On-chip 3D rotation of oocyte based on a vibration-induced local whirling flow

We propose a novel on-chip 3D cell rotation method based on a vibration-induced flow.When circular vibration is applied to a microchip with micropillar patterns,a highly localized whirling flow is induced around the micropillars.The direction and velocity of this flow can be controlled by changing the direction and amplitude of the applied vibration.Furthermore,this flow can be induced on an open chip structure.In this study,we adopted a microchip with three micropillars arranged in a triangular configuration and an xyz piezoelectric actuator to apply the circular vibration.At the centre of the micropillars,the interference of the vibration-induced flows originating from the individual micropillars induces rotational flow.Consequently,a biological cell placed at this centre rotates under the influence of the flow.Under three-plane circular vibrations in the xy,xz or yz plane,the cell can rotate in both the focal and vertical planes of the microscope.Applying this 3D cell rotation method,we measured the rotational speeds of mouse oocytes in the focal and vertical planes as 63.7±4.0°s^(−1) and 3.5±2.1°s^(−1),respectively.Furthermore,we demonstrated the transportation and rotation of the mouse oocytes and re-positioned their nuclei into a position observable by microscope.

Design of Electrohydrodynamic Devices with Consideration of Electrostatic Energy

The importance of actuators that can be integrated with flexible robot structures and mechanisms has increased in recent years with the advance of soft robotics.In particular,electrohydrodynamic(EHD)actuators,which have expandable integrability to adapt to the flexible motion of soft robots,have received much attention in the field of soft robotics.Studies have deepened the understanding of steady states of EHD phenomena but nonsteady states are not well understood.We herein observe the development process of fluid in a microchannel adopting a Schlieren technique with the aid of a high-speed camera.In addition,we analyze the behavior of fluid flow in a microchannel that is designed to have pairs of parallel plate electrodes adopting a computational fluid dynamics technique.Results indicate the importance of considering flow generated by electrostatic energy,which tends to be ignored in constructing and evaluating EHD devices,and by the body force generated by the ion-drag force.By considering these effects,we estimate the development process of EHD flow and confirm the importance of considering the generation of vortices and their interactions inside the microchannel during the development of EHD devices.