BF(r=0.901,P=0.000),and MVD and PS(r=0.459,P=0.042).We also found a rank correlation between PS and blue stain of tumor(rs=0.861,P=0.000).Conclu-sion:Perfusion CT can distinguish tumor from peritumor and normal tissue clearly,reflect tumor angiogenesis accurately,and provide useful information for the evaluation of brain tumor.
