Umbilical cord blood (UCB) is a current major source of hematopoietic stem cells (HSCs) for cell transplantation therapy. Cell transplantation with HSCs derived from UCB is advantageous over transplantation with HSCs ...Umbilical cord blood (UCB) is a current major source of hematopoietic stem cells (HSCs) for cell transplantation therapy. Cell transplantation with HSCs derived from UCB is advantageous over transplantation with HSCs from adult tissues. However, the low number of HSC derived from a single unit of UCB limits its application. Thus,<i> ex vivo</i> expansion is a good option to create more UCB HSCs for clinical application. The strategies for HSC expansion <i>in vitro</i> focus on mimicking the composition and structure of HSC natural niche by enhancing self-renewal and inhibiting lineage differentiation of HSCs. In the past decade, the mechanisms of the interaction between HSC and the natural niche have been deeply investigated. This great progress in basic research has led to advancements in UCB HSC<i> ex vivo</i> expansion. In addition, the biological characteristics of the originally isolated UCB HSCs correlate with outcome of subsequent<i> ex vivo</i> expansion. In this paper, we summarize the late progress achieved in isolation and<i> ex vivo</i> expansion of UCB HSCs. Importantly, we attempt to provide an impact and practicable procedure to expand UCB HSC <i>in vitro</i> from isolation of original HSCs to identification of expanded HSCs.展开更多
A new method that stabilizes network-based systems with both bounded delay and packet disordering is discussed under the state feedback controller. A novel model, fully describing the dynamic characteristic of network...A new method that stabilizes network-based systems with both bounded delay and packet disordering is discussed under the state feedback controller. A novel model, fully describing the dynamic characteristic of network-based systems with packet disordering, is constructed. Different from the existing models of network-based systems, the number of delay items is time-varying in the model proposed. Further, this model is converted into a parameter-uncertain discrete-time system with time-varying delay item numbers in terms of matrix theory. Moreover, the less conservative stability condition is obtained by avoiding utilisation of Moon et al.’ inequality and bounding inequalities for quadratic functional terms. By solving a minization problem based on linear matrix inequalities, the state feedback controller is presented. A numerical example is given to illustrate the effectiveness of the proposed method.展开更多
文摘Umbilical cord blood (UCB) is a current major source of hematopoietic stem cells (HSCs) for cell transplantation therapy. Cell transplantation with HSCs derived from UCB is advantageous over transplantation with HSCs from adult tissues. However, the low number of HSC derived from a single unit of UCB limits its application. Thus,<i> ex vivo</i> expansion is a good option to create more UCB HSCs for clinical application. The strategies for HSC expansion <i>in vitro</i> focus on mimicking the composition and structure of HSC natural niche by enhancing self-renewal and inhibiting lineage differentiation of HSCs. In the past decade, the mechanisms of the interaction between HSC and the natural niche have been deeply investigated. This great progress in basic research has led to advancements in UCB HSC<i> ex vivo</i> expansion. In addition, the biological characteristics of the originally isolated UCB HSCs correlate with outcome of subsequent<i> ex vivo</i> expansion. In this paper, we summarize the late progress achieved in isolation and<i> ex vivo</i> expansion of UCB HSCs. Importantly, we attempt to provide an impact and practicable procedure to expand UCB HSC <i>in vitro</i> from isolation of original HSCs to identification of expanded HSCs.
基金supported by the National Natural Science Foundation of China (60874057 60725312+3 种基金 61074029)the Liaoning Provincal Foundation of Science and Technology (20082023)the Natural Science Foundation of Liaoning Province (20092083)China Postdoctoral Science Foundation Project (20100471488)
文摘A new method that stabilizes network-based systems with both bounded delay and packet disordering is discussed under the state feedback controller. A novel model, fully describing the dynamic characteristic of network-based systems with packet disordering, is constructed. Different from the existing models of network-based systems, the number of delay items is time-varying in the model proposed. Further, this model is converted into a parameter-uncertain discrete-time system with time-varying delay item numbers in terms of matrix theory. Moreover, the less conservative stability condition is obtained by avoiding utilisation of Moon et al.’ inequality and bounding inequalities for quadratic functional terms. By solving a minization problem based on linear matrix inequalities, the state feedback controller is presented. A numerical example is given to illustrate the effectiveness of the proposed method.
