The interaction between fosfomycin （FOS） and bovine serum albumin （BSA） has been investigated ef- fectively by multi-spectroscopic techniques under physiological pH 7.4. FOS quenched the intrinsic fluorescence of ...The interaction between fosfomycin （FOS） and bovine serum albumin （BSA） has been investigated ef- fectively by multi-spectroscopic techniques under physiological pH 7.4. FOS quenched the intrinsic fluorescence of BSA via static quenching. The number of binding sites n and observed binding constant KA were measured by the fluorescence quenching method. The thermodynamic parameters AG0, AH0 and △S0 were calculated at different temperatures according to the van＇t Hoff equation. The site of binding of FOS in the protein was proposed to be Sudlow＇s site I based on displacement experiments using site markers viz. warfarin, ibuprofen and digitoxin. The distance r between the donor （BSA） and acceptor （FOS） molecules was obtained according to the F^rster theory. The effect of FOS on the conformation of BSA was analyzed using synchronous fluorescence spectra （SFS）, circular dichroism （CD） and 3D fluor- escence spectra. A molecular modeling study further confirmed the binding mode obtained by the ex- perimental studies.展开更多
基金DST New Delhi for providing Innovation in Science Pursuit for Inspired Research (INSPIRE) fellowship (IF110548)
文摘The interaction between fosfomycin （FOS） and bovine serum albumin （BSA） has been investigated ef- fectively by multi-spectroscopic techniques under physiological pH 7.4. FOS quenched the intrinsic fluorescence of BSA via static quenching. The number of binding sites n and observed binding constant KA were measured by the fluorescence quenching method. The thermodynamic parameters AG0, AH0 and △S0 were calculated at different temperatures according to the van＇t Hoff equation. The site of binding of FOS in the protein was proposed to be Sudlow＇s site I based on displacement experiments using site markers viz. warfarin, ibuprofen and digitoxin. The distance r between the donor （BSA） and acceptor （FOS） molecules was obtained according to the F^rster theory. The effect of FOS on the conformation of BSA was analyzed using synchronous fluorescence spectra （SFS）, circular dichroism （CD） and 3D fluor- escence spectra. A molecular modeling study further confirmed the binding mode obtained by the ex- perimental studies.
